PHYSICOCHEMICAL CHARACTERISTICS OF RASAMANIKYA-AN AYURVEDIC ARSENICAL FORMULATION
Keywords:Physico-chemical, Rasamanikya, Ayurvedic arsenical
Objective: The objective of this study was standardization and Chemical characterization of rasamanikya prepared as per standard operating procedures (SOP) mentioned in the classical text.
Methods: Rasamanikya was prepared by putting churnodaka shodhita haratala (Orpiment-As2S3) between two abhraka (white mica) sheets which are heated for a while to obtain a red colored finished product. The Ayurvedic specifications for the analysis of rasamanikya were performed through qualitative and quantitative analysis. Physicochemical analysis, assay of elements by atomic absorption spectrometer (AAS) and inductively coupled plasma-atomic emission spectrometry (ICP-AES) were carried out and some other tests such as x-ray diffraction (XRD), x-ray photoelectron spectroscopy (XPS) and energy dispersive x-ray analyzer (EDAX) were also performed to ensure the quality of the drug.
Results: In the finished drug Arsenic and Sulphur are present in the form of As4S4, As2S3, As2S5. On the basis of XPS survey scans, scanning electron microscopy-energy dispersive x-ray analyzer (SEM-EDAX) and carbon, hydrogen, nitrogen, sulphur (CHNS) analysis the Arsenic to Sulphur (As to S) ratio is thus standardized as 39-47: 53-61. In addition to this powder, XRD shows a major conversion into an amorphous phase.
Conclusion: The results could be used to lay down a new set of pharmacopoeial standards for the preparation of rasamanikya for getting optimal efficacy of medicine. Therefore, the information will help the Scientists and Researchers to build comprehensive standards, to screen the compounds responsible for different bioactivities, and to elucidate the molecular mechanism of action.
Dhundhukanath A. Rasendra chintamani. In: Mishra SN. editors. Vol. 9. Hindi commentary. Varanasi: Chaukhamba Orientallia; 2000. p. 376.
Bhatt K. Siddhabheshaja manimala. In: Bhatta RK. editors. Vaishwanara Hindi commentary. 3rd ed. Vol. 4. Varanasi: Chaukhamba Krishnadas Academy; 1999. p. 159–60.
Bhatta KG. Rasendra sara sangraha. In: Tripathi ID. editor. Hindi commentary. 2nd ed. Vol. 1. Varanasi: Chaukhamba Orientallia; 1998. p. 48–9.
Sharma S. Rasa Tarangani. In: Pt. Kashinath Shashtri. editor. Hindi commentary. Taranga 11, verse 88-89. 11th ed. Varanasi: Motilal Banarasidas publications; 1979. p. 257-8.
Agnivesha. Charak Samhita. In: Acharya YT. editor. Ayurvedic deepika commentary of chakrapani datta. 5th ed. Varanasi: Chaukhamba Samhita Sansthana; 1981. p. 20.
Shushruta. Shushruta Samhita. In: Acharya YT. editor. Nibhandha Sangraha, Commentray of Dalhana. 8th ed. Varanasi: Chaukhamba Orientalia; 2005. p. 564.
Anonymous. The Ayurvedic Formulary of India (AFI). Part I. 2nd ed. New Delhi: The Controller of Publications (Ministry of Health and Family Welfare, Govt. of India); 2003. p. 270.
Sharma S. Rasa Tarangani. In: Pt. Kashinath Shashtri. editor. Hindi commentary. Taranga 11, verse 88-89. 11th ed. Varanasi: Motilal Banarasidas publications; 1979. p. 280.
Vagbhatta. Rasa Ratna Samuchchaya. In: Shashtri Ambikadutt. editor. Hindi commentary. Adhyaya 09, verse 03-04. 9th ed. Varanasi: Amarbharti Prakashan; 1995. p. 147.
Vagbhatta. Rasa Ratna Samuchchaya. In: Shashtri Ambikadutt. editor. Hindi commentary. Adhyaya 03, verse 74. 9th ed. Varanasi: Amarbharti Prakashan; 1995. p. 73.
Bhatt K. Siddhabheshaja Manimala. In: Bhatta RK. editors. Vaishwanara Hindi commentary. 3rd ed. Vol. 4. Varanasi: Chaukhamba Krishnadas Academy; 1999. p. 159–60.
Anonymous. Pharmacopoeial standards for Ayurvedic formulations. Revised Edition. Delhi: The Controller of Publications (CCRAS, Ministry of Health and Family Welfare, Govt. of India); 1987.
Lohar DR. Protocol for testing of Ayurveda Sidhha and Unani medicine. New Delhi: Pharmacopoeial Laboratory for Indian Medicine, Deptt of AYUSH; 2007.
Lohar DR. Quality Control Manual for Ayurveda Sidhha and Unani Medicine. New Delhi: Pharmacopoeial Laboratory for Indian Medicine, Department of AYUSH; 2008.
Lavekar GS. Laboratory guide for analysis of ayurveda and siddha formulation. New Delhi: Central Council for Research in Ayurvedic Sciences, Department of AYUSH; 2010.
Kumar A, Nair GC, Reddy AVR, Garg AN. Availability of essential elements in bhasmas: analysis of ayurvedic metallic preparations by INAA. J Radio Anal Nucl Chem 2006;270:173–80.
Krishnamurthy V, Sane RT. Study on ayurvedic bahamas on the basis of modern analytical instrumentation techniques. Indian Res J Chem Environ 2001;5:65–7.
Gaidhani SN. Validation and quality assessment of rasamanikya-a classical herbo-mineral preparation. J Sci Ind Res 2011;70:871-4.
K Srimannarayana, Guide PK Prajapati. PhD thesis on pharmaceutical standardization and toxicity study of Rasa Manikya. Jamnagar: IPGT and RA (Department of Rasasashtra); 2012.