ISOLATION AND SCREENING OF ALKALI TOLERANT TRICHODERMA SPP AS BIOCONTROL AGENT FOR ALKALINE AGRICULTURAL SOIL
Objective: The main aim of the study was to screen twenty isolates of Trichoderma spp isolated from high alkaline agricultural soil and non agricultural soil and to test them invitro for their pH levels tolerance and biomass production.
Methods: All the twenty Trichoderma spp isolates were assessed for growth and biomass yield at different alkaline pH 9, 10, 11 on nutrient medium PCA and PCB. The growth were measured in terms of mycelial weight (cell biomass) and sporulation. The most tolerant isolates were further studied for their biocontrol activity against phytopathogen isolated from same location.
Results: Significant variations in the growth of the isolates were recorded with increasing pH. Different isolates of same species also varies in their growth and biomass yield at pH 7, 9, 10, and 11. The isolates were screened for highest growth at pH 11 in terms of biomass yield. 12 isolates showed prominent biomass yield at pH 10.0, but only 5 isolates were able to grow prominently at pH 11
Conclusion: The study concluded that Trichoderma spp isolated from alkali soil with a high pH of more than 11 was only able to grow under high pH range of 9, 10, 11 and also exhibited maximum antagnostic activity for the pathogens studied.
Conclusion:Â The study concluded thatÂ Trichoderma sppÂ isolated from alkali soil with a high pH of more than 11 was only able to grow under high pH range of 9, 10, 11 and also exhibited maximum antagnostic activity for the pathogens studied.
2. Harman GE, Howell CR, Viterbo A, Chet I, Lorito M. "Trichoderma speciesâ€”opportunistic avirulent plant symbionts". Nat Rev Microbiol 2004;2(1):43â€“56.
3. John. Bissett-A revision of the genus Trichoderma II. Infrageneric classification. Canadian J Bot 1991;69(11):2357-72.
4. J DluÅ¼niewska. Reaction of fungi of Trichoderma genus to selected abiotic factors. Electronic J Agricultural University 2003;6:04.
5. Sarojini C Koll. Growth response of Trichoderma isolates against varying pH levels. Int J Environ Biol; 2012.
6. L. Kredics. Influence of environmental parameters on Trichoderma spp strains with biocontrol potential Trichoderma spp Strains with Biocontrol Potential. Food Technol Biotechnol 2003;41(1):37â€“42.
7. S Bandyopadhyay, S Jash, S Dutta. Effect of different pH and temperature levels on growth and sporulation of Trichoderma spp. Environ Ecol 2003;21:770-3.
8. J Bisset. A revision of the genus Trichoderma II. Infragernic classification, Canadian. J Bot 1991a ;69:2357-72.
9. J Bisset. A revision of the genus Trichoderma III. Section Pachybasium, Canadian. J Bot 1991b ;69:2373-417.
10. J Bisset. A revision of the genus Trichoderma III. Additional notes on section Longibrachiatum, Canadian. J Bot 1991c;69:2418-20.
11. Nagamani IK Kunwar C. Manoharachary, Handbook of soil fungi, IK. International Private Limited: New Delhi; 2006. p. 409-25.
12. GJ Samuels, O Petrini, K Kuhls, E Lieckfeldt, CP Kubicek. The Hypocrea schweinitzii complex and Trichoderma sect. Longibrachiatum, Studies Mycol 1998;41:1-54.
13. Sarojini C Koll. Growth response of Trichoderma isolates against varying pH levels. Int J Environ Biol; 2012.
14. T Yamanaka. The effect of pH on the growth of saprophytic and ectomycorrhizal ammonia fungi in vitro. Mycol 2003;95:584-9.