PHYTOCHEMICAL PROFILING USING LC-Q-TOF-MS ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF A RARE SALT-SECRETING MANGROVE AEGIALITIS ROTUNDIFOLIA ROXB. LEAVES EXTRACT
Objective: The present work deals with the qualitative study of the phytoconstituents present in Aegialitis rotundifolia Roxb., ethanolic leaves extract and evaluate its antioxidant properties in vitro.
Methods: The qualitative phytochemical analysis of the extract was performed first using preliminary phytochemical tests and then by liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS). The antioxidant properties were investigated comprehensively using seven in vitro models viz., 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide (H2O2) scavenging, superoxide (SOD) radical scavenging, lipid peroxidation (LPO) assay, reducing power (RP), and total antioxidant activity.
Results: The preliminary phytochemical analysis revealed the presence of several important phytochemical groups whereas the LC-Q-TOF-MS analysis detected 25 phytoconstituents in the extract mostly belonging to flavonoids and alkaloids. The test extract showed strong dose-dependent antioxidant activity in all the seven in vitro models, however, the activity of the extracts was slightly lower compared to the reference standard ascorbic acid.
Conclusion: The test extract showed strong antioxidant properties which could be possibly due to the phytoconstituents detected in the extract.
2. Pham Huy LA, He H, Pham Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008;4:89-96.
3. Geetha S, Irulandi K, Mehalingam P. Evaluation of antioxidant and free radical scavenging activities of different solvent extracts of leaves of Piper umbellatum. Asian J Pharm Clin Res 2017;10:274-6.
4. Taghvaei M, Jafari SM. Application and stability of natural antioxidants in edible oils in order to substitute synthetic additives. J Food Sci Technol 2015;52:1272-82.
5. George M, Britto SJ. Phytochemical, antioxidant and antibacterial studies on the leaf extracts of Curcuma amada Roxb. Int J Curr Pharm Res 2016;8:32-8.
6. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacog Rev 2010;4:118-26.
7. Zhao HX, Zhang HS, Yang SF. Phenolic compounds and its antioxidant activities in ethanolic extracts from seven cultivars of Chinese jujube. Food Sci Human Wellness 2014;3:183-90.
8. Bandaranayake WM. Bioactivities, bioactive compounds and chemical constituents of mangrove plants. Wetl Ecol Manag 2002;10:421-52.
9. Ghosh D, Mondal S, Ramakrishna K. Pharmacobotanical, physicochemical and phytochemical characterisation of a rare salt-secreting mangrove Aegialitis rotundifolia Roxb., (Plumbaginaceae) leaves: a comprehensive pharmacognostical study. S Afr J Bot 2017;113:212–29.
10. Bandaranayake WM. Traditional and medicinal uses of mangroves. Mangroves Salt Marshes 1998;2:133-48.
11. Ray T. Customary use of mangrove tree as a folk medicine among the sundarban resource collectors. Int J Res Humanities Arts Literature 2014;2:43-8.
12. Raju GS, Moghal MMR, Hossain MS, Hassan MM, Billah MM, Ahamed SK, et al. Assessment of pharmacological activities of two medicinal plant of Bangladesh: Launaea sarmentosa and Aegialitis rotundifolia roxb in the management of pain, pyrexia and inflammation. Biol Res 2014;47:55.
13. Reddy ARK, Grace JR. In vitro evaluation of antioxidant activity of Brugeiera gymnorrhiza and Aegialitis rotundifolia. Med Aromat Plants 2016;5:231.
14. Sett S, Mahish C, Poirah I, Dutta D, Mitra A, Mitra AK. Antifungal activity of Aegialitis rotundifolia extract against pathogenic fungi Mycovellosiella. World J Pharm Res 2014;3:403-17.
15. Sett S, Hazra J, Datta S, Mitra A, Mitra AK. Screening the Indian sundarban mangrove for antimicrobial activity. Int J Sci Innovations Discoveries 2014;4:17-25.
16. Hasan I, Hussain MS, Millat MS, Sen N, Rahman MA, Rahman MA, et al. Ascertainment of pharmacological activities of Allamanda neriifolia hook and Aegialitis rotundifolia roxb used in Bangladesh: an in vitro study. J Tradit Complement Med 2018;8:107-12.
17. Reddy ARK, Grace JR. Anticancer activity of methanolic extracts of selected mangrove plants. Int J Pharm Sci Res 2016;7:3852-6.
18. Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. Chapman and Hall. New York; 1984.
19. Kokate CK. Practical pharmacognosy. Fourth ed. Vallabh Prakashan, Delhi, India; 1994.
20. Mondal S, Ghosh D, Ganapaty S, Chekuboyina SV, Samal M. Hepatoprotective activity of Macrothelypteris torresiana (Gaudich.) aerial parts against CCl4-induced hepatotoxicity in rodents and analysis of polyphenolic compounds by HPTLC. J Pharm Anal 2017;7:181–9.
21. Zengin G, Cakmak YS, Guler GO, Aktumsek A. In vitro antioxidant capacities and fatty acid compositions of three centaurea species collected from central anatolia region of turkey. Food Chem Toxicol 2010;48:2638–41.
22. Marcocci L, Maguire JJ, Droy Lefaix MT, Packer L. The nitric oxide-scavenging properties of Ginko bilboa extract EGb 761. Biochem Biophys Res Commun 1994;201:748-55.
23. Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003–8.
24. Dasgupta N, De B. Antioxidant activity of Piper betel L. leaf extract in vitro. Food Chem 2004;88:219-24.
25. Zhou G, Chen Y, Liu S, Yao X, Wang Y. In vitro and in vivo hepatoprotective and antioxidant activity of ethanolic extract from Meconopsis integrifolia (Maxim.) Franch. J Ethnopharmacol 2013;148:664–70.
26. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of Vitamin E. Anal Biochem 1999;269:337–41.
27. Archana D, Dixitha M, Santhy KS. An antioxidant and anti clastogenic potential of Piper longum L. Int J Appl Pharm 2015;7:11-4.
28. Mahajan S, Tuteja N. Cold, salinity and drought stresses an overview. Arch Biochem Biophys 2005;444:139–58.
29. Thatoi HN, Patra JK, Das SK. Free radical scavenging and antioxidant potential of mangrove plants: a review. Acta Physiol Plant 2014;36:561-79.
30. Zhou JL, Qi LW, Li P. Herbal medicine analysis by liquid chromatography/time-of-flight mass spectrometry. J Chromatogr A 2009;1216:7582–94.
31. Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the antioxidant potential of medicinal plant species. Food Bioprod Process 2011;89:217-33.
32. Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J 2013;21:143-52.
33. Gulcin I, Berashvili D, Gepdiremen A. Antiradical and antioxidant activity of total anthocyanins from Perilla pankinensis decne. J Ethnopharmacol 2005;101:287-93.
34. Kasangana PB, Haddad PS, Stevanovic T. Study of polyphenol content and antioxidant capacity of Myrianthus Arboreus (Cecropiaceae) root bark extracts. Antioxidants 2015;4:410-26.
35. Alves CQ, David JM, David JP, Bahia MV, Aguiar RM. Methods for determination of in vitro antioxidant activity for extracts and organic compounds. Quim Nova 2010;33:2202-10.
36. Meyer AS, Isaksen A. Application of enzymes as food antioxidants. Trends Food Sci Technol 1995;6:300-4.
37. Gutteridge JM. Age pigment and free radicals. Fluorescent lipid complexes formed by Iron and copper containing proteins. Biochim Biophys Acta 1985;834:144-8.
38. Gordon MH. The mechanism of antioxidant action in vitro. In: B. J. Hudson (Eds.), Food antioxidants. Elsevier Applied Science, London; 1990. p. 1-18.
39. Zengin G, Nithiyanantham S, Locatellic M, Ceylan R, Uysal S, Aktumsek A, et al. Screening of in vitro antioxidant and enzyme inhibitory activities of different extracts from two uninvestigated wild plants: Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. Auriculata. Eur J Integr Med 2016;8:286-92.
40. Srinivas K, Jimoh FO, Grierson DS, Afolayan AJ. Antioxidant activity of two steroid alkaloids extracted from Solanum aculeastrum. J Pharmacol Toxicol 2007;2:160-7.
41. Wilmsen PK, Spada DS, Salvador M. Antioxidant activity of the flavonoid hesperidin in chemical and biological systems. J Agric Food Chem 2005;53:4757-61.
42. Record IR, Dreosti IE, McInerney JK. The antioxidant activity of genistein in vitro. J Nutr Biochem 1995;6:481-5.
43. Yu J, Wang L, Walzem RL, Miller EG, Pike LM, Patil BS. Antioxidant activity of citrus limonoids, flavonoids, and coumarins. J Agric Food Chem 2005;53:2009-14.