SUPPLEMENTATION OF CALCIUM AND SELENIUM AGAINST CADMIUM INDUCED BIOACCUMULATION IN SELECTED TISSUES OF FRESH WATER FISH, OREOCHROMIS MOSSAMBICUS
Objective: Cadmium (Cd) is one of the most hazardous heavy metals in aquatic environments and could threaten aquatic organisms including fish. The present study was carried out to know the protective effects of calcium (Ca) and selenium (Se) in reducing the Cd bioaccumulation in selected tissues of fresh water fish, Oreochromis mossambicus.
Methods: The fresh water fish, Oreochromis mossambicus (Tilapia) was brought from the local ponds and were allowed for acclimatization to the laboratory conditions. After acclimatization, fish were exposed to sublethal concentration of Cd (1/10th of LC50/48h, i.e., 5 ppm) for 7, 15 and 30 d (d) period. 15d Cd-exposed fish were divided into three groups. The first group of fish were subjected to Ca (1 ppm) supplementation, second group received only Se (1 ppm) supplement and third group of fish were supplemented with the combination of both Ca and Se at the above said doses and observed for 7, 15 and 30d time periods. After specific time intervals, liver, kidney, gill and intestine tissues were isolated and used for Cd bioaccumulation studies.
Results: Cd concentration levels significantly (P<0.05) increased in the test tissues with increased period of exposure. Maximum Cd accumulation was found in 30d Cd-exposed fish kidney tissue (22.611Â±0.676 Î¼g/gm wet wt. of the tissue). However, after supplementing with Ca and/or Se, there was a significant reversal in the levels of Cd concentration in all the test tissues. Maximum reduction was observed under Ca alone supplementation.
Conclusion: The present study clearly reveals that individual supplementation of Ca tends to detoxify the Cd body burden in the test tissues than the other modes of supplementation.
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