SUPPLEMENTATION OF CALCIUM AND SELENIUM AGAINST CADMIUM INDUCED BIOACCUMULATION IN SELECTED TISSUES OF FRESH WATER FISH, OREOCHROMIS MOSSAMBICUS
Objective: Cadmium (Cd) is one of the most hazardous heavy metals in aquatic environments and could threaten aquatic organisms including fish. The present study was carried out to know the protective effects of calcium (Ca) and selenium (Se) in reducing the Cd bioaccumulation in selected tissues of fresh water fish, Oreochromis mossambicus.
Methods: The fresh water fish, Oreochromis mossambicus (Tilapia) was brought from the local ponds and were allowed for acclimatization to the laboratory conditions. After acclimatization, fish were exposed to sublethal concentration of Cd (1/10th of LC50/48h, i.e., 5 ppm) for 7, 15 and 30 d (d) period. 15d Cd-exposed fish were divided into three groups. The first group of fish were subjected to Ca (1 ppm) supplementation, second group received only Se (1 ppm) supplement and third group of fish were supplemented with the combination of both Ca and Se at the above said doses and observed for 7, 15 and 30d time periods. After specific time intervals, liver, kidney, gill and intestine tissues were isolated and used for Cd bioaccumulation studies.
Results: Cd concentration levels significantly (P<0.05) increased in the test tissues with increased period of exposure. Maximum Cd accumulation was found in 30d Cd-exposed fish kidney tissue (22.611±0.676 μg/gm wet wt. of the tissue). However, after supplementing with Ca and/or Se, there was a significant reversal in the levels of Cd concentration in all the test tissues. Maximum reduction was observed under Ca alone supplementation.
Conclusion: The present study clearly reveals that individual supplementation of Ca tends to detoxify the Cd body burden in the test tissues than the other modes of supplementation.
2. Cirillo T, Amodio Cocchieri R, Fasano A, Lucisano A, Tafuri S, Ferrante E, et al. Cadmium accumulation and antioxidant responses in Sparus aurata exposed to waterborne cadmium. Arch Environ Contam Toxicol 2012;62:118-26.
3. Quinaia SP, Rollemberg MCE, Da Silva JBB. On-line preconcentration system using a mini-column of activated carbon for cadmium and lead determination in water by flame atomic absorption spectrometry. Canadian J Animal Sci 2006;51:225-33.
4. Mekkawy IAA, Mahmoud UM, Wassif ET, Naguib M. Effects of cadmium on some haematological and biochemical characteristics of Oreochromis niloticus (Linnaeus, 1758) dietary supplemented with tomato paste and vitamin E. Fish Physiol Biochem 2011;37:71-84.
5. Mekkawy IAA, Lashein FE. The effect of lead and cadmium on LDH and G-6-PDH isozyme patterns exhibited during the early embryonic development of the teleost fish, ctenopharyngodon idellus with emphasis on the corresponding morphological variations. In: The Big Fish Bang: Proceedings of the 26th annual larval fish conference (LFC2002); 2002. HI Browman, Skiftesvik AB. Bergen, Norway; 2003. p. 275-92.
6. Yilmaz AB. Comparison on heavy metal levels of grey mullet (Mugil cephalus L.) and sea beam (Sparus aurata L.) caught in Iskenderum Bay (Turkey). Turkey J Vet Anim Sci 2005;29:257-62.
7. Reynders H, Bervoets L, Gelders M, De Coen WM, Blust R. Accumulation and effects of metals in caged carp and resident roach along a metal pollution gradient. Sci Total Environ 2008;391:82-95.
8. Annabi A, khaled Said K, Messaoudi I. Cadmium: bioaccumulation, histopathology and detoxifying mechanisms in fish. Am J Res Communication 2013;1:60?79.
9. Soeginato A. Bioaccumulation of heavy metals in some commercial animals caught from selected coastal waters of East Java, Indonesia. Res J Agric Biol Sci 2008;4:881-5.
10. Ibrahim S, Said HA. Heavy metals load in tilapia species: a case study of Jakara river and Kusalla dam, Kano state, Nigeria. Bayero J Pure Appl Sci 2010;3:87-90.
11. Vasantharaja D, Ramalingam V. Neurotoxic effect of titanium dioxide nanoparticles: biochemical and pathological approach in male wistar rats. Int J Appl Pharm 2018;10:74-81.
12. Lalitha Devi B, Muthiah NS, Satya Narayana Murty K. Antioxidant activity of conjugated linoleic acid. Asian J Pharm Clin Res 2018;11:169-73.
13. Obaiah Jamakala, Usha Rani A. Protective role of trace elements against cadmium-induced alterations in the selected oxidative stress enzymes in liver and kidney of fresh water teleost, Oreochromis mossambicus (Tilapia). Int J Pharm Pharm Sci 2012;4 Suppl 5:303-10.
14. Obaiah Jamakala, Usha Rani A. Calcium impact on cadmium induced alterations in selected oxidative stress enzymes in the fresh water teleost, Oreochromis mossambicus (Tilapia). Golden Res Thoughts 2013;3:1-6.
15. Obaiah Jamakala, Usha Rani A. Zinc and calcium supplementation to combat cadmium-induced bioaccumulation in selected tissues of fresh water teleost, Oreochromis mossambicus (Tilapia). Int J Universal Pharm Biosci 2014;3:229-42.
16. Ilback NG, Lindh U, Minqin R, Friman G, Watt F. Selenium and mercury are redistributed to the brain during viral infection in mice. Biol Trace Elem Res 2005;108:215-24.
17. Liu CT, Chou MY, Lin CH, Wu SM. Effects of ambient cadmium with calcium on mRNA expressions of calcium uptake related transporters in zebra fish (Danio rerio) larvae. Fish Physiol Biochem 2012;38:977-88.
18. W?odarczyk M, Minta B, Biernacki J, Szkoda ?mudzki J. Selenium protection against cadmium toxicity in hamster embryos. Polish J Environ Studies 2000;9:323-7.
19. Bansal MP, Kaur P. Selenium, a versatile trace element: current research implications. Indian J Exp Biol 2005;43:1119-29.
20. El-Sharaky AS, Newairy AA, Badreldeen MM, Ewedaa SM, Sheweita SA. Protective role of selenium against renal toxicity induced by cadmium in rats. Toxicology 2007;235:185-93.
21. Shilo S, Pardo M, Aharoni Simon M, Glibter S, Tirosh O. Selenium supplementation increases liver MnSOD expression: a molecular mechanism for hepato-protection. J Inorg Biochem 2008;102:110-8.
22. Chen X, Zhu YH, Cheng XY, Zhang ZW, Xu SW. The protection of selenium against cadmium-induced cytotoxicity via the heat shock protein pathway in chicken splenic lymphocytes. Molecules 2012;17:14565-72.
23. Lazarus M, Orct T, Jurasoviæ J, Blanusa M. The effect of dietary selenium supplementation on cadmium absorption and retention in suckling rats. Biometals 2009;22:973-83.
24. Patra RC, Rautray AK, Swarup D. Oxidative stress in lead and cadmium toxicity and its amelioration. Vet Med Int 2011. Doi:10.4061/2011/457327.
25. Zwolak I, Zaporowska H. Selenium interactions and toxicity: a review. Cell Biol Toxicol 2012;28:31-46.
26. Hassan W, Ibrahim M, Nogueira C, Ahmed M, Rocha J. Effects of acidosis and Fe(II) on lipid peroxidation in phospholipid extract: comparative effect of diphenyl diselenide and ebselen. Environ Toxicol Pharmacol 2009;28:152-4.
27. Machado Mda S, Villela IV, Moura DJ, Rosa RM, Salvador M, Lopes NP, et al. 3’3-ditriXuoromethyldiphenyl diselenide: a new organoselenium compound with interesting antigenotoxic and antimutagenic activities. Mut Res 2009;673:133-40.
28. Valdiglesias V, Pasaro E, Mendez JW, LaVon B. In vitro evaluation of selenium genotoxic, cytotoxic, and protective effects: a review. Arch Toxicol 2010;84:337-51.
29. Baldisserotto B, Kamunde C, Matsuo A, Wood C. A protective effect of dietary calcium against acute waterborne cadmium uptake in rainbow trout. Aquat Toxicol 2004;67:57-73.
30. Niyogi S, Kent K, Wood CM. Effects of water chemistry variables on gill binding and acute toxicity of cadmium in rainbow trout (Oncorhynchus mykiss): a biotic ligand model (BLM) approach. Comp Biochem Physiol Part C: Toxicol Pharmacol 2008;148:305-14.
31. Brinkman SF, Hansen DL. Toxicity of cadmium to early life stages of brown trout (Salmo trutta) at multiple water hardnesses. Environ Toxicol Chem 2007;26:1666-71.
32. Hansen JA, Welsh PG, Lipton J, Cacela D, Dailey AD. Relative sensitivity of bull trout (Salvelinus confluentus) and rainbow trout (Oncorhynchus mykiss) to acute exposures of cadmium and zinc. Environ Toxicol Chem 2002;21:67-75.
33. Wu SM, Ho YC, Shih MJ. Effects of Ca2+or Na+on metallothionein expression in tilapia larvae (Oreochromis mossambicus) exposed to cadmium or copper. Arch Environ Contam Toxicol 2007;52:229-34.
34. Ng TYT, Klink JS, Wood CM. Does dietary Ca protect against toxicity of a low dietborne Cd exposure to the rainbow trout? Aquat Toxicol 2009;91:75-86.
35. Grosel M, Wood CM. Copper uptake across rainbow trout gills: mechanisms of apical entry. J Exp Biol 2002;205:1179-88.
36. Kamunde CN, Pyle GG, McDonald DG, Wood CM. Influence of dietary sodium and waterborne copper exposure toxicity and gill copper binding in rainbow trout, Oncorhynchus mykiss. Environ Toxicol Chem 2003;22:342-50.
37. Zohouri MA, Pyle GG, Wood CM. Dietary Ca inhibits waterborne Cd uptake in Cd-exposed rainbow trout, Oncorhynchus mykiss. Comp Biochem Physiol 2001;130C:347-56.
38. Ojo AA, Wood CM. In vitro characterization of cadmium and zinc uptake via the gastro-intestinal tract of the rainbow trout (Oncorhynchus mykiss): effective interactive effects and the influence of calcium. Aquatic Toxicol 2008;89:55-64.
39. Kwong RWM, Niyogi S. The interactions of iron with other divalent metals in the intestinal tract of a fresh water teleost, rainbow trout (Oncorhynchus mykis). Comp Biochem Physiol C 2009;150:442-9.
40. Usha Rani A, Ramamurthi R. Effect of sublethal concentration of cadmium on oxidative metabolism in the fresh water teleost Tilapia mossambica. Indian J Comp Anim Physiol 1987;5:71-4.
41. Kanno S, Aoki Y, Suzuki JS, Takeichi N, Misawa S, Suzuki KT. Enhanced synthesis of metallothionein as a possible cause of abnormal copper accumulation in LEC rats. J Inorg Biochem 1994;56:117-25.
42. Francis PC, Birge WJ, Black JA. Effects of cadmium enriched sediment on fish and amphibian embryo?larval stages. Fish Physiol Biochem 2004;36:403-9.
43. Kamunde C. Early subcellular partitioning of cadmium in the gill and liver of rainbow trout (Oncorhynchus mykiss) following low-to-near-lethal waterborne cadmium exposure. Aquat Toxicol 2009;91:291-301.
44. Zahedi S, Mirvaghefi A, Rafati M, Mehrpoosh M. Cadmium accumulation and biochemical parameters in juvenile persian sturgeon, Acipenser persicus, upon sublethal cadmium exposure. Comp Clin Pathol 2013;22:805–13.
45. Isani G, Andreani G, Cocchioni F, Fedeli D, Carpene E, Falcioni G. Cadmium accumulation and biochemical responses in Sparus aurata following sub?lethal Cd exposure. Ecotoxicol Environ Safe 2009;72:224-30.
46. Creti P, Trinchella F, Scudiero R. Heavy metal bioaccumulation and metallothionein content in tissues of the sea bream Sparus aurata from three different fish farming systems. Environ Monit Assess 2010;165:321-9.
47. Obaiah Jamakala, Bhavani G, Usha Rani A. Effect of calcium on cadmium induced bioaccumulation in selected tissues of fresh water teleost, Oreochromis mossambicus (Tilapia). Indian Streams Res J 2013;3:56-9.
48. Obaiah Jamakala, Usha Rani A. Metallothioneins role against cadmium toxicity in zinc and calcium supplemented Oreochromis mossambicus (Tilapia). Int J Pharma Bio Sci 2015;6:149-63.
49. Obaiah Jamakala, Usha Rani A. Zinc and calcium supplementation to combat cadmium induced bioaccumulation in fresh water teleost Oreochromis mossambicus (Tilapia). Int J Pharm Pharm Sci 2016;8:186-90.
50. Obaiah Jamakala, Usha Rani A. Possible detoxification role of zinc and selenium supplementation against cadmium induced bioaccumulation and oxidative stress in teleostean fish Oreochromis mossambicus. Eur J Pharm Med Res 2017;4:370-82.
51. Pretto A, Loro VL, Baldisserotto B, Pavanato MA, Moraes BS, Menezes C, et al. Effects of water cadmium concentrations on bioaccumulation and various oxidative stress parameters in Rhamdia quelen. Arch Environ Contam Toxicol 2011;60:309-18.
52. Klaassen CD, Liu J, Diwan BA. Metallothionein protection of cadmium toxicity. Toxicol Appl Pharmacol 2009;238:215-20.
53. WHO. Environmental health criteria, 134, cadmium. World Health Organization, Geneva; 1992.
54. Jayakumar P, Paul VI. Patterns of cadmium accumulation in selected tissues of the catfish Clarias batracus (Linn.) exposed to sublethal concentration of cadmium chloride. Vet Arch 2006;76:167-77.
55. Jezierska B, Witeska M. The metal uptake and accumulation in fish living in polluted waters. Soil and Water Pollution Monitoring, Protection and Remediation; 2006. p. 107-14.
56. Giari L, Manera M, Simoni E, Dezfuli BS. Cellular alterations in different organs of European sea bass Dicentrarchus labrax (L.) exposed to cadmium. Chemosphere 2007;67:1171-81.
57. Turgut S, Polat A, Inan M, Turgut G, Emmungil G, Bican M, et al. Interaction between anemia and blood levels of iron, zinc, copper, cadmium, and lead in children. Indian J Pediatrics 2007;74:827-30.
58. Renata SK, Izabela H. Cadmium, zinc and iron interactions in the tissues of bank vole Clethrionomys glareolus after exposure to low and high doses of cadmium chloride. Biometals 2007;20:743-9.
59. Peraza MA, Ayala Fierro F, Barber DS, Casarez E, Rael LT. Effects of micronutrients o n metal toxicity. Environ Health Perspect 1998;106:203-16.
60. Flora SJS, Mittal M, Mehta A. Heavy metal induced oxidative stress and its possible reversal by chelation therapy. Indian J Med Res 2008;128:501-23.
61. Asagba SO. Role of diet in absorption and toxicity of oral cadmium–A review of the literature. Afr J Biotechnol 2009;8:7428-36.
62. Talas ZS, Orun I, Ozdemir I, Erdogan K, Alkan A, Yilmaz I. Antioxidative role of selenium against the toxic effect of heavy metals (Cd+2, Cr+3) on the liver of rainbow trout (Oncorhynchus mykiss walbaum 1792). Fish Physiol Biochem 2008;34:217-22.
63. Gailer J. Chronic toxicity of AsIII in mammals: the role of (GS) 2AsSe?. Biochimie 2009;91:1268-72.
64. Tawwab MA, Wafeek M. Response of nile tilapia, Oreochromis niloticus (L.) to environmental cadmium toxicity during organic selenium supplementation. 8th International Symposium on Tilapia in Aquaculture; 2008. p. 415-30.
65. Cogun HY, Ozgur Firat, Ozge Firat, Yuzereroglu TA, Gok G, Kargin F, et al. Protective effect of selenium against mercury-induced toxicity on hematological and biochemical parameters of Oreochromis niloticus. J Biochem Mol Toxicol 2012;26:117-22.
66. Banni M, Chouchene L, Said K, Kerkeni A, Messaoudi I. Mechanisms underlying the protective effect of zinc and selenium against cadmium induced oxidative stress in zebra fish Danio rerio. Biometals 2011;24:981-92.
67. Flora SJS. Metal poisoning: threat and management. Al Ameen J Med Sci 2009;2:4-26.
68. Krishnammal S, Navaraj PS. A dietary chemical study of the protection of calcium against cadmium uptake in freshwater fish. IOSR J Pharm Biol Sci 2012;3:19-26.
69. Franklin NM, Glover CN, Nicol JA, Wood CM. Calcium/cadmium interactions at uptake surfaces in rainbow trout: waterborne versus dietary routes of exposure. Environ Toxicol Chem 2005;24:2954-64.
70. Hollis L, McGeer JC, McDonald DG, Wood CM. Protective effects of calcium against chronic waterborne cadmium exposure to juvenile rainbow trout. Environ Toxicol Chem 2000;19:2725-34.
71. Ghosh L, Adhikari S. Accumulation of heavy metals in fresh water fish–an assessment of toxic interactions with calcium. Am J Food Technol 2006;1:139-48.