• Shilpee Chanda Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai, Maharashtra 400019, India
  • Archana R. Juvekar Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai, Maharashtra 400019, India


Objective: The present study was carried out to investigate the in vitro anti-inflammatory activity of syringic acid (SA). 

Methods: SA was tested for it's in vitro anti-inflammatory activity at different concentrations in protein denaturation, proteinase inhibition and human red blood cell (HRBC) membrane stabilization assay. The reference drugs used were aspirin and diclofenac sodium.

Results: SA showed concentration-dependent inhibition of protein denaturation and proteinase activity with a half-maximal inhibitory concentration (IC50) value of 49.38±0.56 µg/ml and 53.73±0.27 µg/ml respectively. Heat-induced haemolysis was inhibited by SA with an IC50 value of 57.13±0.24 µg/ml. SA also inhibited the hypotonicity-induced haemolysis (IC50 value of 53.87±0.72 µg/ml).

Conclusion: From the present study, we can conclude that SA possesses appreciable anti-inflammatory effect against denaturation of proteins, proteinase activity, and human red blood membrane stabilization assays. Further studies are required for determining the possible mechanisms behind its anti-inflammatory action.

Keywords: Syringic acid, Antioxidant, Anti-inflammatory, Protein denaturation, Proteinase inhibition, Membrane stabilization


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1. Tortora GJ, Derrickson BH. Principles of anatomy and physiology. John Wiley Sons; 2008.
2. Kumar V, Abbas AK, Fausto N, Robbins SL, Cotran RS. Pathology basis of disease. New York: WB Saunders; 2004.
3. Guo DA, Lu A, Liu L. Modernization of traditional Chinese medicine. J Ethnopharmacol 2012;141:547.
4. Srinivasulu C, Ramgopal M, Ramanjaneyulu G, Anuradha CM, Kumar CS. Syringic acid (SA)?a review of its occurrence, biosynthesis, pharmacological and industrial importance. Biomed Pharmacother 2018;108:547-57.
5. Sevgi K, Tepe B, Sarikurkcu C. Antioxidant and DNA damage protection potentials of selected phenolic acids. Food Chem Toxicol 2015;77:12-21.
6. Jeong JH, Seung TW, Park SK, Park CH, Jin DE, Heo HJ. Learning and memory effect of syringic acid on amyloid-?-induced neurotoxicity in ICR mice. Agric Life Sci 2015;49:233-44.
7. Itoh A, Isoda K, Kondoh M, Kawase M, Kobayashi M, Tamesada M, et al. Hepatoprotective effect of syringic acid and vanillic acid on concanavalin a-induced liver injury. Biol Pharm Bull 2009;32:1215-9.
8. Itoh A, Isoda K, Kondoh M, Kawase M, Watari A, Kobayashi M, et al. Hepatoprotective effect of syringic acid and vanillic acid on CCl4-induced liver injury. Biol Pharm Bull 2010;33:983-7.
9. Karthik G, Angappan M, Vijaya Kumar A, Natarajapillai S. Syringic acid exerts antiangiogenic activity by downregulation of VEGF in zebrafish embryos. Biomed Prev Nutr 2014;4:203-8.
10. Ham JR, Lee HI, Choi RY, Sim MO, Seo KI, Lee MK. Anti-steatotic and anti-inflammatory roles of syringic acid in high-fat-diet-induced obese mice. Food Funct 2016;7:689-97.
11. Karthick G, Vijayakumar AR, Natarajapillai SU. Preliminary study on salubrious effect of syringic acid on apoptosis in human lung carcinoma A549 cells and in silico analysis through docking studies. Asian J Pharm Clin Res 2014;7:46-9.
12. Tanaka T, Kawaguchi N, Zaima N, Moriyama T, Fukuta Y, Shirasaka N. Anti-osteoporotic activity of a syringic acid diet in ovariectomized mice. J Nat Med 2017;71:632-41.
13. Leelaprakash G, Dass SM. In vitro anti-inflammatory activity of methanol extract of enicostemma axillare. Int J Drug Dev Res 2011;3:189-96.
14. Sarkar KB, Kumar R, Reeta, Verma SC, Pal S, Maddi R, et al. Evaluation of in vitro anti-inflammatory activity and HPTLC analysis of plant Phyllanthus Fraternus. Int J Curr Pharm Res 2017;9:198-200.
15. Sen N, Bulbul L, Hussain F, Amin MT. Assessment of thrombolytic, membrane stabilizing potential and total phenolic content of Typha elephantina Roxb. J Med Plants Res 2016;10:669-75.
16. Chalini K, Johnson M, Adaikalaraj G, Vidyarani G, Ramakrishnan P. Anti-inflammatory activity of aqueous extracts of Gracilaria. Int J Curr Pharm Res 2017;9:17-9.
17. Ingle PV, Patel DM. C-reactive protein in various disease condition–an overview. Asian J Pharm Clin Res 2011;4:9-13.
18. Robertson TB. The physical chemistry of the proteins. New York and London; 1918.
19. Grant NH, Alburn HE, Kryzanauskas C. Stabilization of serum albumin by anti-inflammatory drugs. Biochem Pharmacol 1970;19:715-22.
20. Das SN, Chatterjee S. Long term toxicity study of ART-400. Indian J Indigenous Med 1995;16:117-23.
21. Rastogi S, Iqbal MS, Ohri D. In vitro study of anti-inflammatory and antioxidant activity of some medicinal plants and their interrelationship. Asian J Pharma Clin Res 2018;11:195-202.
22. Sermakkani M. Evaluation of phytochemical and antibacterial activity of Pedalium murex Linn. root. Int Res J Pharm 2011;2:131-4.
23. Shelke TT, Bhaskar VH, Adkar PP, Jha U, Oswal RJ. Anti-microbial activities of Pedalium murex Linn on microbial pathogens. Int J Res Ayurveda Pharm 2011;2:1255-7.
24. Chamlagai D, Singh B. Study of in vitro anti-inflammatory activity of ethnomedicinal plants of sikkim Viscum articulatum and Acorus calamus. Asian J Pharm Clin Res 2016;9:119-22.
25. Azeem AK, Dilip C, Prasanth SS, Shahima VJ, Sajeev K, Naseera C. Anti-inflammatory activity of the glandular extracts of Thunnusalalunga. Asian Pac J Trop Med 2010;3:412-20.
26. Chippada SC, Vangalapati M. Antioxidant, an anti-inflammatory and anti-arthritic activity of Centella asiatica extracts. J Chem Biol Phys Sci 2011;1:260.
27. Kumar V, Bhat ZA, Kumar D, Khan NA, Chashoo IA. Evaluation of anti-inflammatory potential of leaf extracts of Skimmia anquetilia. Asian Pac J Trop Biomed 2012;2:627-30.
28. Lee MH, Kang H, Lee K, Yang G, Ham I, Bu Y, et al. The aerial part of Taraxacum coreanum extract has an anti-inflammatory effect on peritoneal macrophages in vitro and increases survival in a mouse model of septic shock. J Ethnopharmacol 2013;146:1-8.
29. Costa MF, Jesus TI, Lopes BR, Angolini CF, Montagnolli A, de Paula Gomes L, et al. Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation, and NET release. BMC Complem Altern M 2016;16:403.
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How to Cite
Chanda, S., and A. R. Juvekar. “IN VITRO ANTI-INFLAMMATORY ACTIVITY OF SYRINGIC ACID”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 2, Feb. 2019, pp. 71-73, doi:10.22159/ijpps.2019v11i2.30387.
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