EFFECT OFCO-ADMINISTRATION OFEMBLICA OFFICINALIS AND AEGLE MARMELOS EXTRACTS FOR ANTIOXIDANT AND ANTIDIABETIC ACTIVITY

  • Hemant Kumar Jain Department of Quality Assurance Techniques, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune 411041, Maharashtra, India
  • Snehal Jaiswal Department of Pharmaceutical Quality Assurance, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune -411041, Maharashtra, India.

Abstract

Objective: The objective of the present study was to develop an HPLC analytical method and to perform in-vivo study of Emblica officinalis and Aegle marmelos extracts for antioxidant and antidiabetic activity.


Methods: The phyto-chemical analyses, total phenolic content (TPC), TLC, DPPH assay were performed for freeze dried Emblica officinalis fruits aqueous extract (EOFAE) and Aegle marmelos leaves ethyl acetate extract (AMLEAE).The active constituents present in both extracts were estimated by using HPLC system having Hibar® C18 column [250 x4.6 mm, 5µm] and UV detector (264nm). A gradient mobile phase (acetonitrile and water with 0.1% trifluoroacetic acid) was used at flow rate 0.8 ml/min. In-vivo antioxidant, antidiabetic activity of both extracts was conducted on male albino wistar rats for 21 days in streptozotocin-induced diabetic rats (42 rats; n=6).The antidiabetic activity was measured by blood glucose level and biochemical parameters i.e. total cholesterol, triglycerides and total protein. Oxidative stress was measured by antioxidant biomarkers i.e. SOD, GSH, lipid peroxidation by thiobarbituric acid reactive substances method on liver of the experimental rat.


Results: Tannins, saponins, carbohydrate, glycosides are found in EOFAE; coumarins and flavonoids are found in AMLEAE and quinones, phenols are present in both extracts. The values of TPC present in standard gallic acid, EOFAE and AMLEAE were found to be 485.7, 315.6, 300.7mgGAE/g, respectively. Rf values obtained by TLC of EOFAE and AMLEAE were found to be 0.41 and 0.50, respectively. The values of % inhibition shown by EOFAE and AMLEAE in DPPH assay were found to be 97.8%±2 and 95.2%±2, respectively. The values of retention time of EOFAE and AMLEAE by HPLC analysis were found to be 4.59 and 5.28 min, respectively. Histopathological examination of liver was revealed that low dose EOAM (containing of EOFAE 250 + AMLEAE 250 mg/kg body weight) administered once a daily for 21 days showed a significant activity (P˂0.001) with biochemical parameters and antioxidant biomarkers.


Conclusion: The present study showed that the EOFAE and AMLEAE treated group III with (EOAM) low dose 500mg/kg body weight has potent antioxidant and antidiabetic activity.

Keywords: Emblica officinalis, Aegle marmelos, Antioxidant, Antidiabetic activity, Streptozotocin, HPLC

Downloads

Download data is not yet available.

Author Biography

Hemant Kumar Jain, Department of Quality Assurance Techniques, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune 411041, Maharashtra, India

Professor and Head

References

1. www.who.int/news-room/fact-sheets/details/diabetes15 November 2017 [Accessed 25 Dec 2017]
2. https://auroushealthcare.wordpress.com/2018/05/11/icmr-issues-new-guidelines-for-type-2-diabetes-management-as-patient-numbers-touch-72-9-million [Accessed 25 Aug 2018]
3. Anjana RM, Deepa M, Pradeepa R, Mahanta J, Narain K, Das HK, et al. Prevalence of diabetes and prediabetes in 15 states of India: results from the ICMR–INDIAB population-based cross-sectional study. The Lancet Diabetes & Endocrinology. 2017; 5:585-96.
4. Yokota N, Miyakoshi T, Sato Y, Nakasone Y, Yamashita K, Imai T, et al. Predictive models for conversion of prediabetes to diabetes. Journal of diabetes and its complications. 2017;31:1266-71.
5. http://www.who.int/mediacentre/factsheets/fs138/en [Accessed 8 April 2018]
6. www.cdc.gov/diabetes/pdfs/data/statistics/national-diabetes-statistics-report [Accessed 6 Aug 2017]
7. https://www.diabetes.co.uk/features/diabetes-medication-side-effects.html [Accessed 6 May 2018]
8. Nain P, Saini V, Sharma S, Nain J. Antidiabetic and antioxidant potential of Emblica officinalis Gaertn. leaves extract in streptozotocin-induced type-2 diabetes mellitus (T2DM) rats. Journal of Ethnopharmacology. 2012;142:65-71.
9. Narendhirakannan RT, Subramanian S. Biochemical evaluation of the protective effect of Aegle marmelos (L.), Corr. leaf extract on tissue antioxidant defence system and histological changes of pancreatic ?-cells in streptozotocin-induced diabetic rats. Drug and chemical toxicology. 2010;33:120-30.
10. Scartezzini P, Antognoni F, Raggi MA, Poli F, Sabbioni C. Vitamin C content and antioxidant activity of the fruit and of the Ayurvedic preparation of Emblica officinalis Gaertn. Journal of ethnopharmacology. 2006;104:113-8.
11. Ansari A, Shahriar MS, Hassan MM, Das SR, Rokeya B, Haque MA, et al. Emblica officinalis improves glycemic status and oxidative stress in STZ induced type 2 diabetic model rats. Asian Pacific journal of tropical medicine. 2014;7:21-5.
12. Badoni Himani, Sharma Promila, Waheed Syed Mohsin, Singh Saumy. Phytochemical analyses & evaluation of antioxidant, antibacterial & toxic properties of Embilica officinalis & Terminalia bellirica fruit extract. Asian J Pharm Clin Res, 2016:96-102
13. Asaduzzaman et al. Phytochemicals, Nutritional constituent, Antibacterial &Hypogycemic activity of A. Marmelos Lin. Leaf Extract in Alloxan Induced diabetic Rat. Journal of Nutrition & Food Sciences, 2016;4:1-7.
14. Sankeshi V, Kumar PA, Naik RR, Sridhar G, Kumar MP, Gopal VH, et al. Inhibition of aldose reductase by Aegle marmelos and its protective role in diabetic cataract. Journal of ethnopharmacology. 2013;149:215-21.
15. Karmase A, Jagtap S, Bhutani KK. Anti adipogenic activity of Aegle marmelos Correa. Phytomedicine. 2013;20:1267-71.
16. Kesari AN, Gupta RK, Singh SK, Diwakar S, Watal G. Hypoglycemic and antihyperglycemic activity of Aegle marmelos seed extract in normal and diabetic rats. Journal of ethnopharmacology. 2006;107:374-9.
17. Kumar GS, Nayaka H, Dharmesh SM, Salimath PV. Free and bound phenolic antioxidants in amla (Emblica officinalis) and turmeric (Curcuma longa). Journal of food composition and analysis. 2006;19:446-52.
18. Ladva BJ, Mahida VM, Kantaria UD, Gokani RH. Marker based standardization of polyherbal formulation (SJT-DI-02) by high performance thin layer chromatography method. Journal of Pharmacy and Bioallied Sciences. 2014;6:213.
19. Parveen R, Shamsi TN, Singh G, Athar T, Fatima S. Phytochemical analysis and In-vitro Biochemical Characterization of aqueous and methanolic extract of Triphala, a conventional herbal remedy. Biotechnology Reports. 2018;17:126-36.
20. Panaskar SN, Joglekar MM, Taklikar SS, Haldavnekar VS, Arvindekar AU. Aegle marmelos Correa leaf extract prevents secondary complications in streptozotocin?induced diabetic rats and demonstration of limonene as a potent antiglycating agent. Journal of Pharmacy and Pharmacology. 2013;65:884-94.
21. Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the antioxidant potential of medicinal plant species. Food and bioproducts processing. 2011;89:217-33.
22. Karmase A, Prasanna K, Rasabattula S, Bhutani KK. Quantification and comparison of extraction methods for alkaloids in Aegle marmelos leaves by HPLC. Natural product communications. 2014;9:981-3.
23. Sawant L, Prabhakar B, Pandita N. Quantitative HPLC analysis of ascorbic acid and gallic acid in Phyllanthus emblica. J Anal Bio anal Techniques. 2010;1:111.
24. Panicker CY, Varghese HT, Philip D. FT-IR, FT-Raman and SERS spectra of Vitamin C. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2006;65:802-4.
25. Pynam H, Dharmesh SM. Antioxidant and anti-inflammatory properties of marmelosin from Bael (Aegle marmelos L.); Inhibition of TNF-? mediated inflammatory/tumor markers. Biomedicine & Pharmacotherapy. 2018;106:98-108.
26. Usharani P, Fatima N, Muralidhar N. Effects of Phyllanthus emblica extract on endothelial dysfunction and biomarkers of oxidative stress in patients with type 2 diabetes mellitus: a randomized, double-blind, controlled study. Diabetes, metabolic syndrome and obesity: targets and therapy. 2013;6:275.
27. Sabu MC, Kuttan R. Antidiabetic activity of Aegle marmelos and its relationship with its antioxidant properties. Indian Journal of physiology and pharmacology. 2004;48:81-8.
28. Allain CC, Poon LS, Chan CS, Richmond WF, Fu PC. Enzymatic determination of total serum cholesterol. Clinical chemistry. 1974;20:470-5.
29. Bucolo G, David H. Quantitative determination of serum triglycerides by the use of enzymes. Clinical chemistry. 1973;19:476-82.
30. Gornall AC, Bardawal CJ, David MJ. Bio Chem. 1949;177:751
31. Tietz NW, Pruden EL, Siggaard-Andersen O. Tietz Fundamental of Clinical Chemistry.
32. Ellman GL. Tissue sulphydryl groups. Arch Biochem Biophys. 1959;82:70-77.
33. Okhawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric reaction. Anal Biochem.1979;95:351-8.
34. Lowry OH, Rosenbourgh NJ, Farr AL, Randall R. Protein determination using Folin-Ciocalteu reagent. J Biol Chem. 1951;193:265-269.
35. Panda S, Kar A. Synergistic interactions of Aegle marmelos leaf, Emblica officinalis fruit and Ocimum sanctum leaf extracts in the regulation of hyperthyroidism and/or hyperglycaemia. Oriental Pharmacy and Experimental Medicine. 2004;4:37-43.
36. Bihani GV, Rojatkar SR, Bodhankar SL. Investigation of in-vivo analgesic and anti-inflammatory activity in rodents and in-vitro antioxidant activity of extracts of whole plant of Cyathocline purpurea. Int J Pharm Pharm Sci. 2014;6:492-8.
37. Sruthi T, Satyavati D, Upendar K, Kumar CP. Antidiabetic activity and anti-oxidant activity of niddwin, a polyherbal formulation in alloxan induced diabetic rats. Int J Pharm Pharm Sci. 2014;6:273-7.
Statistics
30 Views | Downloads
How to Cite
Jain, H. K., and S. Jaiswal. “EFFECT OFCO-ADMINISTRATION OFEMBLICA OFFICINALIS AND AEGLE MARMELOS EXTRACTS FOR ANTIOXIDANT AND ANTIDIABETIC ACTIVITY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 7, May 2019, https://innovareacademics.in/journals/index.php/ijpps/article/view/30948.
Section
Original Article(s)