EFFECT OFCO-ADMINISTRATION OF EMBLICA OFFICINALIS AND AEGLE MARMELOS EXTRACTS FOR ANTIOXIDANT AND ANTIDIABETIC ACTIVITY
Objective: The objective of the present study was to develop an HPLC analytical method and to perform in vivo study of Emblica officinalis and Aegle marmelos extracts for antioxidant and antidiabetic activity.
Methods: The phytochemical analyses, total phenolic content (TPC), TLC, DPPH assay were performed for freeze-dried Emblica officinalis fruits aqueous extract (EOFAE) and Aegle marmelos leaves ethyl acetate extract (AMLEAE). The active constituents present in both extracts were estimated by using HPLC system having Hibar® C18 column [250 x4.6 mm, 5 µm] and UV detector (264 nm). A gradient mobile phase (acetonitrile and water with 0.1% trifluoroacetic acid) was used at a flow rate of 0.8 ml/min. In vivo antioxidant, antidiabetic activity of both extracts was conducted on male albino Wistar rats for 21 d in streptozotocin-induced diabetic rats (42 rats; n=6). The antidiabetic activity was measured by blood glucose level and biochemical parameters i.e. total cholesterol, triglycerides and total protein. Oxidative stress was measured by antioxidant biomarkers i.e. SOD, GSH, lipid peroxidation by thiobarbituric acid reactive substances method on the liver of the experimental rat.
Results: Tannins, saponins, carbohydrate, glycosides are found in EOFAE; coumarins and flavonoids are found in AMLEAE and quinones, phenols are present in both extracts. The values of TPC present in standard gallic acid, EOFAE and AMLEAE were found to be 485.7, 315.6, 300.7 mgGAE/g, respectively. Rf values obtained by TLC of EOFAE and AMLEAE were found to be 0.41 and 0.50, respectively. The values of % inhibition shown by EOFAE and AMLEAE in DPPH assay were found to be 97.8%±2 and 95.2%±2, respectively. The values of retention time of EOFAE and AMLEAE by HPLC analysis were found to be 4.59 and 5.28 min, respectively. Histopathological examination of the liver was revealed that low dose EOAM (containing of EOFAE 250+AMLEAE 250 mg/kg body weight) administered once a daily for 21 d showed significant activity (P˂0.001) with biochemical parameters and antioxidant biomarkers.
Conclusion: The present study showed that the EOFAE and AMLEAE treated group III with (EOAM) low dose of 500 mg/kg body weight has potent antioxidant and antidiabetic activity.
2. https://auroushealthcare.wordpress.com/2018/05/11/icmr-issues-new-guidelines-for-type-2-diabetes-management-as-patient-numbers-touch-72-9-million. [Last accessed on 25 Aug 2018].
3. Anjana RM, Deepa M, Pradeepa R, Mahanta J, Narain K, Das HK, et al. Prevalence of diabetes and prediabetes in 15 states of India: results from the ICMR–INDIAB population-based cross-sectional study. Lancet Diabetes Endocrinol 2017;5:585-96.
4. Yokota N, Miyakoshi T, Sato Y, Nakasone Y, Yamashita K, Imai T, et al. Predictive models for the conversion of prediabetes to diabetes. J Diabetes Its Complications 2017;31:1266-71.
5. http://www.who.int/mediacentre/factsheets/fs138/en. [Last accessed on 08 April 2018].
6. www.cdc.gov/diabetes/pdfs/data/statistics/national-diabetes-statistics-report. [Last accessed on 06 Aug 2017]
7. https://www.diabetes.co.uk/features/diabetes-medication-side-effects.html. [Last accessed on 06 May 2018]
8. Nain P, Saini V, Sharma S, Nain J. Antidiabetic and antioxidant potential of Emblica officinalis gaertn leaves extract in streptozotocin-induced type-2 diabetes mellitus (T2DM) rats. J Ethnopharmacol 2012;142:65-71.
9. Narendhirakannan RT, Subramanian S. Biochemical evaluation of the protective effect of Aegle marmelos (L.), corr. leaf extract on tissue antioxidant defence system and histological changes of pancreatic ?-cells in streptozotocin-induced diabetic rats. Drug Chem Toxicol 2010;33:120-30.
10. Scartezzini P, Antognoni F, Raggi MA, Poli F, Sabbioni C. Vitamin C content and antioxidant activity of the fruit and of the Ayurvedic preparation of Emblica officinalis Gaertn. J Ethnopharmacol 2006;104:113-8.
11. Ansari A, Shahriar MS, Hassan MM, Das SR, Rokeya B, Haque MA, et al. Emblica officinalis improves glycemic status and oxidative stress in STZ induced type 2 diabetic model rats. Asian Pac J Trop Med 2014;7:21-5.
12. Badoni Himani, Sharma Promila, Waheed Syed Mohsin, Singh Saumy. Phytochemical analyses and evaluation of antioxidant, antibacterial and toxic properties of Embilica officinalis and Terminalia bellirica fruit extract. Asian J Pharm Clin Res 2016;6:96-102.
13. Asaduzzaman. Phytochemicals, nutritional constituent, antibacterial and Hypogycemic activity of A. Marmelos lin. leaf extract in alloxan-induced diabetic rat. J Nutr Food Sci 2016;4:1-7.
14. Sankeshi V, Kumar PA, Naik RR, Sridhar G, Kumar MP, Gopal VH, et al. Inhibition of aldose reductase by Aegle marmelos and its protective role in diabetic cataract. J Ethnopharmacol 2013;149:215-21.
15. Karmase A, Jagtap S, Bhutani KK. Antiadipogenic activity of Aegle marmelos correa. Phytomedicine 2013;20:1267-71.
16. Kesari AN, Gupta RK, Singh SK, Diwakar S, Watal G. Hypoglycemic and antihyperglycemic activity of Aegle marmelos seed extract in normal and diabetic rats. J Ethnopharmacol 2006;107:374-9.
17. Kumar GS, Nayaka H, Dharmesh SM, Salimath PV. Free and bound phenolic antioxidants in amla (Emblica officinalis) and turmeric (Curcuma longa). J Food Composition Anal 2006;19:446-52.
18. Ladva BJ, Mahida VM, Kantaria UD, Gokani RH. Marker based standardization of polyherbal formulation (SJT-DI-02) by high performance thin layer chromatography method. J Pharm Bioallied Sci 2014;6:213.
19. Parveen R, Shamsi TN, Singh G, Athar T, Fatima S. Phytochemical analysis and in vitro biochemical characterization of aqueous and methanolic extract of triphala, a conventional herbal remedy. Biotechnol Rep 2018;17:126-36.
20. Panaskar SN, Joglekar MM, Taklikar SS, Haldavnekar VS, Arvindekar AU. Aegle marmelos correa leaf extract prevents secondary complications in streptozotocin?induced diabetic rats and demonstration of limonene as a potent antiglycating agent. J Pharm Pharmacol 2013;65:884-94.
21. Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the antioxidant potential of medicinal plant species. Food Bioprod Process 2011;89:217-33.
22. Karmase A, Prasanna K, Rasabattula S, Bhutani KK. Quantification and comparison of extraction methods for alkaloids in Aegle marmelos leaves by HPLC. Nat Prod Commun 2014;9:981-3.
23. Sawant L, Prabhakar B, Pandita N. Quantitative HPLC analysis of ascorbic acid and gallic acid in Phyllanthus emblica. J Anal Bio Anal Techniques 2010;1:111.
24. Panicker CY, Varghese HT, Philip D. FT-IR, FT-Raman and SERS spectra of Vitamin C. Spectrochim Acta Part A 2006;65:802-4.
25. Pynam H, Dharmesh SM. Antioxidant and anti-inflammatory properties of marmelosin from Bael (Aegle marmelos L.); inhibition of TNF-? mediated inflammatory/tumor markers. Biomed Pharmacother 2018;106:98-108.
26. Usharani P, Fatima N, Muralidhar N. Effects of Phyllanthus emblica extract on endothelial dysfunction and biomarkers of oxidative stress in patients with type 2 diabetes mellitus: a randomized, double-blind, controlled study. Diabetes Metab Syndr Obes: Targets Ther 2013;6:275.
27. Sabu MC, Kuttan R. Antidiabetic activity of Aegle marmelos and its relationship with its antioxidant properties. Indian J Physiol Pharmacol 2004;48:81-8.
28. Allain CC, Poon LS, Chan CS, Richmond WF, Fu PC. Enzymatic determination of total serum cholesterol. Clin Chem 1974;20:470-5.
29. Bucolo G, David H. Quantitative determination of serum triglycerides by the use of enzymes. Clin Chem 1973;19:476-82.
30. Gornall AC, Bardawal CJ, David MJ. Determination of serum proteins by means of the biuret reaction. Bio Chem 1949;177:751-66.
31. Tietz NW, Pruden EL, Siggaard-Andersen O. Electrolytes, blood gases, and acid-base balance. Tietz Fundamental of Clinical Chemistry; 1986.
32. Ellman GL. Tissue sulphydryl groups. Arch Biochem Biophys 1959;82:70-7.
33. Okhawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric reaction. Anal Biochem 1979;95:351-8.
34. Lowry OH, Rosenbourgh NJ, Farr AL, Randall R. Protein determination using folin-ciocalteu reagent. J Biol Chem 1951;193:265-9.
35. Panda S, Kar A. Synergistic interactions of Aegle marmelos leaf, Emblica officinalis fruit and Ocimum sanctum leaf extracts in the regulation of hyperthyroidism and/or hyperglycaemia. Orient Pharm Exp Med 2004;4:37-43.
36. Bihani GV, Rojatkar SR, Bodhankar SL. Investigation of in vivo analgesic and anti-inflammatory activity in rodents and in vitro antioxidant activity of extracts of whole plant of Cyathocline purpurea. Int J Pharm Pharm Sci 2014;6:492-8.
37. Sruthi T, Satyavati D, Upendar K, Kumar CP. Antidiabetic activity and anti-oxidant activity of niddwin, a polyherbal formulation in alloxan induced diabetic rats. Int J Pharm Pharm Sci 2014;6:273-7.
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