DETERMINATION OF VINDOLINE AND RUTIN CONTENT IN FIVE DIFFERENT MORPHOTYPES OF CATHARANTHUS ROSEUS LEAVES USING HPLC
Objective: To determine total phenolic and flavonoids contents and also quantify vindoline and rutin in different morphotypes of Catharanthus roseus using High-performance liquid chromatography (HPLC) method.
Methods: Total flavonoids content (TFC) was determined by Aluminium chloride colorimetric and total phenolic content (TPC) was estimated by Folin-Ciocalteu reagent assay. The chromatographic separation was done by using a C18 column at room temperature and eluted with a mobile phase consisting of a mixture of phosphate buffer (pH=5.8) and acetonitrile at a flow rate of 1.0 ml/minute and detection was carried out at 254 nm.
Results: TPC and TFC content was found highest in Cr00DP and lowest in Cr00WFSRE. Results also showed that the purple morphotypes Cr00DP gives more vindoline (0.3 mg/g) and rutin (18.57 mg/g) concentration compared to the pink morphotype Cr00PFRE contained 18.3 mg/g rutin and 0.2 mg/g vindoline. White morphotypes contained 0.383 mg/g rutin and 0.004 mg/g vindoline which was significantly less as compared to purple and pink morphotypes.
Conclusion: The plant has significant number of alkaloids and flavonoids. The obtained outcomes from different morphotypes are thus significant for the purpose of vindoline and rutin isolation from Catharanthus roseus plant. These isolated bioactive phytoconstituents are a good candidate for further pharmacological and clinical study.
2. Akther T, Khan SM, Hemalatha S. Extraction of flavonoids from different parts of Couroupita guianensis and its efficacy against pathogenic bacteria. Asian J Pharm Clin Res 2017;10:354-8.
3. Ali J, Das B, Saikia T. Antimicrobial activity of lemon peel (Citrus limon) extract. Int J Curr Pharm Res 2017;9:79-82.
4. Doshi GM, Matthews BD, Chaskar PK. Gas chromatography-mass spectroscopy studies on ethanolic extract of dried leaves of Catharanthus roseus. Asian J Pharm Clin Res 2018;11:336-40.
5. Rischer H, Oresic M, Seppanen Laakso T, Katajamaa M, Lammertyn F, Ardiles Diaz W, et al. Gene to metabolite network for terpenoid indole alkaloid biosynthesis in Catharanthus roseus cells. Proc Natl Acad Sci USA 2006;103:5614-9.
6. Rohanizah Abdul Rahim, Nor Hazwani Ahmad, Khaldun Mohammad Al Azzam, Ishak Mat. Determination and quantification of the vinblastine content in purple, red, and white Catharanthus Roseus leaves using RP-HPLC method. Adv Pharm Bull 2018;8:157-61.
7. Ferreres F, Pereira DM, Valentao P, Oliveira JM, Faria J, Gaspar L. Simple and reproducible HPLC-DAD-ESIMS/MS analysis of alkaloids in catharanthus roseus roots. J Pharm Biomed Anal 2010;51:65-9.
8. Jaleel CA, Manivannan P, Sankar B. Induction of drought stress tolerance by ketoconazole in Catharanthus roseus is mediated by enhanced antioxidant potentials and secondary metabolite accumulation. Colloids Surf B: Biointerfaces 2007;60:201-6.
9. Carew DP, Patterson BD. The effect of antibiotics on the growth of Catharanthus roseus tissue cultures. L Loydia 1970;33:275–7.
10. Harbone JB. Phytochemical methods: a guide to modern techniques of plant analysis. Third Edition. Chapman and Hill, London; 1998. p. 279.
11. Singh S, Kaur R, Sharma SK. Pharmacognostical standardization of the roots of Rumex hastatus D. Don. Asian J Pharm Clin Res 2013;6:126-8.
12. Evans WC. Trease and evans pharmacognosy, Harcourt Brace and company. Asia Pvt. Ltd. Singapore; 1997.
13. Parekh J, Chanda SV. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk J Biol 2007;31:53-8.
14. Ugochukwu SK, Uche A, Ifeanyi O. Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala. G Baker. Asian J Plant Sci Res 2013;3:10-3.
15. Kumar GS, Jayaveera KN, Kumar CKA, Sanjay UP, Swamy BMV, Kumar DVK. Antimicrobial effects of Indian medicinal plants against acne-inducing bacteria. Trop J Pharm Res 2007;6:717-23.
16. Kumar S, Malayaman V, Sindhuja S. Phytochemical screening and antibacterial evaluation of the leaf, flower and seed coat extracts of Cassia alata. L. J Chem Pharm Res 2013;5:740-4.
17. Onwukaeme DN, Ikuegbvweha TB, Asonye CC. Evaluation of phytochemical constituents, antibacterial activities and effect of exudates of Pycanthus angolensis weld warb (Myristicaceae) on corneal ulcers in rabbits. Trop J Pharm Res 2007;6:725-30.
18. Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotech 2005;4:685-8.
19. Aiyegroro OA, Okoh AI. Preliminary phytochemical screening and in vitro antioxidant activities of aqueous extract of Helichrysum longifolium DC. BMC Complementary Altern Med 2010;10:21.
20. Govindasamy C, Srinivasan R. In vitro antibacterial activity and phytochemical analysis of Catharanthus roseus (Linn.) G. Don. Asian Pac J Trop Biomed 2012;2:S155-S158.
21. Dhivya SM, Kalaichelvi K. Phytochemical studies and gas chromatography-mass spectrometry analysis of Sarcostemma brevistigma, wight and Arn. Asian J Pharm Clin Res 2017;10:462-6.
22. Rahim RA, Ahmad NH, Azzam KM, Mat I. Determination and quantification of the vinblastine content in purple, red, and white Catharanthus roseus leaves using RP-HPLC method. Adv Pharm Bull 2018;8:157-61.
23. Rao SA, Ahmed MF. Simultaneous determination of phenolic compounds in Catharanthus roseus leaves by HPLC method. Int J Pharm Sci Res 2014;5:976-81.
24. Tikhomiroff C, Jolicoeur M. Screening of Catharanthus roseus secondary metabolites by high-performance liquid chromatography. J Chromatogr A 2002;955:87–93.
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