FECUNDITY STUDIES OF ANDROGRAPHIS PANICULATA ON DROSOPHILA MELANOGASTER

  • Sheik Mohideen Sahabudeen Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology https://orcid.org/0000-0002-9921-8039
  • Hindol Nag Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology https://orcid.org/0000-0001-8274-8108
  • Suneeti Madhavan Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology
  • Abhinav Chatterjee Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology
  • Pallavi Dan Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology https://orcid.org/0000-0002-5814-2912
  • Mahasweta Bhattacharya Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology
  • Zail Ali Nawaz Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology

Abstract

Objective: Andrographis paniculata is widely used in Asia for various medicinal purposes. The plant has a major bioactive chemical constituent Andrographolide, which exhibits various essential pharmacological properties. Recently, to enhance immunity against viral fevers especially dengue viral fever during monsoon season, Tamil Nadu state government has advised rural health centres to provide a tea or kashayam made from a mixture containing this plant leaf along with selected plant roots and leaves. However, there is concern among the general public population that this plant extract may have a negative impact on potency. This study is being done to investigate the toxic effects of Andrographis paniculata on fecundity and physiological properties of Drosophila melanogaster.


Methods: The flies were treated and mated in pure and mixed form of Andrographis paniculata separately.


Results: The results were obtained in the form of egg count, which was compared to control and the conclusion was obtained that Andrographis paniculata might affect the fecundity and at the same time, it was observed that the developmental span from an egg to an adult fly increased upon treatment in successive generations. More importantly, the effect of pure form plant on the fecundity was found to be significant, while that of the mixed form was not. On mating a treated male with an untreated female and an untreated male with a treated female when the treatment is being discontinued, it can be very well seen that there is no effect on the fecundity of the flies.


Conclusion: Since the continuity of the treatment played a major role on the effect of fecundity, highlighting the importance of the period of treatment and the exposure time of the compound on the fly system, therefore this research suggests that the conception of tea or kashayam extracted from mixed plant content could not have the same effect as the similar concentration of pure Andrographis paniculata and it does not have any acute effect on the fecundity when being consumed over a short period of time in Drosophila melanogaster model.

Keywords: Andrographolide, Acute effect, Continuity, Developmental span, Fecundity, Nilevembu

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References

1. Kong JM, Goh NK, Chia LS, Chia TF. Recent advances in traditional plant drugs and orchids. Acta Pharmacologica Sinica 2003;24:7–21.
2. WHO. WHO guidelines on safety monitoring of herbal medicines in pharmacovigilance systems. World Health Organisation, Geneva; 2004.
3. Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers Neurol 2014;4:1–10.
4. Bodeker G, Ong CK, Grundy C, Burford G, Shein K, Medicine WHOP on T, et al. WHO global atlas of traditional, complementary and alternative medicine. Geneva: World Health Organization; 2005.
5. Bandaranayake WM. Quality control, screening, toxicity, and regulation of herbal drugs in modern phytomedicine, turning medicinal plants into drugs. Books; 2006. p. 25-58.
6. Dalsenter PR, Cavalcanti AM, Andrade AJM, Araújo SL, Marques MCA. Reproductive evaluation of aqueous crude extract of Achillea millefolium L. (Asteraceae) in wistar rats. Reprod Toxicol 2004;18:819–23.
7. Schilter B, Andersson C, Anton R, Constable A, Kleiner J, O’Brien J, et al. Guidance for the safety assessment of botanicals and botanical preparations for use in food and food supplements. Food Chem Toxicol 2003;41:1625–49.
8. Farnsworth BNR, Soejarto DD. Global importance of medicinal. Conservation of Medicinal Plants; 1991. p. 25–52.
9. Dey Y, Ota S, De S, Gaidhani S. Effects of the petroleum ether extract of Amorphophallus paeoniifolius on experimentally induced convulsion in mice. Int J Nutr Pharmacol Neurol Dis 2012;2:132.
10. Dey Y, Gaidhani S, Sarkar P, De S. An overview of angiogenesis and renal cell carcinoma. Int J Nutr Pharmacol Neurol Dis 2012;2:3.
11. Asif M. The role of fruits, vegetables, and spices in diabetes. Int J Nutr Pharmacol Neurol Dis 2011;1:27.
12. Ajaya Kumar R, Sridevi K, Vijaya Kumar N, Nanduri S, Rajagopal S. Anticancer and immunostimulatory compounds from Andrographis paniculata. J Ethnopharmacol 2004;92:291–5.
13. Solomon Jeeva JJ. Andrographis paniculata: a review of its traditional uses, phytochemistry and pharmacology. Medicinal and Aromatic Plants. 2014;3:4.
14. PC Sharma MBYJD. Database on medicinal plants used in ayurveda. In: Database on Medicinal plants used in Ayurveda. 4th ed. CCRAS; 2002. p. 700.
15. Mishra K, Dash AP, Swain BK, Dey N. Anti-malarial activities of Andrographis paniculata and Hedyotis corymbosa extracts and their combination with curcumin. Malaria J 2009;8:1–9.
16. Dey Y, Kumari S, Ota S, Srikanth N. Phytopharmacological review of Andrographis paniculata (Burm. f) Wall. ex nees. Int J Nutr Pharmacol Neurol Dis 2013;3:3.
17. Okhuarobo A, Ehizogie Falodun J, Erharuyi O, Imieje V, Falodun A, Langer P. Harnessing the medicinal properties of Andrographis paniculata for diseases and beyond: a review of its phytochemistry and pharmacology. Asian Pacific J Trop Dis 2014;4:213–22.
18. Siddhartha S, Archana M, Jinu J, Pradeep M. Anthelmintic potential of Andrographis paniculata, Cajanus cajan and Silybum marianum. Pharmacognosy J 2009;1:243–5.
19. Zhang XF, Tan BK. Antihyperglycaemic and anti-oxidant properties of Andrographis paniculata in normal and diabetic rats. Clin Exp Pharmacol Physiol 2000;27:358–63.
20. Singh RP, Banerjee S, Ramesha Rao A. Modulatory influence of Andrographis paniculata on mouse hepatic and extrahepatic carcinogen metabolizing enzymes and antioxidant status. Phytother Res 2001;15:382–90.
21. Sheeja K, Shihab PK, Kuttan G. Antioxidant and anti-inflammatory activities of the plant Andrographis paniculata nees. Immunopharmacol Immunotoxicol 2006;28:129–40.
22. Chandra R, Kumarappan CT, Kumar J, Mandal SC. Antipyretic activity of JURU-01-A polyherbal formulation. Global J Pharmacol 2010;4:45–7.
23. Allan JJ, Pore MP, Deepak M, Murali B, Mayachari AS, Agarwal A. Reproductive and fertility effects of an extract of Andrographis paniculata in male wistar rats. Int J Toxicol 2009;28:308–17.
24. Nilavembu Kudineer (Nilavembu Kashayam) Benefits, Uses and Dosage. Ayur Times; 2016.
25. Andrographis: Uses, Side Effects, Interactions, Dosage, and Warning. WebMD; 2010.
26. Mohideen SS, Yamasaki Y, Omata Y, Tsuda L, Yoshiike Y. Nontoxic singlet oxygen generator as a therapeutic candidate for treating tauopathies. Sci Reports Nat Publishing Group 2015;5:1-14.
27. Prem Kumar N, Vijayan SK, Dharsana JN, Seena KX, Anjana AK. Comparing the effect of antidiabetic activity of Andrographis paniculata, Salacia reticulata and Ocimum sanctum by in-vitro screening. Asian J Pharm Clin Res 2012;5:146-9.
28. Tan M, Oyong G, Shen C, Ragasa C. Cytotoxic labdane diterpenoids from andrographis paniculata (BURM. F.) nees. AJPCR 2017;10:99-104.
29. Kalyana Sundaram I, Sarangi DD, Sundararajan V, George S, Sheik Mohideen S. Polyherbal formulation with anti-elastase and anti-oxidant properties for skin anti-aging. BMC Complement Altern Med 2018;18:33.
30. Banerjee S, Pandey S, Mukherjee P, Sayeed A, Pandurangi AV, George S, et al. Investigation of cytotoxicity induced by Nigella sativa and Azadirachta indica using MDA-MB-231, HCT 116 and SHSY5Y cell lines. Pharmacogn J 2017;9:192-5.
31. Hafid AF. The combination therapy model of Andrographis paniculata extract and chloroquine on Plasmodium berghei infected mice. AJPCR 2015;8:205-8.
32. NH, SN, BP, RH, TR. Herbal wealth for hepatotoxicity: a review. AJPCR 2015;8:3-9.
33. Putra AC, Hanafi M, Pan Y, Yanuar A. Andrographolide and its derivative-a story of antimalarial drug design and synthesis. Int J Appl Pharm 2017;9:98-101.
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How to Cite
Sahabudeen, S. M., H. Nag, S. Madhavan, A. Chatterjee, P. Dan, M. Bhattacharya, and Z. A. Nawaz. “FECUNDITY STUDIES OF ANDROGRAPHIS PANICULATA ON DROSOPHILA MELANOGASTER”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 6, Apr. 2019, pp. 23-27, doi:10.22159/ijpps.2019v11i6.32093.
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