GC-MS ANALYSIS, HPTLC FINGERPRINT PROFILE AND DPPH FREE RADICAL SCAVENGING ASSAY OF METHANOL EXTRACT OF MARTYNIA ANNUA LINN SEEDS

  • Mazahar N Farooqui Dr.Rafiq Zakaria College for Women, Aurangabad (M.S), India
  • ali alrabie Dr.Rafiq Zakaria College for Women, Aurangabad (M.S), India
  • Ola Basaꞌar Dr.Rafiq Zakaria College for Women, Aurangabad (M.S), India
  • Inas Al-Qadsy Dr.Rafiq Zakaria College for Women, Aurangabad (M.S), India

Abstract

Objective: To investigate the photochemical constituents present in methanol extract of martynia annua seeds using Gas Chromatography-Mass Spectroscopy(GC-MS), High-Performance Thin–Layer Chromatography(HPTLC) analysis and study antioxidant activity.


Methods: Methanol extract of Martynia annua seeds were subjected to GC-MS and HPTLC analysis. HPTLC analysis was carried out using GAMAG system with a linomate5 applicator, system mobile phase (Toluene: Chloroform: Ethanol (4:4:1 V/V/V)), two different volume of extract was applied 2 µl and 5 µl. GC-MS analysis was carried out on JEOL GC MATE ΙΙ, column HP 5 MS and Quadruple double focusing mass analyzer. Antioxidant activity was determined by DPPH assay.


Results: GC-MS analysis provided 17 peaks indicating the presence of seventeen different phytochemicals in methanol extract of martynia annua seeds. HPTLC fingerprint showed 6 peaks at both size 2 µl and 5 µl at 254 nm whereas 4 peaks, 9 peaks were detected at 366 nm for 2 µl and 5 µl respectively. After derivatization with 10 % methanolic sulphuric acid, 8 peaks, 11 peaks were detected for 2 µl and 5 µl respectively when the derivatized plate was scanned at 540 nm. DPPH free radical scavenging result showed EC50 value of 44.1±1.1 µg/ml.


Conclusion: The GC-MS analysis showed the presence of fatty acids, ester, aldehydes and ketones whereas in HPTLC different peaks at different UV-lights before and after derivatization were observed. Maximum percentage inhibition using DPPH assay was found 74 at concentration of 50 µg/ml.

Keywords: Martynia annua, GC-MS, HPTLC fingerprint, Antioxidant activity

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Farooqui, M. N., ali alrabie, O. Basaꞌar, and I. Al-Qadsy. “GC-MS ANALYSIS, HPTLC FINGERPRINT PROFILE AND DPPH FREE RADICAL SCAVENGING ASSAY OF METHANOL EXTRACT OF MARTYNIA ANNUA LINN SEEDS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 6, Apr. 2019, pp. 16-22, doi:10.22159/ijpps.2019v11i6.32206.
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