DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF GALLIC ACID, CURCUMIN AND PIPERINE IN AN AYURVEDIC FORMULATION

  • MEHJABEEN SHAIKH Department of Quality Assurance, Bharati Vidyapeeth's College of Pharmacy, Sector 8, C. B. D. Belapur, Navi Mumbai 400614
  • ARUNA P. JADHAV Department of Quality Assurance, Bharati Vidyapeeth's College of Pharmacy, Sector 8, C. B. D. Belapur, Navi Mumbai 400614

Abstract

Objective: The objective of the present work was to establish a simple, precise, accurate and robust method for simultaneous estimation of gallic acid, curcumin and piperine from the marketed ayurvedic formulation by liquid chromatography.


Methods: The separation was carried out on Hemochrom C18 Column (250 mm × 4.6 mm ID, 5 µm pore size) with a mobile phase methanol: acetonitrile: water (pH 3.2adjusted by using orthophosphate acid) in the ratio 70:20:10v/v by isocratic elution modeat 25 °C and the flow rate was setat0.8 ml/min. The analysis was carried out atisoabsorptive wavelength of 295 nm.


Results: The retention time of gallic acid, curcumin and piperine was found to be 3.3(±0.2), 4.7 (±0.2) and 5.6 (±0.2) min, respectively. The linearity range for gallic acid, curcumin and piperine was found to be 10-70 μg/ml, 20-80 µg/ml and 2-14 µg/ml, respectively with the coefficient of linear regression greater than 0.99 for all markers. Mean percent recoveries for gallic acid, curcumin, and piperine were found within the limit of acceptance (99-100%). The percent relative standard deviation (%RSD) for precision and robustness was found less than 2%, which indicates the method is precise and robust. The developed method applied for quantification of these markers from the marketed ayurvedic formulation of Dekofcyn tablet.


Conclusion: The developed method was found to be simple, rapid, precise and reproducible for standardization of Dekofcyn tablet and can be useful for other formulations containing these three markers.

Keywords: Reverse-phase high-performance liquid chromatography (RP-HPLC), Gallic acid, Curcumin, Piperine, Method validation, Ayurvedic formulation, Standardization

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References

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SHAIKH, M., and A. P. JADHAV. “DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF GALLIC ACID, CURCUMIN AND PIPERINE IN AN AYURVEDIC FORMULATION”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 6, May 2020, pp. 81-86, doi:10.22159/ijpps.2020v12i6.33801.
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