PHARMACOGNOSTICAL AND PHYTOCHEMICAL STANDARDIZATION OF PHYSALIS MINIMA L. LEAF

  • DIBYENDU SHIL Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Hathkhowapara, Guwahati 781017, India
  • DAMIKI LALOO Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Hathkhowapara, Guwahati 781017, India
  • SMRITI REKHA CHANDA DAS Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Hathkhowapara, Guwahati 781017, India
  • SUVAKANTA DASH Regional Institute of Pharmaceutical Science and Technology, Agartala 799006, India

Abstract

Objective: To evaluate the pharmacognostical and phytochemical parameters of Physalis minima leaf.


Methods: The leaf of Physalis minima was examined for macroscopical, microscopical, physicochemical parameters and fluorescence analysis. Extracts obtained from the leaf was analyzed for phytochemical screening and estimation of total tannin, phenolic and flavonoid content following the standard procedure available in the literature.


Results: Morphologically, the leaf was found to be ovate in shape, 5 to 8 cm long and up to 3 cm width with dentate margin, asymmetrical base, hairy surface, reticulate veins on each side of midrib, green in colour, characteristic odour and slight bitter in taste. Microscopic study has shown the important diagnostic characters of Solanaceae family which is characterized by presence of dorsiventral leaf with anomocytic stomata, grandular or uniseriate trichomes and cluster crystal of calcium oxalate. Physicochemical parameters like foreign organic matter (0.78%), loss on drying (8.23%), total ash (11.4%), acid insoluble ash (2.2%), water-soluble ash (6.1%), sulphated ash (2.4%) alcohol soluble extractive (10.4%), water-soluble extractive (9.5%), ether soluble extractive (1.8%) foaming index (below 100), swelling index (1), volatile oil and heavy metal content were quantified. Phytochemical analysis of different extracts of Physalis minima leaf has shown the presence of phytoconstituents viz. alkaloids, steroids, tannin, flavonoids, protein. Quantification of phytoconstituents was also reported like phenols (10.59±0.65 mg/gm equivalent to tannic acid), tannin (8.24±0.27 mg/gm equivalent to tannic acid) and flavonoids (87.17±0.87 mg/gm equivalent to rutin) respectively.


Conclusion: This present study was provided the qualitative and quantitative standard of Physalis minima will help to prevent the possible steps of adulteration with other species of the same genus.

Keywords: Physalis minima, Leaf, Standardization, Pharmacognostical, Physicochemical, Phytochemical

Downloads

Download data is not yet available.

Author Biographies

DIBYENDU SHIL, Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Hathkhowapara, Guwahati 781017, India

Pharmacognosy

DAMIKI LALOO, Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Hathkhowapara, Guwahati 781017, India

Assoc. Prof., Dept. of Pharmacognosy.

SMRITI REKHA CHANDA DAS, Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Hathkhowapara, Guwahati 781017, India

Asst. Prof., Cum HOD, Dept. of Chemistry.

SUVAKANTA DASH, Regional Institute of Pharmaceutical Science and Technology, Agartala 799006, India

Principal, Dept. of Phamaceutics.

References

1. Prasad SK, Sahu AN, Hemalatha S. Cytomorphological and physicochemical evaluation Cryptocoryne spiralis (Retzius) wydler. J Herbs Spices Med Plants 2012;18:304-7.
2. Supriya SK, Rohit AG, Rajashekhar N. Identity of ?ankari (Physalis Minima Linn.) in ayurvedic classics: a literature review. Anc Sci Life 2016;36 Suppl 1:6–11.
3. Fasihuddin BA, Ghazally I. Medicinal plants used by kadazandusun communities around crocker range. ASEAN Rev Biodiversity Environ Conservation 2003;7:1-10.
4. Karpagasundari C, Kulothungan S. Free radical scavenging activity of Physalis minima linn leaf extract (PMLE). J Med Plant Stud 2014;2 Suppl 4:59-64.
5. Jyothibasu T, Venkata Ramana K, Sreenu T, Ch Narasimha Raju Bh. Diuretic activity of methanolic extract of Physalis minima leaves. Scholars Research Library. Der Pharm Lett 2012;4 Suppl 6:1832-4.
6. Patel T, Shah K, Jiwan K, Shrivastava N. Study on the antibacterial potential of Physalis Minima linn. Indian J Pharm Sci 2011;73 Suppl 1:111–5.
7. Karpagasundari C, Kulothunga S. Analysis of bioactive compounds in Physalis minima leaves using GC MS, HPLC, UV-VIS and FTIR techniques. J Pharmacogn Phytochem 2014;3 Suppl 4:196-201.
8. Khandelwal KR. Practical pharmacognosy, techniques and experiment. 22nd ed. Pune: Nirali Prakashan; 2012.
9. Khare P, Kishore K, Sharma DK. A study on the standardization parameters of Bauhinia variegate. Asian J Pharm Clin Res 2017;10:133-6.
10. Johansen DA. Plant micro-technique. New York: McGraw Hill; 1940.
11. Quality control methods for herbal materials. World health Organization. Geneva: Switzerland; 2011.
12. Chase CR, Pratt R. Fluorescence of powdered vegetable drugs with particular reference to the development of a system of identification. J Am Pharm Assoc 1949;38 Suppl 6:324-31.
13. Dibyendu S, Suvakanta D, Damiki L. A comprehensive quality control standardization of Mirabilis jalapa L. tuberous root. Int J Res Pharm Sci 2017;8 Suppl 3:336-41.
14. Khare P, Kishore K, Sharma DK. A study on the standardization parameters of Bauhinia variegate. Asian J Pharm Clin Res 2017;10:133-6.
15. Harborne JB. The flavonoids: advances in research. Chapman and Hall. London J Sci Food Agric 1973;3:90-103.
16. Rawat P, Bachheti RK, Kumar N, Rai N. Phytochemical analysis and evaluation of in vitro immunomodulatory activity of Rhododendron arboreum leaves. Asian J Pharm Clin Res 2018;11:123-8.
17. Kumaran A, Karunakaran J. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. Food Sci Technol 2007;40 Suppl 2:344–52.
18. Makkar HPS. Quantification of tannins in tree foliage: A laboratory manual for the FAO/IAEA coordinated research project on the use of nuclear and related techniques to develop simple tannin assays for predicting and improving the safety and efficiency of feeding ruminants of tanniniferous tree foliage, working document. Vienna: FAO/IAEA; 2000.
19. Traditional medicine strategy. World Health Organisation. Geneva: Switzerland; 2002-2005.
20. Metcalfe CR, Chalk L. Anatomy of dicotyledons. Vol II. Oxford, England: Clarendon Press; 1950.
21. Ahuja J, Suresh J, Deep A, Madhuri, Pratyusha, Ravi. Phytochemical screening of Aerial part of Artemisia parviflora Roxb: a medicinal plant. Der Pharm Lett 2011;3:116-24.
22. Sonia NS, Jessykutty PC. Drying technologies for good quality crude drug production of medicinal plants-a review. Int J Med Pharm Sci 2016;6:59-72.
23. Kala C, Ali SS, Chaudhary S. Comparative pharmacognostical evaluation of Costus speciosus (Wild Ginger) and Zingiber officinale (Ginger) rhizome. Int J Curr Pharm Res 2016;8:19-23.
24. Ruwali P, Ambwani TK, Gautam P, Thapliyal A. Qualitative and quantitative phytochemical analysis of Artemisia indica willd. J Chem Pharm Res 2015;7:942-9.
Statistics
123 Views | 101 Downloads
Citatons
How to Cite
SHIL, D., D. LALOO, S. R. CHANDA DAS, and S. DASH. “PHARMACOGNOSTICAL AND PHYTOCHEMICAL STANDARDIZATION OF PHYSALIS MINIMA L. LEAF”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 11, Nov. 2019, pp. 20-25, doi:10.22159/ijpps.2019v11i11.34995.
Section
Original Article(s)