• ZAM WISSAM Department of Analytical and Food Chemistry, Faculty of Pharmacy, Al-Andalus University for Medical Sciences, Tartous, Syrian Arab Republic


Objective: Pistachio (Pistacia vera L.) hull are usually discarded as waste, which can lead to environmental pollution even a significant portion of polyphenols are often present in high concentrations in the outer parts of fruits. In this way, using a pistachio hull as a source of bioactive compounds will increase the value of pistachio production and offer valorization for a useless by-product.

Methods: Different ethanol concentrations (5, 10, 25, 50, 75 and 95 %) were investigated and the extraction efficiency at extraction temperature from 20 to 90 °C and extraction times from 5 to 45 min was studied. The extraction yield of total polyphenols and proanthocyanidins from the pistachio hull and the antioxidant activity of the extract were evaluated.

Results: The obtained results indicated relationships between the tested parameters and extraction yield. The maximum yield for total polyphenols and proanthocyanidins was obtained with 25% ethanol at 60 °C for 15 min (13.91±0.72 and 5.86±0.45 g/100g dry weight, respectively). DPPH radical scavenging activities of extracts were proved to have a linear relationship with the polyphenols yield in the extracts (R2=0.9907) with a maximum DPPH radical scavenging activity of 60.25%.

Conclusion: These findings propose that pistachio hull extracts can be a valuable source of bioactive compounds.

Keywords: Pistacia vera, Hulls, Polyphenols, Proanthocyanidins, Extraction, Solvent, Temperature, Time, DPPH


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1. Ozbek HN, Halahlih F, Gogu? F, Yan?k DK, Azaizeh H. Pistachio (Pistacia vera L.) hull as a potential source of phenolic compounds: evaluation of ethanol-water binary solvent extraction on antioxidant activity and phenolic content of pistachio hull extracts. Waste Biomass Valori; 2018. p. 1-10.
2. Paliwal R, Sharma V, Pracheta, Sharma S. Elucidation of free radical scavenging and antioxidant activity of aqueous and hydro-ethanolic extracts of Moringa oleifera pods. Res J Pharm Tech 2011;4:566-71.
3. Rosello Soto E, Galanakis CM, Brncic M, Orliend V, Trujilloe FJ, Mawsonf R, et al. Clean recovery of antioxidant compounds from plant foods, byproducts and algae assisted by ultrasounds processing. Modeling approaches to optimize processing conditions. Trends Food Sci Technol 2015;42:134-49.
4. Zam W, Bashour G, Abdelwahed W, Khayata W. Simple and fast method for the extraction of polyphenol and the separation of proanthocyanidins from carob pods. Scholars Acad J Pharm 2013;2:375-80.
5. Jyothi B, Pratap GP, Husain MK, Kazmi MH, Prasad GP, Sudarsanam G. Scientific validation and standardization of parpataka an ayurvedic drug with respect to fumaria indica. Int J Curr Pharm Res 2019;11:134-41.
6. Ravindran NT, Mohamed Sadiq A. Pharmacological activity of Ulva lactuca polyphenols fraction: cardioprotective and antioxidant activities against isoproterenol-induced myocardial infarction in rats. Asian J Pharm Clin Res 2019;12:133-6.
7. Tumbas Saponjac V, Canadanovic Brunet J, Cetkovic G, Jakisic M, Djilas S, Vulic J, et al. Encapsulation of Beetroot pomace extract: RSM optimization, storage and gastrointestinal stability. Molecules 2016;21:584-99.
8. Hintz T, Matthews KK, Di R. The use of plant antimicrobial compounds for food preservation. BioMed Res Int 2015:1-12. http://dx.doi.org/10.1155/2015/246264
9. Saitta M, Giuffrida D, La Torre GL, Potortì AG, Dugo G. Characterisation of alkylphenols in pistachio (Pistacia vera L.) kernels. Food Chem 2009;117:451-5.
10. Souri E, Amin G, Dehmobed Sharifabadi A, Nazifi A, Farsam H. Antioxidative activity of sixty plants from Iran. Iranian J Pharm Res 2004;3:55-9.
11. Hosseinzadeh H, Behravan E, Soleimani MM. Antinociceptive and anti-inflammatory effects of Pistacia vera leaf extract in mice. Iranian J Pharm Res 2011;10:821-5.
12. Rajaei A, Barzegar M, Mobarez AM, Sahari MA, Esfahani ZH. Antioxidant, anti-microbial and antimutagenicity activities of pistachio (Pistachia vera) green hull extract. Food Chem Toxicol 2010;48:107-12.
13. Taghizadeh SF, Davarynejad G, Asili J, Nemati SH, Karimi G. Assessment of phenolic profile and antioxidant power of five pistachio (Pistacia vera L.) cultivars collected from four geographical regions of Iran. Avicenna J Phytomed 2018;8:33-42.
14. Ozturk I, Ekici L, Yetim H, Sagd?c O. Antioxidative, antiradikale und antimikrobielle aktivitaten des fruchthullen-extrakts von frischen antep-pistazien. J Verbrauch Lebensm 2010;5:163-7.
15. Vahabzadeh F, Mehranian M, Mofarrah E. Antioxidant activity of pistachio hulls. J Am Oil Chem Soc 2004;81:621-2.
16. Zam W, Bashour G, Abdelwahed W, Khayata W. Effective extraction of polyphenols and proanthocyanidins from pomegranate’s peel. Int J Pharm Pharm Sci 2012;4:675-82.
17. Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients 2010;2:1231-46.
18. Waterhouse AL. Determination of total phenolics. In: Wrolstad RE, (Ed.), Current protocols in food analytical chemistry. Hoboken NJ: John Wiley and Sons. 2001. p. 1111-8.
19. Porter LJ, Histich LN, Chan BG. The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry 1986;25:223-30.
20. Brand Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol; 1995. p. 25-30.
21. Sajeeda N, Kolgi RR, Shivakumara SL, Shivaraj Y, Karigar CS. Comparative phytochemical profile and antioxidant property of bark, flowers and leaves extracts of Simarouba glauca. Asian J Pharm Clin Res 2019;12:56-63.
22. Zam W, Al Asaad N, Jarkas B, Nukari Z, Dounia S. Extracting and studying the antioxidant capacity of polyphenols in dry linden leaves (Tilia Cordata). J Pharmacogn Phytochem 2017;6:258-62.
23. Brglez Mojzer E, Knez Hrncic M, Skerget M, Knez Z, Bren U. Polyphenols: Extraction methods, antioxidative action, bioavailability and anticarcinogenic effects. Molecules 2016;21:E901.
24. Spigno G, Tramelli L, De Faveri DM. Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. J Food Eng 2007;81:200-8.
25. Spirad G, Prakash V, Narasinga MS. Extractability of polyphenols of sunflower seed in various solvents. J Biosci 1982;4:145-52.
26. Bucic Kojic A, Planinic M, Tomas S, Jakobek L, Seruga M. Influence of solvent and temperature on extraction of phenolic compounds from grape seed, antioxidant activity and colour of extract. Int J Food Sci Technol 2009;44:2394-401
27. Sulaiman ISC, Basri M, Masoumi HRF, Chee WJ, Ashari SE, Ismail M. Effects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology. Chem Cent J 2017;11:54.
28. Shi J, Yu J, Pohorly J, Young JC, Bryan M, Wu Y, et al. Optimization of the extraction of polyphenols from grape seed meal by aqueous ethanol solution. J Food Agric Environ 2003;1:42-7.
29. Naczk M, Shahidi F. Extraction and analysis of phenolics in food. J Chromatogr A 2004;1054:95-111.
30. Vergara Salinas JR, Perez Jimenez J, Torres JL, Agosin E, Perez Correa JR. Effects of temperature and time on polyphenolic content and antioxidant activity in the pressurized hot water extraction of deodorized thyme (Thymus vulgaris). J Agric Food Chem 2012;60:10920-9.
31. Horzic D, Jambrak AR, Belscak Cvitanovic A, Komes D, Lelas V. Comparison of conventional and ultrasound-assisted extraction techniques of yellow tea and bioactive composition of obtained extracts. Food Bioprocess Tech 2012;5:2858-70.
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How to Cite
WISSAM, Z. “OPTIMIZATION OF DIFFERENT PARAMETERS FOR THE EXTRACTION OF POLYPHENOLS AND PROANTHOCYANIDINS FROM PISTACIA VERA HULLS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 12, Dec. 2019, pp. 36-39, doi:10.22159/ijpps.2019v11i12.35556.
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