ANALYSIS OF DOXORUBICIN AND DOXORUBICINOL IN DRIED BLOOD SPOT OF BREAST CANCER PATIENTS FOR MONITORING THE CARDIOTOXICITY OF DOXORUBICIN

  • YAHDIANA HARAHAP Faculty of Pharmacy, Universitas Indonesia, Depok 16424, West Java, Indonesia
  • SABRINA NUR AMALIA Faculty of Pharmacy, Universitas Indonesia, Depok 16424, West Java, Indonesia
  • ALDHI ANARTA Faculty of Pharmacy, Universitas Indonesia, Depok 16424, West Java, Indonesia
  • RAMADHAN Dharmais Cancer Hospital, Jakarta, 11420, Indonesia

Abstract

Objective: This study aimed to analyze doxorubicin and doxorubicinol levels in Dried Blood Spot (DBS) from 25 breast cancer patients who received doxorubicin in their therapeutic regiment.


Methods: DBS samples were extracted by protein precipitation method and analyzed using Ultra Performance Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS), with the Acquity UPLC BEH C18 Waters chromatography column (2.1 x 100 mm x 1.7 μm). The mobile phase consisted of 0.1% acetic acid (eluent A) and acetonitrile (eluent B) with gradient elution; the flow rate was 0.15 ml/min and runtime, 7 min. This method was linear within the concentration range of 10–200 ng/ml for doxorubicin and 4–100 ng/ml for doxorubicinol.


Result: The analysis results showed that doxorubicin levels were in the range of 11.01 ng/ml to 93.75 ng/ml and doxorubicinol was 5.80 ng/ml to 58.57 ng/ml.


Conclusion: Cumulative doses of all patients were in the range of 49.11 mg/m2to 303.70 mg/m2, which have cardiomyopathy incidence rates<4%.

Keywords: Doxorubicin, Doxorubicinol, Dried blood spot, Breast cancer, LC-MS/MS

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HARAHAP, Y., S. N. AMALIA, A. ANARTA, and RAMADHAN. “ANALYSIS OF DOXORUBICIN AND DOXORUBICINOL IN DRIED BLOOD SPOT OF BREAST CANCER PATIENTS FOR MONITORING THE CARDIOTOXICITY OF DOXORUBICIN”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 4, Mar. 2020, pp. 21-25, doi:10.22159/ijpps.2020v12i4.36707.
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