• FARAH IBRAHIM Institut de Chimie Organique et Analytique, Universite d’Orleans, CNRS FR 2708, UMR7311, Orleans, France
  • ALI JABER Laboratoire de Recherche et Developpement des Medicaments et des Produits Naturels (RDMPN), Faculty of Pharmacy, Lebanese University, Beirut, Lebanon
  • GHASSAN IBRAHIM Laboratoire de Recherche et Developpement des Medicaments et des Produits Naturels (RDMPN), Faculty of Pharmacy, Lebanese University, Beirut, Lebanon
  • EDMOND CHEBLE Laboratoire de Recherche et Developpement des Medicaments et des Produits Naturels (RDMPN), Faculty of Pharmacy, Lebanese University, Beirut, Lebanon



Annona squamosa, Antioxidant, DPPH, Phytochemical screening, Total phenol content


Objective: The aim of this work was to characterize the antioxidant properties and to evaluate the total phenol content of leaves, bark, pericarp, and pulp extracts of Lebanese Annona squamosa Linn. (A. squamosa),, as well as a total screening of secondary metabolites present in the various plant parts studied.

Methods: Two solvent systems were used for extraction: ethanol 80 % and methanol 80 %. The antioxidant activity of different extracts was investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The Total Phenol Content (TPC) of the different plant parts are determined and compared via Folin-Ciocalteu method. The results were presented as the mean of three separate experiments and error bars were used to illustrate standard deviation.

Results: The phenolic content was found to be highest in the A. squamosa leaves methanolic and ethanolic extracts (117.2 mg and 112.92 gallic acid extract/g, respectively). The results showed that A. squamosa leaves methanolic and ethanolic extracts display the highest antioxidant activities than the bark, pulp and pericarp extracts, with half-maximal inhibitory concentration values 13.61 and 15.97 μg. ml-1 respectively. Ethanol 80 % and methanol 80 % were found to be efficient for the extraction of phenolic compounds.

Conclusion: Results of this study indicate the presence of promising compounds in Lebanese A. squamosa that are able to act as antioxidants and free radical scavengers.


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Aldred EM. Pharmacology: a handbook for complementary healthcare professionals. 1st ed. Edinburgh: Churchill Livingstone; 2009.

Khanna RD, Karki K, Pande D, Negi R, Khanna RS. Inflammation, free radical damage, oxidative stress and cancer. Interdiscip J Micro inflammation 2014;1:109.

Kukreja RC, Hess ML. The oxygen free radical system: from equations through membrane-protein interactions to cardiovascular injury and protection. Cardiovasc Res 1992;26:641-55.

Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39:44-84.

Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J 2012;5:9-19.

Sen S, Chakraborty R. The role of antioxidants in human health. In: Silvana A, Hepel M. editors. Oxidative stress: diagnostics, prevention, and therapy. Washington, DC: American Chemical Society; 2011. p. 1-37.

Pratt DE. Natural antioxidants from plant material. In: Huang MT, Ho CT, Lee CY. editors. Phenolic compounds in food and their effects on health II, Antioxidants and cancer prevention. Washington, DC: American Chemical Society; 1992. p. 54-71.

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010;4:118-26.

Agrawal M, Agrawal Y, Itankar P, Patil A, Kelkar A, Vyas J. Pharmacognostical evaluation of Annona squamosa linn. Int J Phytomed 2011;3:480-5.

Natural Resources Conservation Service (NRCS). Plants profile Annona squamosa L. united states department of agriculture; 2008. p. 4-17.

Yassine F, Sassine YN. State of annona cultivation in Lebanon. Master thesis, plant production, lebanese university, faculty of agriculture and veterinary sciences; 2014.

Gajalakshmi S, Divya R, Deepika VD, Mythili S, Sathiavelu A. Pharmacological activities of Annona squamosa: a review. Int J Pharm Sci Rev Res 2011;10:24–9.

Vikas B, Akhil BS, Remani P, Sujathan K. Free radical scavenging properties of Annona squamosa. Asian Pac J Cancer Prev 2017;18:2725–32.

Zahid M, Arif M, Rahman MA, Mujahid M. Hepatoprotective and antioxidant activities of Annona squamosa seed extract against alcohol-induced liver injury in sprague dawley rats. Drug Chem Toxicol 2018;9:1-7.

Salman HA, Senthilkumar R. Antibacterial activity of annona squamosa l. and annona reticulata l. against clinical isolates of mutans streptococci the causative agents of dental caries. Asian J Pharm Clin Res 2015;8 Suppl 4:152-5.

Elumalai N, Parameswaran I, Panchanatham ST. Anticancer effect of methanolic extract of annona squamosa on mammary carcinoma in rats with reference to glycoprotein components, lysosomal, and marker enzymes. Asian J Pharm Clin Res 2017;10 Suppl 9:180-5.

Chakraverty R, Bhattacharya A. The pharmacological properties of Annona squamosa linn: a review. IJPE 2016;4:692-9.

Kachhawah JBS, Sharma N, Tyagi S, Sharma KK. Screening of stem bark methanol extract of Annona squamosa for antibacterial activity. Int J Curr Pharm Res 2012;4:48-50.

Gamble JS. Flora of the presidency of madras. London: West, Newman and Adlard; 1921.

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.

Kumawat BK, Gupta M, Singh T, Singh Y. Free radical scavenging effect of various extracts of leaves of Balanites aegyptiaca (L.) delile by DPPH method. Asian J Plant Sci Res 2012;2:323-9.

El-Chaghaby GA, Ahmad AF, Ramis ES. Evaluation of the antioxidant and antibacterial properties of various solvents extracts of Annona squamosa L. leaves. Arabian J Chem 2014;7:227-33.

Sabbah A, Nasser M, Hijazi A, Rammal A, Nasser G. Phytochemical screening and cytotoxic activity of two extracts from seeds of Lebanese Annona squamosa L. Int J Pharma Res Health Sci 2017;5:1586-91.

Mahawar V, Patidar K, Joshi N. Development and evaluation of an herbal antiaging cream formulation containing Annona squamosa leaf extract. Asian J Pharm Clin Res 2019;12:210-4.

Kaurinovic B, Vastag D. Flavonoids and phenolic acids as potential natural antioxidants. In: Antioxidants; Shalaby E. editors. IntechOpen: London, UK; 2019.

Fernandez IM, Chagas EA, Filho AAM, Saravia SAM, Carvalho Dos Santos R, Cardoso PC, et al. Evaluation of total phenolic compounds and antioxidant activity in amazon fruit. Chem Eng Trans 2018;64:649-54.

Mariod AA, Abdelwahab SI, Elkheir S, Ahmed JM, Fauzi PNM, Chuen ChS. Antioxidant activity of different parts from Annona squamosa, and Catunaregam nilotica methanolic extract. Acta Sci Pol Technol Aliment 2012;11 Suppl 3:249-57.

Leong LP, Shui G. An investigation of antioxidant capacity of fruits in singapore markets. Food Chem 2002;76:69–75.

Jayaprakasha GK, Patil BS. In vitro evaluation of the antioxidant activities in fruit extracts form citron and blood orange. Food Chem 2007;101:410–8.

Cai YZ, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 2004;74:2157-84.

Liu HY, Qiu NX, Ding HH, Yao RQ. Polyphenols content and antioxidant capacity of 68 Chinese herbals suitable for medical or food uses. Food Res Int 2008;41:363-70.

Salas Coronado R, Villanueva Canongo C, Hernandez Carlos B. Antioxidant compounds and their antioxidant mechanism. In: Antioxidants, Shalaby E. Ed. IntechOpen: London, UK; 2019.



How to Cite

IBRAHIM, F., A. JABER, G. IBRAHIM, and E. CHEBLE. “ANTIOXIDANT ACTIVITY AND TOTAL PHENOL CONTENT OF DIFFERENT PLANT PARTS OF LEBANESE ANNONA SQUAMOSA LINN”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 12, no. 8, Aug. 2020, pp. 100-5, doi:10.22159/ijpps.2020v12i8.36992.



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