FORMULATION AND CHARACTERIZATION OF SOLID LIPID NANOPARTICLES CONTAINING GINGER OIL FOR ENHANCEMENT OF STABILITY

  • PRIYANKA SAWANT Poona College of Pharmacy, Pune, Maharashtra, India
  • POONAM KAREKAR Indira College of Pharmacy, Pune
  • KARISHMA WAGHMARE D. D. Vispute College of Pharmacy and Research Center, New Panvel, Navi Mumbai Maharashtra, India

Abstract

Objective: To develop and characterize ginger oil loaded solid lipid nanoparticles (SLN) for enhancement of its stability.


Methods: Ginger oil loaded SLNs were prepared in four different batches by double emulsification method using different concentrations of soya lecithin and Tween 80. Further, these batches were characterized for particle size, zeta potential, drug entrapment efficiency and in vitro release study. After observing the results, batch F4 was further characterized by Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Spectroscopy (TEM) and Differential Scanning Calorimetry (DSC). In addition the optimized batch was subjected to anti-microbial study. Finally, stability studies were done by storing the F4 formulation at accelerated condition, room temperature, refrigerated temperature and photostability were performed by exposing the formulation to UV/fluorescence lamp for 6 mo.


Results: The encapsulation efficiency of various batches of SLNs was in the range of 79.75 to 90.24%. The size ranges varied between 50 to 1000 nm. Zeta potential of all formulations was found to be in the range of-44.52 to-49.37 mV. The FTIR spectra of optimized F4 batch indicated no significant structural changes or complexation reactions between drug and excipients. Moreover, TEM image of displayed spherical shape with smooth surface. In vitro drug release study exhibited 95% drug release up to 12 h which indicated suitability of formulation. Thus F4 batch formulation stored at room temperature and refrigerated conditions was found most stable while, accelerated and photostability samples were found to be most susceptible in comparison.


Conclusion: The physicochemical stability of ginger oil extract was enhanced by loading it into solid lipid nanocarriers; the resulting SLNs also showed good antimicrobial potential against Klebsiella pneumonia throughout storage conditions.

Keywords: Solid lipid nanoparticles, Ginger oil, Stability study, Antimicrobial study

Downloads

Download data is not yet available.

References

1. Butt MS, Sultan T. Ginger and its health claims: molecular aspects. Crit Rev Food Sci Nutr 2011;51:383-93.
2. Bent S. Herbal medicine in the United States: a review of efficacy, safety and regulation. J Gen Intern Med 2008;23:854-9.
3. Ahmad A, Abbas F, A lkarkhi M, Hena S, Mobin B, Khoo S, et al. Optimization of soxhlet extraction of herba Leonuri using factorial design of an experiment. Int J Chem 2010;2:198-205.
4. Ali BH. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chem Toxicol 2008;46:409-20.
5. Grzanna R, Lindmark L, Frondoza CG. Ginger–an herbal medicinal product with broad anti-inflammatory actions. J Med Food 2005;8:125-32.
6. Phillips S, Ruggier R, Hutchinson SE. Zingiber officinale (ginger)–an antiemetic for day case surgery. Anaesthesia 1993;48:715-7.
7. Fischer Rasmussen W, Kjaer SK, Dahl C, Asping U. Ginger treatment of hyperemesis gravidarum. Eur J Obstet Gynecol Reprod Biol 1990;38:19-24.
8. Stewart JJ, Wood MJ, Wood CD, Mims ME. Effects of ginger on motion sickness susceptibility and gastric function. Pharmacology 1991;42:111-20.
9. Sharma SS, Kochupillai V, Gupta SK, Seth SD, Gupta YK. Antiemetic efficacy of ginger (Zingiber officinale) against cisplatin-induced emesis in dogs. J Ethnopharmacol 1997;57:93-6.
10. McGee, Harold. On food and cooking: the science and lore of the kitchen. Scribner 2004;2:425-6.
11. Jolad SD, Lantz RC, Solyon AM, Chen GJ, Bates RB, Timmermann. Fresh organically grown ginger (Zingiber officinale): composition and effects on LPS-induced PGE2 production. Phytochemistry 2004;65:1937-54.
12. Muller RH. Colloidal carriers for controlled drug delivery and targeting. boca raton, Fl: CRC Press; 1991. p. 371-3.
13. Muller RH, Mehnert W, Lucks JS, Schwarz C, Muhlen A, Weyers H, et al. Solid lipid nanoparticles (SLN)-an alternative colloidal carrier system for controlled drug delivery. Eur J Pharm Biopharm 1995;41:62–9.
14. Muller RH, Mader K, Gohla S. Solid lipid nanoparticles (SLN) for controlled drug delivery-are view of state of the art. Eur J Pharm Biopharm 2000;50:161–77.
15. Neeta Rai, Abhishek Kumar Jain, Jobin Abraham. Formulation and evaluation of herbal antidandruff shampoo containing garlic loaded solid lipid nanoparticles. Int J Pharm Res Rev 2013;2:12-24.
16. Westesen K. Novel lipid-based colloidal dispersions as potential drug administration systems expectations and reality. Colloid Polymer Sci 2000;278:608-18.
17. Vijayan, Shaik Aafreen, S Sakthivel, K Ravindra Reddy. Formulation and characterization of solid lipid nanoparticles loaded neem oil for topical treatment of acne. J Acute Disease 2013;13:282-6.
18. Trotta M, Cavalli R, Carlotti ME. Solid lipid micro particles were carrying Insulin formed by the solvent-in-water emulsion diffusion technique. Int J Pharm 2005;288:281-8.
19. Garg, S Singh. Enhancement in antifungal activity of eugenol in immunosuppressed rats through lipid nanocarriers. Colloids Surfaces B 2011;87:280–8.
20. Lakshmi Sirisha Kotikalapudi. Formulation and in vitro characterization of domperidone loaded solid lipid nanoparticles. Int J Pharm Biomed Res 2012;3:22-9.
21. Perez C, Pauli M, Bazerque P. An antibacterial assay by agar well diffusion method. Acta Bio Med Exp 1990;15:113-5.
22. S Singh, AK Dobhal, A Jain, JK Pandit, S Chakraborty. Formulation and evaluation of solid lipid nanoparticles of a water-soluble drug: zidovudine. Chem Pharm Bull 2010;58:650–5.
23. Suekawa M, Ishige A, Yuasa K, Sudo K, Aburada M, Hosoya E. Pharmacological studies on ginger. I. pharmacological actions of pungent constituents,-gingerol and-shogaol. J Pharmacobiodyn 1984;7:836-48.
24. T Helgason, TS Awad, K Kristbergsson, DJ McClements, J Weiss. Effect of surfactant surface coverage on the formation of solid lipid nanoparticles (SLN). J Colloid Interface Sci 2009;334:75–81.
25. JQ Zhang, J Liu, XL Li, BR Jast. Preparation and characterization of solid lipid nanoparticles containing silibinin. Drug Delivery 2007;14:381-7.
26. Taraka Sunil Kumar K, Mohan Varma M, Ravi Prakash. Development and optimization of enzalutamide-loaded solid lipid nanoparticles using box–behnken design. Asian J Pharm Clin Res 2019;12:67-76.
27. Anand Kumar Kushwaha, Parameswara Rao Vuddanda, Priyanka Karunanidhi, Sanjay Kumar Singh, Sanjay Singh. Development and evaluation of solid lipid nanoparticles of raloxifene hydrochloride for enhanced bioavailability. BioMed Res Int 2013;8:1-10.
28. Amir A Shaikh, Praveen D Chaudhari, Bhushan D Gavit. Effect of solubility enhancement and use of honey on anti-inflammatory and antibacterial activity of etodolac. Int J Green Pharm 2017;11:182-8.
29. Yiming Wanga, Jie Wanga, Zhenyu Yuana, Haoya Hana, Tao Lia, Li Lia, et al. Chitosan cross-linked poly (acrylicacid) hydrogels: drug release control and mechanism. Colloids Surf B 2017;152:252-9.
30. Mona Qushawy, Ali Nasr. Solid lipid nanoparticles (slns) as nano-drug delivery carriers: preparation, characterization and application. Int J Appl Pharm 2020;12:1-9.
Statistics
19 Views | 4 Downloads
Citatons
How to Cite
SAWANT, P., P. KAREKAR, and K. WAGHMARE. “FORMULATION AND CHARACTERIZATION OF SOLID LIPID NANOPARTICLES CONTAINING GINGER OIL FOR ENHANCEMENT OF STABILITY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 6, Apr. 2020, pp. 36-44, doi:10.22159/ijpps.2020v12i6.37357.
Section
Original Article(s)