antidiabetic activity EVALUATION OF ANTIDIABETIC ACTIVITY OF ZANTHOXYLUM OVALIFOLIUM LEAF EXTRACTS
EVALUATION OF ANTIDIABETIC ACTIVITY OF ZANTHOXYLUM OVALIFOLIUM LEAF EXTRACTS
Objective: The present study was aimed to investigate the antidiabetic activity of ethanolic and aqueous extract of Zanthoxylum ovalifolium on alloxan induced diabetic rat model in rats.
Materials and Methods: The Zanthoxylum ovalifolium leaves were evaluated for antidiabetic activity by using alloxan induced diabetic model in diabetic rats. Diabetes was induced by single intraperitoneal injection of alloxan (100 mg/kg) and rats were treated orally with test extracts, standard drug (glibenclamide 5mg/kg) and vehicle for 21 days. The hypoglycemic effects and lipid profile of diabetic rats were assessed. Finally, histopathological studies were carried out for pancreas.
Results: The acute toxicity studies revealed at the dose of 2000 mg/kg (b.w) of Zanthoxylum ovalifolium for ethanol and aqueous extract was found to be safe. Daily oral treatment with the test extracts and the standard drug for 21 days signiﬁcantly reduced blood glucose, total cholesterol triglyceride, high-density lipoprotein, low density lipoprotein and very low-density lipoproteins levels in time and dose dependent fashion when compare to control groups. Further histopathological studies also conﬁrmed regeneration of β-cells in the extract treated groups.
Conclusion: It can be concluded that ethanol and aqueous extract of Zanthoxylum ovalifolium at high dose (400 mg/kg) exhibited significant antihyperglycemic activity than extract at low dose (200 mg/kg) in alloxan induced diabetic rats. These extracts also showed improvement in parameters like lipid profile as well as regeneration β-cells in pancreas and so might be of value in diabetes treatment.
Keywords: Zanthoxylum ovalifolium, antidiabetic activity, lipid profile, alloxan and glibenclamide.
1. Mohan H. Textbook of pathology. 7th ed. Jaypee Brothers medical publishers, New Delhi; 2015. p. 808-09.
2. Imam K. Clinical features diagnostic criteria and pathogenesis of diabetes mellitus. Springer, New York; 2013. p. 340-355.
3. Elosta A, Ghous T, Ahmed N. Natural products as anti-glycation agents: possible therapeutic potential for diabetic complications. Current diabetes reviews. 2012; 8:92-108.
4. Patel DK, Prasad SK, Kumar R, Hemlatha S. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed 2012; 2:320-30.
5. International Diabetes Federation (IDF). Diabetes Atlas. 6th Ed. International Diabetes Federation (IDF); 2013.
6. Patel DK, Kumar R, Laloo D, Hemalatha S. Natural medicines from plant source used for therapy of diabetes mellitus: An overview of its pharmacological aspects. Asian Pac J Trop Dis 2012; 2:239-50.
7. Preetha PP, Devi VG, Rajamohan T. E?ects of coconut water on carbohydrate metabolism and pancreatic pathology of alloxan induced diabetic rats. Eur J Integr Med 2013; 5:234-40.
8. Ashok Kumar BS, Lakshman K, Jayaveea KN, Sheshadri Shekar D, Khan S, Thippeswamy BS, et al. Antidiabetic, antihyperlipidemic and antioxidant activities of methanolic extract of Amaranthus viridis Linn in alloxan induced diabetic rats. Exp Toxicol Pathol 2012; 64:75-9.
9. Negi JS, Bisht VK, Bh AK, Singh P, Sundriyal RC. Chemical constituents and biological activities of the genus Zanthoxylum: a review. African Journal of Pure and Applied Chemistry. 2011; 5:412-16.
10. Medhi K, Deka M, Bhau BS. The Genus Zanthoxylum–A Stockpile of Biological and Ethnomedicinal Properties. Open Access Scientific Reports. 2013; 2:697-704.
11. Khandelwal KR. Practical Pharmacognosy. 1st ed. Nirali publications, Pune; 1995. P.140-43.
12. Kokate CK, Purohit AP, Gokhale SB. Practical Pharmacognosy. 4th ed. Nirali Publications, Pune; 2005. p. 108-11.
13. OECD Guidelines for the testing of chemicals, Acute oral Toxicity-Up-and-Down-Procedure (UDP), OECD/OCDE 425; 2008.
14. Parthiban P, Ravikumar J, Anjana A. Antidiabetic activity of kovai kizhangu chooranam in alloxan induced diabetic rats. Int. J. Life Sci Pharm. Res. 2012; 2:68-72.
15. Chaurasia S, Saxena RC, Chaurasia ID, Shrivastav R. Antidiabetic activity of Luffa aegyptica (Mill) in alloxan induced diabetic rats. J Chem Pharm Res. 2011; 3:522-25.
16. Tomoda M, Shimada K, Konno C, Hikino H. Structure of Panaxan B., A. Hypoglycaemic glycan of Panax ginseng roots. Phytochemistry 1985; 24:2431-3.
17. Khanna P, Jain SC. Hypoglycaemic activity of polypeptide-p from a plant source. J Nat Prod 1981; 44:648-55.
18. Reher, G, Slijepcevic M, Krans L. Hypoglycemic activity of triterpenes and tannins from Sarcopoterium spinosum and two Sanguisorba Species. Planta Med 1991; 57:A57-58.
19. Schimizu M, Ito T, Rshima S, Mayashi T, Arisawa MM, Kurokowa S, Ito HY. Inhibition of lens aldose reductase by ?avonoids. Phytochemistry 1984; 23:1885-8.
20. Shankar M and Suthakaran. Antidiabetic activity of hydroalcoholic extract of Eugenia Jambolana leaves in alloxan induced diabetic rats.Int J Pharm Pharm Sci 2014;6(10):138-40.
21. Ramesh C and Prameela Rani A. Invivo and in-vitro evaluation of Tephrosia Calophylla for antidiabetic properties. Int J Pharm Pharm Sci 2018; 10(6):138-44.
22. Samir Derouiche,Manel Azzi and Abir Hamida.Effect of extracts aqueous of Phragmites Australis on carbohydrate metabolism, some enzyme activities and pancreatic islet tissue in alloxan induced diabetic rats. Int J Pharm Pharm Sci 2017; 9(6):54-58.
23. Jelodar G, Mohsen M and Shahram S. Effect of walnut leaf, coriander and pomegranate on blood glucose and histopathology of pancreas of alloxan – induced diabetic rats, African J. Traditional, Complementary and Alternative Medicines.2003; 3: 299 – 305.
24. Grover JK, Vats V and Rathi SS.Antihyperglycemic effect of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. J Ethnopharmacol.2000; 73:461-70.
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