• ANDREANYTA MELIALA Department of Physiology, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Jalan Farmako Sekip Utara, Sleman, Yogyakarta 55281, Indonesia
  • YUSTINA ANDWI ARI SUMIWI Department of Histology, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Jalan Farmako Sekip Utara, Sleman, Yogyakarta 5528, Indonesia
  • PARAMITA NARWIDINA Clinical Nutrition Research Group, Yogyakarta 55133, Indonesia
  • SRI LESTARI SULISTYO RINI Department of Physiology, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Jalan Farmako Sekip Utara, Sleman, Yogyakarta 55281, Indonesia
  • WIDIASTUTI SETYANINGSIH Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jalan Flora, Bulaksumur, Sleman, Yogyakarta 55281, Indonesia



Banana peel, Antidiabetic, Antidepressive, Dietary fiber, Serotonin


Objective: This study aimed to evaluate the antidiabetic and antidepressant effects of banana peel flakes in streptozotocin-induced diabetic rats.

Methods: Twenty-five male Wistar rats were classified into five groups with different treatments. Groups I to IV were diabetic rats model groups that consumed only standard diet, standard diet containing 5%, 10%, and 20% of banana peel flakes, respectively. While group V was a healthy control group fed a standard diet. Immunohistochemistry staining was measured to examine serotonin expression in the colon and pancreas.

Results: The diabetic rats treated with 20% banana peel flakes had a lower blood glucose concentration (p<0.05) compared with diabetic control and showed a shorter duration of immobility time (p<0.05) than the healthy control. Additionally, compared with diabetic control, the diabetic rats treated with 5% banana peel flakes showed higher serotonin expression (p<0.05) in the colon. In contrast, serotonin expression in the pancreas did not show any significant difference (p>0.05).

Conclusion: The present study disclosed that the banana peel flakes provided an antidepressant effect in the diabetic rats model, which might occur through the mechanism of controlling blood glucose concentration.


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Anderson R, Freedland K, Clouse R. The prevalence of comorbid depression in adults with diabetes: a meta-analysis. Diabetes Care 2001;24:1069–78.

Ali N, Jyotsna V, Kumar N. Prevalence of depression among type 2 diabetes compared to healthy non-diabetic controls. J Assoc Physicians India 2013;61:619–21.

Roy T, Lloyd C. Epidemiology of depression and diabetes: a systematic review. J Affect Disord 2012;142:S8-21.

Oxenkrug GF. Metabolic syndrome, age-associated neuroendocrine disorders, and dysregulation of tryptophan-kynurenine metabolism. Ann NY Acad Sci 2010;1199:1–14.

Banerjee M, Saxena M. Interleukin-1 (IL-1) family of cytokines: role in type 2 diabetes. Clin Chim Acta 2012;413:1163–70.

Shajib MS, Baranov A, Khan WI. Diverse effects of gut-derived serotonin in intestinal inflammation. Chem Neurosc 2017;8:920–31.

Costedio MM, Hyman N, Mawe GM, et al. Serotonin and its role in the colonic function and in gastrointestinal disorders. Dis Colon Rectum 2014;50:376-88.

Waclawikov B, Aidy S El. Role of microbiota and tryptophan metabolites in the remote effect of intestinal inflammation on brain and depression. Pharmaceuticals 2018;25:1-17.

Badawy AA. Modulation of tryptophan and serotonin metabolism as a biochemical basis of the behavioral effects of use and withdrawal of androgenic-anabolic steroids and other image and performance-enhancing agents. Int J Tryptophan Res 2018;11:1–16.

Richards P, Pais R, Habib A. High-fat diet impairs the function of glucagon-like peptide-1, producing L-cells. Peptides 2016;77:21–7.

Keszthelyi D, Troost F, Jonkers D. Does acute tryptophan depletion affect peripheral serotonin metabolism in the intestine? Am J Clin Nutr 2012;95:603–8.

Dehhaghi M, Kazemi H, Panahi S. Microorganisms, tryptophan metabolism, and kynurenine pathway: a complex interconnected loop influencing human health status. Int J Tryptophan Res 2019;12:1–10.

Clarke G, Grenham SPS. The microbiome-gut-brain axis during early life regulates the hippocampal serotonergic system in a sex-dependent manner. Mol Psychiatry 2013;18:666-73.

Kwon YH, Wang H, Denou E. Modulation of gut microbiota composition by serotonin susceptibility to colitis. Cell Mol Gastroenterol Hepatol 2019;7:709–28.

Cani PD, Osto M, Geurts L. Involvement of gut microbiota in the development of low-grade inflammation and type 2 diabetes associated with obesity. Gut Microbes 2012;3:279–88.

Nagarajaiah SB, Prakash J. Chemical composition and antioxidant potential of peels from three varieties of banana. Asian J Food Agro-Industry 2011;4:31–46.

Lewis D, Shaw G. A natural flavonoid and synthetic analogues protect the gastric mucosa from aspirin-induced erosion. J Nutr Biochem 2001;12:95–100.

Kusuma SF, Mita SR, Firdayani. Study on the antibacterial activity of fruit extracts of klutuk banana (musa balbisiana colla) against Shigella dysenteriae atcc 13313. Asian J Pharm Clin Res 2017;10:220–3.

Someya S, Yoshiki Y, Okubo K. Antioxidant compounds from bananas (Musa cavendish). Food Chem 2002;79:351–4.

Gopalan G, Prabha B, Joe A. Screening of Musa balbisiana colla. Seeds for antidiabetic properties and isolation of apiforol, a potential lead, with antidiabetic activity. J Sci Food Agric 2018;99:1–31.

Borah M, Das S. Antidiabetic, antihyperlipidemic, and antioxidant activities of musa balbisiana colla. in type 1 diabetic rats. Indian J Pharmacol 2017;49:71–6.

Purabi D, Ananya K, Daisy S. A review on Musa Balbisiana colla. Int J Pharm Sci Invent 2018;7:14–7.

Official methods of analysis of the Association of Official Analytical Chemists. In: AOAC. Gaithersburg MD, USA; 2005. p. 18.

Alam S, Ehsan SD. Antidepressant-like activity of banana peel extracts in mice. Tan pei tee and halijah hassan. Am J Med 2011;2:59–64.

Chand P, Garg A, Singla V. Evaluation of an immunohistochemical profile of breast cancer for prognostics and therapeutic use. Niger J Surg 2018;24:100–6.

Navghare V, Shashikant D. Suppression of type-II diabetes with dyslipidemia and nephropathy by peels of Musa cavendish fruit. Ind J Clin Biochem 2016;31:380–9.

Saad EA, Hassanien MM, El-hagrasy MA, Radwan KH. Antidiabetic, hypolipidemic and antioxidant activities and protective effects of punica granatum peels powder against pancreatic and hepatic tissues injuries in streptozotocin-induced iddm in rats. Int J Pharm Pharm Sci 2015;7:397-402.

Andallu B, Varadacharyulu NC. Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats. Clin Chim Acta 2003;338:3–10.

Jain D, Bansal M, Dalvi R. Protective effect of diosmin against diabetic neuropathy in experimental rats. J Integr Med 2014;12:35–41.

Balbi MDA, Crivellenti LC, Cristina D. The relationship of flavonoid intake during pregnancy with excess body weight and gestational diabetes mellitus. Arch Endocrinol Metab 2019;63:241–9.

Singh A, Singh S. Dietary fiber content of Indian diets. Asian J Pharm Clin Res 2015;8:58–61.

Gestel G, Besançon P. Comparative evaluation of the effects of two differents forms of dietary fibre (Rice bran vs wheat bran) on rat colonic mucosa and fæcal micro-flora. Ann Nutr Metab 1994;38:249–56.

Vries J De, Birkett A, Hulshof T. Effects of cereal, fruit and vegetable fibers on human fecal weight and transit time: a comprehensive review of intervention trials. Nutrients 2016;8:1–10.

Everard A, Geurts L, Caesar R. Intestinal epithelial MyD88 is a sensor switching host metabolism towards obesity according to nutritional status. Nat Commun 2014;5:1-12.

Ferraris RP, Vinnakota RR. Intestinal nutrient transport in genetically obese mice. Am J Clin Nutr 1995;62:540–6.

Mao J, Hu X, Xiao Y, Yang C, Ding Y, Hou N, et al. Overnutrition stimulates intestinal epithelium proliferation through beta-catenin signaling in obese mice. Diabetes Metab Res Rev 2013;62:3736-46.

Mahore JG, Shirolkar SV. Investigation of the effect of ripening and processing on the prebiotic potential of banana. J Young Pharm 2018;10:10–4.

Topping DL, Clifton PM. Short-chain fatty acids and human colonic function: roles of resistant starch and non-starch polysaccharides. Physiol Rev 2001;81:1031-64.

Ble Castillo JL, Juarez Rojop IE, Tovilla Zarate CA. Acute consumption of resistant starch reduces food intake but has no effect on appetite ratings in healthy subjects. Nutrients 2017;9:1–12.

Bindels L, Walter J, Ramer Tait A. Resistant starches for the management of metabolic diseases. Curr Opin Clin Nutr Metab Care 2015;18:559–65.

Catena Dell’Osso M, Rotella F, Dell’Osso A. Inflammation, serotonin and major depression. Curr Drug Targets 2013;14:571–7.

Gal E, Sherman A. L-Kynurenine: its synthesis and possible regulatory function in brain. Neurochem Res 1980;5:223–9.

Gkogkolou P, Böhm M. Advanced glycation end products key players in skin aging? Dermatoendocrinol 2012;4:3:259–70.

Kamba A, Daimon M, Murakami H. Association between higher serum cortisol levels and decreased insulin secretion in a general population. PLoS One 2016;11:1–10.

Adedayo BC, Oboh G, Oyeleye SI. Antioxidant and antihyperglycemic properties of three banana cultivars (Musa spp.). Scientifica 2016:1–7. 2016/8391398.

Fidrianny I, Rizki Kiki R, Insanu M. In vitro antioxidant activities from various extracts of banana peels using abts, dpph assays and correlation with phenolic, flavonoid, carotenoid content. Int J Pharm Pharm Sci 2014;6:299–303.

Samad N, Ullah N, Ayaz MM. Banana fruit pulp and peel involved in antianxiety and antidepressant effects while invigorate memory performance in male mice: possible role of potential antioxidants. Pak J Pharm Sci 2017;30:989–95.

Debjit Bhowmik KP, Sampath Kumar, M Umadevi. Traditional and medicinal uses of banana. J Pharmacogn Phytochem 2012;1:51–63.

Martin AM, Young RL, Leong L. The diverse metabolic roles of peripheral serotonin. Endocrinology 2017;158:1049–63.

Crane JD, Palanivel R, Mottillo EP. Inhibiting peripheral serotonin synthesis reduces obesity and metabolic dysfunction by promoting brown adipose tissue thermogenesis. Nat Med 2014;21:1–9.

Bodinham C, Smith L, Thomas E. Efficacy of increased resistant starch consumption in human type 2 diabetes. Endocr Connect 2014;3:75–84.

Takahashi T, Yano M, Minami J. Sarpogrelate hydrochloride, a serotonin2A receptor antagonist, reduces albuminuria in diabetic patients with early-stage diabetic nephropathy. Diabetes Res Clin Prac 2002;58:123–9.

Malyszko J, T Urano, R Knofler. Daily variations of platelet aggregation in relation to blood and plasma serotonin in diabetes. Thromb Res 1994;75:569–76.

Adeghate E, Ponery AS, Pallot D. Distribution of serotonin and its effect on insulin and glucagon secretion in normal and diabetic pancreatic tissues in rat. Neuro Endocrinol Lett 1999;20:315–22.

Marco J, Hedo JA, Villanueva ML. Inhibition of glucagon release by serotonin in mouse pancreatic islets. Diabetologia 1977;13:585–8.

Rodriguez Ambriz, Agama Acevedo I, Etovar J. Characterization of a fiber-rich powder prepared by liquefaction of unripe banana flour. Food Chem 2008;4:1515–21.

Kim W, Egan JM. The role of incretins in glucose homeostasis and diabetes treatment. Pharmacol Rev 2008;60:470–512.

Ramracheya R, Chapman C, Chibalina M. GLP-1 suppresses glucagon secretion in human pancreatic alpha-cells by inhibition of P/Q-type Ca 2+channels. Physiol Rep 2018;6:1–17.



How to Cite

MELIALA, A., Y. A. A. SUMIWI, P. NARWIDINA, S. L. S. RINI, and W. SETYANINGSIH. “BANANA PEEL FLAKES ALLEVIATE BLOOD GLUCOSE AND STRESS IN A DOSE-DEPENDENT MANNER”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 12, no. 8, Aug. 2020, pp. 75-81, doi:10.22159/ijpps.2020v12i8.37659.



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