• S. PAVITHR Department of Botany, Bharathiar University, Coimbatore, Tamilnadu, India
  • T. SEKAR Department of Botany, Bharathiar University, Coimbatore, Tamilnadu, India


Objective: In the current research, to determine the stem extract of Meliosma simplicifolia (L.) for total phenol, tannin, total flavonoid, antioxidant activity, anti-inflammatory and identify the phytoconstituents utilizing GC-MS and FT-IR.

Methods: The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, superoxide radical (O2•) scavenging activity. The anti-inflammatory activity was evaluated using carrageenan and formalin-induced paw edema models using Wistar albino rats. The GC-MS and FT-IR analysis of the methanolic stem extract of M. simplicifolia was revealed the presence of phytochemicals.

Results: Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (39.83±3.62GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia stem for as followed the studies. In stem the methanol extract showed the highest DPPH scavenging activity (124.3µg/ml). The anti-inflammatory activity has shown in high doses of methanolic extract 250 mg/kg of significant value (p<0.05) inhibition of paw edema, on 6th hour, respectively. The FT-IR analysis has confirmed their characteristic peak values and functional groups.

Conclusion: M. simplicifolia has an effective of anti-inflammatory activity and constitutes a potential source for the development of new treatments.

Keywords: M. simplicifolia, Phytochemicals, Antioxidant, GC-MS, FT-IR, Carrageen an induced acute paw edema


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1. Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plant. Afr J Biotechnol 2005;4:685-68.
2. Arpan A Bhatt, Shweta Pandey, Brajesh Singh, Gauravi Vyas, Gyanendra Datta Shukla, Upasnalati. The role of yoga therapy in the management of bronchial asthma (Tamakashwasa). Asian J Pharm Clin Res 2019;12:27-33.
3. Duh PD, Tu YY, Yen GC. Antioxidants activity of aqueous extract of Harnjyur (Chrysanthemum morifolium Ramat). M. Lebensmwiss Technol. Elmastas 2005;32:269-77.
4. Lena G. Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in latin America. Bio Resour Technol 2010;10:4676-89.
5. Bahramikia, Seifollahand Ardestani, Amin, Yazdanparast, Razieh. Protective effect of four Iranian medicinal plants against free radical-mediated protein oxidation. Food Chem 2009;115:37-42.
6. Hagerman AE, KM Reidl, GA Jones. High molecular weight polyphenolics (tannins) as biological antioxidants. J Agric Food Chem 1998;46:1887-92.
7. Prashant Tiwari, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur, Harleen Kaur. Phytochemical screening and extraction: review. Int Pharm Sci 2011;1:98-106.
8. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of aphosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337–41.
9. Petlevski R, Flajs D, KaloCera Z, Koncic MZ. Composition and antioxidant activity of aqueous and ethanolic Pelargonium radula extracts. South Afr J Bot 2013;85:17-22.
10. Murugan R, Parimelazhagan T. Comparative evaluation of different extraction methods for antioxidant and anti-inflammatory properties from Osbeckiaparvifolia Arn. an in vitro approach. J King Saud Univ Sci 2014;26:267–75.
11. Zhishen J, Mengcheng T, Jianming W. Determination of flavonoids contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64:555-9.
12. Sasidharan S, Darah I, Mohd Jain Noordin MK. Free radical scavenging activity and total phenolic compounds of Gracilariachangii. Int J Nat Eng Sci 2007;1:115-7.
13. Prieto P, Pineda M. Spectrophotometric quantisation of antioxidant capacity through the formation of aphosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem Aguilar M 1999;269:337-41.
14. Charles A, Winter, Edwin A, Risley, George W. Carrageenin-induced edema in hind paw of the rat as an assay for antiinflammatory drugs. Exp Biol Med 1962;111:544-7.
15. Jeong SM, Kim SY, Kim DR, Nam KC, Ahn DU. Effect of heat treatment on the antioxidant activity of extracts from citrus peels. J Agric Food Chem Lee SC 2004;52:3389-93.
16. Obrenovich ME, Li Y, Parvathaneni K, Yendluri BB, Palacios HH. Antioxidants in health, disease and aging. CNS Neurol. Disord Drug Targets G 2011;10:192-207.
17. Lunardelli A, Leite CE, Pires MGS. Extract of the bristles of Dirphia sp. increases nitric oxide in a rat pleurisy model. Inflammation Res 2006;55:129-35.
18. Suffredini AF, Fantuzzi G, Badolato R, Oppenheim J, O’Grady N. New insights into the biology of the acute phase response. J Clin Immunol 1999;19:203–14.
19. Jackson ML. Soil chemical analysis. Prentice Hall, New Delhi. Kinsella JE, Frankel E. et al. Possible mechanism for the protective role of the antioxidant in wine and plant foods. Food Technol 1993;47:85-9.
20. Kumar A, Bello B, Reichert H. Lineage-specific cell death in postembryonic brain development of Drosophila. Development 2009;136:3433-42.
21. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolisbytwocomplementary colorimetric methods. J Food Drug 2002;10:178-82.
22. Jose Roberto Medina Medrano, Norma Almaraz Abraca, Socorro Gonzalez Elizondo M, Jose Natividad Uribe Soto. Phenolic constituents and antioxidant properties of five wild species of Physalis (Solanaceae). Botany Study 2015;56:24-5.
23. Chen YK, Li XS, Yang GY, Chen ZY, Hu QF. Phenolic compounds from Nicotianatabacum and their biological activities. J Asian Nat Products Res 2012;14:450-6.
24. MA Mahat, BM Patil. Evaluation of anti-inflammatory activity of methanol extract of Phyllanthusamarusin experimental animal models. Indian J Pharm Sci 2007;69:33–6.
25. Wills AL. Release of histamine, kinin and prostaglandins during carrageenin induced inflammation of the rats. In: Prostaglandins, peptides and amines. Montagazza P, Horton EW. Eds. Academic Press: London UK; 1969;1:31-48.
26. Sosa S, Balick MJ, Arvigo R. Screening of the topical anti-inflammatory activity of some Central American plants. J Ethnopharmacol 2002;81:211–5.
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How to Cite
PAVITHR, S., and T. SEKAR. “PHYTOCHEMICAL PROFILING, FREE-RADICAL SCAVENGING AND ANTI-INFLAMMATORY ACTIVITIES OF MELIOSMA SIMPLICIFOLIA (L.)”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 8, June 2020, pp. 62-68, doi:10.22159/ijpps.2020v12i8.37936.
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