PHYTOCHEMIMCAL ANALYSIS OF WRIGHTIA TINCTORIA BARK EXTRACT IN WATER USING GC-MS
Keywords:Wrightia tinctoria, Phytochemical, GC-MS analysis, Apocyanaceae
Objective: In the present study, phytochemical constituents of crude water extract of Wrightia tinctoria (WT) bark is done using GC-MS technique. Wrightia tinctoria is an important medicinal herb being used in tribal areas of Chhattisgarh since long but chemical constituents of its bark responsible for the activities are still not studied in depth.
Methods: Dried bark powder was successively extracted with petroleum ether, ethyl acetate and methanol using soxhlet apparatus and lastly material was dissolved in distilled water for 10 hrs for extraction. Water extract was selected for the further analysis using Agilent 7890A GC with 5975MS.
Results: As per the GC-MS analysis, twelve different compounds namely benzene 1, 2, 4, 5-tetramethyl (2.85%), benzene 1, 2, 3, 5-tetramethyl (1.16%), 1-decanol, 2, 2-dimethyl (4.38%), phenol 2, 4-bis (1, 1-dimethyl ethyl) (7.78%), heptadecane (3.60%), 3-hexadecanol (3.30%), i-propyl tetradecanol (3.64%), benzo (h) quinoline (3.66%), n-hexadecanoic acid (6.54%), octadecanoic acid methyl ester (0.81%), phytane (1.95%) and pentadecane (2.25%) were characterized. Analysis and identification of presence of the compound in these extracts were done using the database of NIST library.
Conclusion: Study confirms the presence of biologically active phyto-constituent in water extract of Wrightia tinctoria bark those may be the key chemical of natural origin in new drugs designing against major disease those are being treated in tribal areas using this plant bark. Further confirmation of in vitro bioactivity using cell line culture is required which is planned as the future prospect of current study.
Peach K, Tracey MV. Modern methods of plant analysis. 1st ed. Springer Varlag, Berlin; 1955.
Ertuk O, Kati Yayli N, Demirbag Z. Antimicrobial property of Silene mutifida (Adams) rohrb plant extract. Turk J Biol 2006;30 Suppl 1:17-21.
Kumar AR, Subburathinam KM, Prabaker GJ. Phytochemical screening of selected medicinal plants of asclepiadaceae. Asian J Microbiol Biotechnol Environ Sci 2007;9 Suppl1:177-80.
The Wealth of India, Raw Materials, national institute of science communication and information resources. New Delhi, India. CSIR; 1976.
Kiritikar KR, Basu BD. Indian medicinal plants. 2nd ed. University of Allahabad, India; 1991.
Ranchandra PM, Basheemiya GLD, Srimannarayana G. Occurance of oleanolic acid in the pods of Wrightia tinctoria R Br. J Nat Prod 1993;56:1811-2.
Muruganadam AV, Bhattacharya SK, Ghosal S. Indole and flavonoid constituents of Wrightia tinctoria, W. tomentosa and W. coccinea. Indian J Chem 2000;39:125-31.
Warrier PK, Nambiar VPK, Ramankutty C. Indian Medicinal Plants. Vol. 5. Orient Longman Ltd Madras; 1996.
Shah GL, Gopal GV. Ethnomedical notes from the tribal inhabitants of the north Gujrat (India). J Eco Tax Box 1998;6:193-221.
Chopra RN, Nayar SI, Chopra IC. Glossary of Indian Medicinal Plants. CSIR New Delhi India; 1956.
Nandkarni KM. Indian Materia Medica. Popular Prakashan Bombay; 1976.
Singh R, Kakkar A, Mishra VK, Bais N, Khare P. In vitro screening for anti-tuberculosis activity of Wrightia tinctoria bark and Semecarpus anacardium nuts extracts against Mycobacterium bovis by the Risazurin Microtiter assay Plate Method. JGPT 2014;6:1-3.
Madhava KC. Wrightia tinctoria Linn-chittor Medicinal plants. Himalaya Book Publication Trupathi; 2008.
Thakar PR, Tatiya AU, Surana SJ, Bhajipale NS, Deore SR. International Journal of Pharmaceutical Technology and Research 2010;2:34-34.
Madhu CD, Laksmi SD. Antiulcer activity of Wrightia tinctoria (Roxb). Pharm Sin 2011;2 Suppl 2:355-60.
Prakash A, Suneeta V. Punica granatum (Pmegranate) ride extract as a potent substitute for la ascorbic acid with respect to the antioxidant activity. Res J Pharm Biol Chem Sci 2014;5:597.
Abdullah ASH, Mirghani MES, Jamal P. Antibacterial activity of Malaysian mango kernel. Afr J Biotechnol 2011;10 Suppl 81:18739-48.
Jayamathi T, Komalavalli N, Pandiyarajan V. GC-MS analysis of leaf ethanolic extracts of Wrightia tinctoria-A high medicinal value plant. Asian J Plant Sci Res 2012;6:688-91.
Ramalakshmi S, Edaydulla N, Ramesh P, Muthuchelian K. Investigation on cytotoxic, antioxidant, antimicrobial and volatile profile of Wrightia tinctoria (Roxb.) R Br Flower used in Indian medicine. Asian Pac J Trop Dis 2012;68-75.