HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITY OF ALPINIA MALACCENSIS ROSCOE RHIZOME
Objective: The present study aims to evaluate hepatoprotective activity, antioxidant activity and total phenolic content of methanolic extract from rhizomes of A. malaccensis.
Methods: The hepatoprotective activity in mice was studied by inducing oral dose at 250 mg/kg and 500 mg/kg b. wt., antioxidant activity was evaluated by different method viz; the superoxide anion scavenging activity, hydroxyl radical scavenging activity, nitric oxide scavenging activity, DPPH radical scavenging activity and total phenolic content.
Results: The mice were significantly re established from hepatotoxicity evident by analyzing the factors such as triglycerides, ALT, AST, glucose, cholesterol, total protein, bilirubin, albumin and urea levels. Further more histopathological results revealed the significant improvement of liver. Methanolic extracts of rhizomes of A. malaccensis showed abilities to scavenge hydroxyl, superoxide free radicals, nitric oxide, DPPH with IC50 values of 50.42, 71.19, 96.38, 61.46 Âµg/ml and reducing power ability with the RP50 value of 183.54 Âµg/ml. The methanolic extract was found to have a total phenolic content of 48.18 Âµg/ml.
Conclusion: Our study reveals the therapeutic effect of A. malaccensis on treated mice as well as the strong antioxidant potential with its total phenolic content. A. malaccensis so may be used as herbal medicine to replace synthetic one.
2. Tripathi P, Dubey NK. Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruits and vegetables. Postharvest Biol Technol 2004;32:235â€“45.
3. Karthikeyan S. A statistical analysis of flowering plants of India. In: Singh NP, Singh DK, Hajra PK, Sharma BD, editors. Flora of India introductory volume (Part II). Calcutta: Botanical Survey of India; 2000. p. 201-17.
4. Thakur RS, Puri HS, Husain A. Major Medicinal Plants of India. Lucknow: CIMAP, India; 1989. p. 50-2.
5. Nuntawong N, Suksamrarn A. Chemical constituents of the rhizomes of Alpinia malaccensis. Biochem Sys Ecol 2008;36:661â€“4.
6. Abhyankar RK, Upadhyay R. Ethnomedicinal studies of tubers of Hoshangabad M. P. BEPLS 2011;1(1):57-9.
7. Bhuiyan MNI, Chowdhury JU, Begum J, Nandi NC. Essential oils analysis of the rhizomes of Alpinia conchigera Griff. and leaves of Alpinia malaccensis (Burm. f.) Roscoe from Bangladesh. African J Plant Sci 2010;4(6):197-201.
8. Habsah H, Amran M, Mackeen MM, Lajis NH, Kikuzaki H, Nakatani N, et al. Screening of Zingiberaceae extracts for antimicrobial and antioxidant activities. J Ethnopharmacol 2000;72:403-10.
9. Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC. Enzymatic determination of total serum Cholesterol. Clin Chem 1974;20(4):470-5.
10. Johnson AM, Rohifs EM, Silverman LM. Protiens. In: Burits CA and Ashwood ER, editors. The Text book of Clinical Chemistry. 3rd ed. Saunders Philadelphia; 1999. p. 477-540.
11. Moss DW, Henderson R. Clinical enzymology. In: Burtis CA, Ashwood ER, editors. Tietz textbook of clinical chemistry. 3rd ed. Philadelphia: WB Saunders Company; 1999. p. 617-721.
12. Bartholomew RJ, Delaney A. Spectrophotometric studies and analytical aplications of the protein error of some pH indicators. Proc Aust Asoc Clin Biochem 1964;1:64.
13. Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem 1969;6:24-5.
14. Thomas L. Clinical Laboratory Diagnostics. 1st ed. Frankfurt: TH-Books Verlagsgesellschaft; 1998. p. 136-46.
15. Luna LG. Mannual of histologic staining methods of Armed Force Institute of Pathology. New York: McGraw Hill Book Co; 1968.
16. Joshi S, Chanotiya CS, Agarwal G, Prakash O, Pant AK. Terpenoid compositions, and antioxidant and antimicrobial properties of the rhizome essential oils of different Hedychium species. Chem Biodivers 2008;5:299-309.
17. Oblabinri M, Odedire OO, Olaleye MT, Adekunle AS, Ehigie LO, Olabinri PF. In vitro evaluation of hydroxyl and nitric oxide radical scavenging activities of Artemether. Res J Biol Sci 2010;5:102-5.
18. Naskar BS, Islam, A. Mazumder UK, Saha P, Haldar PK, Gupta M. In vitro and in vivo antioxidant potential of hydromethanolic extracts of Phoenix dactylifera fruits. J Sci Res 2010;2:144-57.
19. Nishimiki M, Rao NA, Yagi K. The occurance of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem Biophys Res Commun 1972;46:849-53.
20. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdicâ€“phosphotungstic acid reagents. Am J Enol Viticult 1965;16:144â€“58.
21. Opoku AR, Ndlovu IM, Terblanche SE, Hutchings AH. In vivo hepatoprotective effects of Rhoicissus tridentate subsp. Cuneifolia, a traditional Zulumedicinal plant against CCl4-induced acute liver injury in rats. S Afr J Bot 2007;73(3):372â€“7.
22. Johnston, DE, Kroening D. Mechanism of early carbon trtrachloride toxicity in cultured rat hepatocytes. Pharmacol Toxicol 1998;83:231-9.
23. Gonzalez R, Anchota O, Pascual C, Pallon R, Frutos N, Milian V. Hepatoprotective properties of lobenzarit in the rat. Biotechnol Appl 1991;8:140-7.
24. Mitra SK, Venkataranganna MV, Sundaram R, Gopumadhavan S. Protective effects of HD-03, A herbal formulation, against various hepatotoxic agents in rats. J Ethnopharmacol 1998;63:181-6.
25. Afolayan AJ, Yakubu MT. Effect of bulbine natalensis baker stem extract on the functional indices and histology of the liver and kidney of male wistar rats. J Med Food 2009;12(4):814â€“20.
26. Sasidharan S, Aravindra S, Latha LY, Vijenthi R, Saravanan D, Amutha S. In vitro antioxidant activity and hepatoprotective effects of lentinula edodes against paracetamol induced hepatotoxicity. Mol 2010;15(6):4478â€“89.
27. Asha VV. Preliminary studies on the hepatoprotective activity of Mamordica subangulata and Nargamia alata. Indian J Pharm 2001;33:276-9.
28. Thabrew MI, Joice PD, Rajasissa W. Comparative study of the efficacy of Pavetta indica and Osbeckia octandra in treatment of liver dysfunction. Plant Med 1987;53(3):239-41.
29. Kaplan MM. Laboratory tests. In: Schiff L, Schiff ER editors. Diseases of the liver. 7th ed. Philadelphia: Lippincott; 1993. p. 108-44.
30. Kamath PS. Clinical approach to the patient with abnormal liver function test results. Mayo Clin Proc 1996;71:1089-94.
31. Goddard CJ, Warnes TW. Raised liver enzymes in asymptomatic patient investigation and outcome. Dig Dis 1992;10:218-26.
32. Quinn PG, Johnston DE. Detection of chronic liver disease: costs and benefits. Gastroenterol 1997;5(1):58-77.
33. Awang D. Milk Thistle. Can Pharm J 1993;126:403-4.
34. Matthaus B. Antioxidant activity of extracts obtained from residues of different oilseeds. J Agricult Food Chem 2002;50:3444-52.
35. Sreedhar V, Nath LKR, Gopal NM, Nath MS. In-vitro antioxidant activity and free radical scavenging potential of roots of Vitex trifoliate. Res J Pharm Biol Chem Sci 2010;1:1036-44.
36. Siddhuraju P, Mohan PS, Beaker K. Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.). A preliminary assessment of crude extracts from stem, bark, leaves and fruit pulp. Food chem 2002;79:61-7.
37. Skerget M, Kotnik P, Hadolin M, Hras AR, Simonic M, Knez Z. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem 2005;89:191-8.