SECONDARY METABOLITES FROM RICE CULTURE OF ASPERGILLUS SP. ISOLATED FROM MELALEUCA SUBULATA (CHEEL) CRAVEN LEAVES AND THEIR ANTICANCER ACTIVITY

  • HAITHAM ALI IBRAHIM Pharmacognosy Department, Faculty of Pharmacy, Helwan University
  • REHAM RAGAEI IBRAHIM Pharmacognosy Department, Faculty of Pharmacy, Helwan University
  • REEM ALAA KAMEL Mansheyat El-Bakry, General Hospital, Heliopolis
  • SHAHENDA METWALLY EL-MESSERY Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Mansoura University
  • FATMA ABDELKADER MOHARRAM Pharmacognosy Department, Faculty of Pharmacy, Helwan University

Abstract

Objective: Aspergillus fungus is a rich source of natural products with broad biological activities. This study was conducted to identify secondary metabolites from the rice culture of Aspergillus species isolated from Melaleuca subulata leaves and evaluated their anticancer activity.


Methods: Ethyl acetate extract was fractionated on silica gel and Sephadex columns. Structures of the compounds were established using physical and chemical methods. Cytotoxic activities of the extract and pure compounds against two human cancer cell lines (Mcf-7and Hep G2) were evaluated using microculture tetrazolium assay as well as the mode of the cytotoxicity was evaluated. Molecular docking studies have been performed using the Hsp 90 enzyme as an anticancer target.


Results: Methyl linoleate (1), arugosin C (2), ergosterol (3), sterigmatocystin (4), diorcinol (5), alternariol-5-O-methyl ether (6), averufin (7), averufanin (8), and alternariol (9) were identified from ethyl acetate extract. All tested compounds exhibit week activity against MCF-7 and Hep G2 cell lines but a mixture of compounds 7 and 8 is considered to be more active towards both MCF-7 and Hep G 2 in comparison to other compounds. Compound 4 exhibits moderate activity against Hep G2 only as well as the ethyl acetate extract exerts moderate activity against MCF-7 cell line Moreover, compound 4 and a mixture of 7 and 8 caused a decrease in the number of Hep G2 cancer cells due to apoptotic and necrotic processes. Most active anticancer candidates 7 and 8 showed binding to the active site similar to geldanamycin reference ligand.


Conclusion: Secondary metabolites identified from Aspergillus sp. and their anticancer activity were evaluated. Molecular docking suggested active candidates as Hsp 90 inhibitors.

Keywords: Anthraquinones, Apoptotic, Aspergillus sp, Melaleuca subulata, Necrotic, Xanthone

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IBRAHIM, H. A., R. R. IBRAHIM, R. A. KAMEL, S. M. EL-MESSERY, and F. A. MOHARRAM. “SECONDARY METABOLITES FROM RICE CULTURE OF ASPERGILLUS SP. ISOLATED FROM MELALEUCA SUBULATA (CHEEL) CRAVEN LEAVES AND THEIR ANTICANCER ACTIVITY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 10, Sept. 2020, pp. 52-61, doi:10.22159/ijpps.2020v12i10.38773.
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