ATTENUATION OF ANTINOCICEPTIVE EFFECT OF MORPHINE IN DIABETIC MICE: NITRIC OXIDE OR INTERLEUKIN-2
Keywords:
Analgesic, Cyclosporin, Diabetes, HyperalgesiaAbstract
Objective: The present study was designed to explore the mechanistic role of interleukin-2 in diabetes-induced decrease in the antinociceptive effect of morphine in mice. Role of interleukin-2 was investigated by employing cyclosporin, a interleukin-2 synthesis inhibitor.
Methods: Diabetes was induced in mice by single intra peritoneal injection of Streptozotocin (200 mg/kg, i. p.). Nociceptive threshold in diabetic mice was measured by Rodent tail-flick test. Nitrite levels in the urine of mice were estimated by employing Greiss reagent.
Results: A significant decrease in antinociceptive effect of morphine was observed in mice. Administration of cyclosporin (20 mg/kg, s. c., b. d.) in diabetic mice significantly increased antinociceptive effect of morphine in diabetic mice. However, administration of cyclosporin (20 mg/kg, s. c., b. d.) failed to significantly change the increased nitrite levels in diabetic mice.
Conclusion: The present study indicates that interleukin-2 may be responsible for decrease in antinociceptive effect of cyclosporine. The study also indicates that the increase in levels of interleukin-2 is independent of an increase in nitrite levels. It may, therefore, be concluded that nitric oxide has no role in nociceptive changes made by interekin-2 in diabetic mice.
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