NOVEL FINDINGS OF PHOENIX DACTYLIFERA, DATES FRUIT EARLY-STAGE HABABUK FOR PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND CYTOTOXIC POTENTIAL
Aim: The present study aims to investigate the phytochemical constituents, antioxidant, and cytotoxic activity of the total ethanolic extract of the hababuk stage of date’s fruits (PDHE), Phoenix dactylifera.
Methods: The date fruits early stage hababuk was collected authenticated, shade dried and powdered in an electrical grinder. The powdered plant part was extracted using ethanol. Qualitative phytochemical screening, were carried out by established procedures. The in vitro antioxidant potentials were performed by diphenyl-2-picrylhydrazyl, azino-bis (3-ethylbenzothiazoline-6-sulphonic acid and nitric oxide scavenging assays. The acute cytotoxicity study carried out by brine shrimp lethality assay and cell lines study using three different cell lines Lung Cancer (A549), Human cervical carcinoma (HeLa) and Breast Cancer (MCF)-7 cells by MTT method.
Results: The phytochemical screening using standard procedure and showed the presence of important constituents like alkaloids, glycosides, phenolic, flavonoids, carbohydrate, proteins, amino acid, terpenoids, steroids, and saponins. The antioxidant studies of the PDHE compared with standard showed very excellent results IC50 values for DPPH activity and nitric oxide. A good result was also shown by ABTS when compared with that of standard. The brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. The extract showed excellent LC50 value which correlates with cytotoxicity studies previously established. The MTT study results using the three different cell lines compared with the standard doxorubicin, promising results obtained for HeLa cell lines when compared with other cell lines.
Conclusion: The observed significant free radical scavenging activity along with in vitro cytotoxic potentials results suggests the hababuk stage of the plant part may be a potential source for the identification of various pharmacologically active phytochemicals capable of controlling oxidative stress and anticancer potentials.
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