NOVEL FINDINGS OF PHOENIX DACTYLIFERA, DATES FRUIT EARLY-STAGE HABABUK FOR PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND CYTOTOXIC POTENTIALS
Keywords:Date fruit, Ethanolic extract, Antioxidant, Hababuk, Brine shrimp, Phytochemical screening
Objective: The present study aims to investigate the phytochemical constituents, antioxidant, and cytotoxic activity of the total ethanolic extract of the hababuk stage of date’s fruits Phoenix dactylifera (PDHE).
Methods: Extracts PDHE obtained with shade dried and a powdered sample of hababuk stage of date fruits Phoenix dactylifera and subjected to phytochemical screening, in vitro antioxidant activity study by DPPH assay and ABTS assay, in vitro cytotoxic studies carried out for three different cell lines Lung Cancer (A549), Human cervical carcinoma (HeLa) and Breast Cancer (MCF)-7 cells by MTT method and brine shrimp lethality assay using standard procedure.
Results: The phytochemical study revealed the presence of important constituents like alkaloids, glycosides, phenolic, flavonoids, carbohydrate, proteins, amino acids, terpenoids, steroids, and saponins. The antioxidant studies of the PDHE compared with standard showed very excellent results IC50 values for DPPH activity and nitric oxide. A good result was also shown by ABTS when compared with that of standard. The brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. The extract showed excellent LC50 value, which correlates with cytotoxicity studies previously established. The MTT study results using the three different cell lines compared with the standard doxorubicin, promising results obtained for HeLa cell lines when compared with other cell lines.
Conclusion: Results obtained from the present work indicated that the hebabuk stage of date fruit is a promoising antioxidant and anticancer agent. Further study should be carried out to isolate the bioactive compound responsible for the activity.
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