Optimized and Validated Spectrophotometric Method for the Determination of Ampicillin in Pharmaceutical Formulations

Determination of Ampicillin in Pharmaceutical Formulations


A simple and accurate spectrophotometric method have been proposed for the determination of ampicillin in pharmaceutical formulations. The method is based on the reaction of the carboxylic acid group of ampicillin with a mixture of potassium iodate (KIO3) and iodide (KI) to form a yellow-colored product in an aqueous medium at room temperature. The reaction is followed spectrophotometrically by measuring the absorbance at 352 nm (λmax). Beer’s law is obeyed in the concentration range of 0.25–2.5 µg/ml. The method is validated according to international conference on harmonization (ICH) guidelines in terms of specificity, linearity, linearity range, accuracy, precision, the limit of detection, the limit of quantitation, robustness, and ruggedness. The percent recoveries, relative standard deviation (RSD), and standard analytical error (SAE) for the method were also determined. The application of the proposed method to commercial dosage forms is presented.


Keywords: Ampicillin, Pharmaceutical formulations, validation, spectrophotometer


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HAQUE, S. M. “Optimized and Validated Spectrophotometric Method for the Determination of Ampicillin in Pharmaceutical Formulations: Determination of Ampicillin in Pharmaceutical Formulations”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 13, no. 2, Jan. 2021, https://innovareacademics.in/journals/index.php/ijpps/article/view/40025.
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