The Extraction, Isolation of Active Principles, Anti-Bacterial & Wound Healing Activity of the Marine Algal Species Oedogonium Globosum & Oedogonium Intermedium

Isolation of Phyto-constituents of Marine Algal species and their Pharmacological Activities

  • Dr. M. Karpakavalli Karpagam College of Pharmacy
  • Prakash Govindaraj
  • Sivasubramaniam Piramanayagam
  • Ranjithkumar Dhanaraj
  • Mohan Sellappan

Abstract

Objectives: Algae are the undisputed treasures of the sea and are valuable raw material, providing unlimited opportunities for new drug discoveries. Marine algal products are in demand in the international market in the form of standardized algal extracts or semi-finished products.


Methods: Aqueous and methanolic extracts of Oedogonium globosum and Oedogonium intermedium species were obtained maceration and hot percolation. The active principles from O. intermedium were isolated, purified by column chromatography, and characterized by spectral studies IR, λmax, 1HNMR & MS. The extracts of Oedogonium species were screened for their anti-microbial effects, acute dermal irritation and wound-healing activity studies.


Results: Comparing to Oedogonium intermedium (45 %, 90 %, 87 %), very low extractive yields were obtained for Oedogonium globosum (10.80 %, 37 %, 28 %). On phytochemical screening, Terpenoids, Flavanoids and, Glycans were found to be present in a significant amount and upon their isolation, it was found that a collection of fractions from cold extract with Rf value in the range 0.32-0.34 as Glycans and those from the hot extract with 0.40-0.72 as Flavanoids and those from methanolic extracts with 0.23, 0.44 and 0.71 as for Terpenoids. Anti-bacterial study revealed out the fact of Oedogonium species could give higher inhibition to gram-positive than for gram-negative bacteria at (10 μg/10μl/disc) concentration. No symptoms of systemic toxicity and mortality were observed. Silver sulfadiazine, more potent in wound closure, the effect of methanolic extracts of O. intermedium (87 %) was almost at par to the standard (95 %) in action and significantly greater than O. globosum (72 %, P<0.05).


Conclusion: Admittedly, Oedogonium type algal species can be known as medicinal algae with a plethora of a wide range of pharmacological activities. Thus, this research work may be considered further for extensive innovative discoveries of new lead molecules and any other pharmacological activities, in future.


 

Keywords: Oedogonium Globosum, Oedogonium Intermedium, Extraction, Phytochemical analysis, Isolation, Dermal irritation test, Anti-microbial and Wound healing Activities

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References

Rajasulochana P, Preethy V. Glimpses on cosmetic application using marine red algae. Res Int J Pharmaceut Tech 2015; 7: 9235-9242.
2. Aurelio F, Tonetto SM, Kassandra, Oliveira M, Tatiana, Domingues. Analysis of biochemical composition of three stream macroalgae species. Int J Appl Sci Tech 2014; 4: 111-123.
3. Mariana NS, Neela VK, Nik Kani MA, Norfarrah S, Zamberi S. In vivo evaluation on Malaysian coastel isolates of Gracilaria changii and Stichopus badionotus through heat-burn methicillin resistant Staphylococcus aureus (MRSA) infection animal model. Res African J Microbiol 2011; 5: 1379-1382.
4. Adithya T, Bitu M, Eleanor. The Role of algae in pharmaceutical development. Res J Pharmaceut Nanotech 2016; 4: 82-89.
5. Rajasulochana P, Krishnamoorthy, Dhamotharan. Isolation of identification of bromophenol compound and antibacterial activity of Kappaphy cussp. Res Int J Pharm Biol Sci 2012; 3: 173-186.
6. Shyamala V, Ebciba, Santhiya N, Thangaraju. Phytochemical screening and In vitro antibacterial, antioxidant and anticancer activity of Amphiroa fragilissima (Linneaus) V, Lanmoroux. Res Int J Innov Res Sci 2014; 3: 12933-12948.
7. Harsha KD, Joshi D, Preeti P, Manish KP, Amit CK. Algae as future drug. Res Asian J Pharm Clin Res 2012; 5: 1-4.
8. Devaraj ID, Paramasivam G, Rajalakshmi J, Solomon J. Antioxidant activities of marine algae Valoniopsis pachynema and Sargassum swartzii from the south east coast of India. Int J Fisher Aqua Stud 2015; 3: 426-430.
9. Seenivasan R, Archana G, Geetha S, Indu H. The antibacterial and anti oxidant activity of some marine algae from south east coast of India. Res American-Eurasian J Agri Environ Sci 2010; 9: 480-489.
10. Sujatha L, Subba RG, Lalitha GT. Antibacterial activity of green seaweeds on oral bacteria. Nat Prod 2012; 3: 328-333.
11. Rabia A, Salah A, Fauzi W, Abduel R, Fatiem G. Screening of antibacterial activity in marine green, red and brown macro algae from the western coast of Libya. Nat Sci 2013; 5: 7-14.
12. Stephen S, Timothy J, Entwisle. New taxa and combinations for Oedogonium and bulbochaete odeogoniales,chlorophyceae) in Australia, Telopea 2006; 11: 171-194.
13. Santhanam S, Aseer M, Sugathan S, Joseph, George S. Antibacterial activity of seaweeds extracts against multi-resistant pathogens. Ann Microbiol 2008; 58: 535-541.
14. David L, Ballantine G, Pablo H, William, Gerwick M, Sylvia E, Velez. Antibiotic activity
of lipid-soluble extracts from Caribbean marine algae. Hydrobiol 1987; 151: 463-469.
15. Fedekar F, Madkour MH, Mohsen K, Waleed S, Walied. Wound healing activity of brown algae plus polyherbal extract in normal and alloxan-induced diabetic rats. Res J Adv Vet 2013; 3:102-108.
16. Kumara SHM, Krishna V, Shankarmurthy K, Abdul RB, Mankani KL, Mahadevan KM. Wound healing activity of embelin isolated from the ethanol extract of leaves of Embelia ribes Burm. J Ethnopharmacol 2007; 109: 529-34.
17. Izabela M. Algal extracts: Technology and Advances. English Life Sci. 2014; 14: 581-591.
18. Sarah S, Abdu-llah Al-S, Nevein A, Hend A, Wazanani AA, Ibrahim AA. Antibacterial substances from marine algae isolated from Jeddah coast of red sea, Saudi Arabia. Saudi J Biol Sci 2014; 24:57-64.
19. Adithya T, Bitu, E, Mercy. The Role of algae in pharmaceutical development. Res J Pharmaceut Nanotech 2016; 4: 82-89.
20. Dalia F, Muhammad El-Saadani. Phytochemical constituents and bio-screening activities of green algae (Ulva lactuca). Res Int J Agri Policy 2014; 2: 373-378.
21. Victoria N, Emmanuel E, Ilodigwe L, Daniel O, Earnest, Erhirhie E, Goodies Moke, Mand AA. Wound healing Activity of the Aqueous Leaf Extract and Fractions of Ficus exasperata (Moraceae) and its Safety Evaluation on Albino Rats. J Trad Complemen Med 2014; 4: 246-252.
22. Lucy M, Premalatapati RP, Subrat KB. Therapeutic health booster seaweeds against several maladies. Indian J Geo-Marine Sci 2013; 42: 538-546.
23. Santhanam S, Aseer M, Sugathan S, Joseph S, George S. Antibacterial activity of seaweeds extracts against multi-resistant pathogens. Ann Microbiol 2008; 58: 535-541.
24. Shimaa M, Shafay EI, Samh Ali M, Mostafa, EI-Sheekh. Antibacterial activity of some sea weeds species from red sea, against multidrug resistant bacteria. Egyptian J Aqua Res 2016; 42: 65-74.
25. Panigrahi BB, Panda PK, Patro VJ. Wound healing activity of Spirulina extracts. Res Int J Pharmaceut Sci 2011; 6: 132-135.
26. Prashanthkumar P, Angadi BS, Vidyasagar GM. Antimicrobial and anti-inflammatory activity of blue green algae. Res Indian J Pharmaceut Sci 2006; 68: 647-648.
27. Turkiyede Y, Bazi C, Otlarinin Y, Iyilestirici E. Wound healing potential of selected liverworts growing in Turkey. Res Turkish J Pharmaceut Sci 2016; 13: 285-291.
28. Lucy M, Premalatapati R, Panigrahy, Subrat KB. Therapeutic health booster seaweeds against several maladies. Indian J Geo-Marine Sci 2013;42: 538-546.
29. Fedekar F, Madkour, Mohsen MH, Waleed K, Walied S. Wound healing activity of brown algae plus polyherbal extract in normal and alloxan-induced diabetic rats. Res J Adv Vet, 2013;3: 102-108.
30. Sasidharan Y, Chen D, Saravanan KM, Sundram L, Yoga L. Extraction, Isolation and Characterization of Bioactive Compounds from Plants’ Extracts. African J Trad Comple Alt Med 2011; 8: 1-10.
31. Lakshmi S, Padmaja G, Remani P. Anti-tumour Effects of Isocurcumenol Isolated from Curcuma zedoaria Rhizomes on Human and Murine Cancer Cells. Int J Med Chem, 2011; 13.
32. Ganesan M, KannanL. Iron and Manganese Concentrations in Seawater, Sediment and Marine Algae of Tuticorin Coast, Southeast Coast of India. Indian J Marine Sci 1995; 24: 236-237.
33. Indira P, Biswajit R. Commercial and industrial applications of micro algae. J Algal Biomass Utln 2012; 3: 89-100.
34. Karodi R, Jadhav M, Rub R, Bafna A. Evaluation of the wound healing activity of a crude extract of Rubia cordifolia L. (Indian madder) in mice. Int J Appl Res Nat Prod, 2009;2: 12- 18.
35. Ashoka BVL, Goravanakolla A, Murali A, Madhava V, Yoganarasimhan SN. Wound healing activity of the leaves of Wattakaka volubilis (L.f.) Stapf (Asclepiadaceae). Int J of Appl Res Nat Prod, 2012; 5: 23-29.
36. Seung HH, Jai MJ, Soon SL. Isolation of fucosterol from Pelvetiasi liquosa by High-Speed counter current chromatography. Fish Aqua Sci 2012; 15:191-195.
37. Xiao-HX, Zhi-QY, Gong-KL. Preparation of phytosterols and phytol from edible marine algae by Microwave-assisted extraction and high-speed counter current chromatography. Res Sepn Purif Tech 2012; 104: 284-289.
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Dr. M. Karpakavalli, Prakash Govindaraj, Sivasubramaniam Piramanayagam, Ranjithkumar Dhanaraj, and Mohan Sellappan. “The Extraction, Isolation of Active Principles, Anti-Bacterial & Wound Healing Activity of the Marine Algal Species Oedogonium Globosum & Oedogonium Intermedium: Isolation of Phyto-Constituents of Marine Algal Species and Their Pharmacological Activities”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 13, no. 5, Apr. 2021, doi:10.22159/ijpps.2021v13i5.40026.
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