Use of CBNAAT in rapid detection of mycobacterium tuberculosis

  • Rashmi M Karigoudar Shri B M Patil Medical college and research centre vijayapur


Introduction: Tuberculosis (TB) is an airborne infection caused by Mycobacterium tuberculosis. India continued at the top record with tuberculosis (TB) and with increased number of drug-resistant TB cases according to 2019 World Health Organization report. With a timely diagnosis and treatment, most people with TB can be cured and further spread of infection can be reduced.  Prompt and accurate diagnosis of TB is necessary for early and well-organized treatment. Cartridge-based nucleic acid amplification test (CBNAAT) provides a valuable tool in the early detection of TB. Aims and objectives: Use of CBNAAT testing for the detection of tuberculosis (TB) and to compare CBNAAT with ZN staining. Material and Methods: This prospective observational study was carried out in the Department of Microbiology, BLDEU's Shri B. M. Patil Medical College, Hospital & RC & Dr. Karigoudar Diagnostic Laboratory, Vijayapur. A total of 129 samples from patients of any age, with presumptive diagnosis of TB on the basis of history, clinical presentation and radiological finding were included in the study. All samples were subjected to ZN staining and Cartridge-based nucleic acid amplification test and data were analysed. Results: The present study showed ZN smear positivity of 7.75% and CBNAAT positivity of 19.38%.   CBNAAT sensitivity and specificity was 90% and 86.55% respectively when compared with ZN staining with a significant P value of<0.001. Conclusion: Cbnaat not only helps in timely diagnosis of TB with high sensitivity and specificity but also detects rifampicin resistance within 2-3 hours. Rifampicin resistance detection is of great concern which otherwise leads to treatment failure and on time spread of multidrug resistance TB leading to increased morbidity and  mortality.
Keywords: Tuberculosis, CBNAAT, Rifampicin resistance


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1) Tuberculosis. WHO Global Tuberculosis Report 2019.
2). Arora D, Jindal N, Bansal R, Arora S. Rapid Detection of Mycobacterium tuberculosis in Sputum Samples by Cepheid Xpert Assay: A Clinical Study. J Clin Diagn Res. 2015 May;9(5):DC03-5
3) Sachdeva K, Shrivastava T. CBNAAT: A Boon for Early Diagnosis of Tuberculosis-Head and Neck. Indian J Otolaryngol Head Neck Surg. 2018 Dec;70(4):572-577.
4) Scott LE, Beylis N, Nicol M, Nkuna G, Molapo S, Berrie L, Duse A, Stevens WS. Diagnostic accuracy of Xpert MTB/RIF for extrapulmonary tuberculosis specimens: establishing a laboratory testing algorithm for South Africa. J Clin Microbiol. 2014 Jun;52(6):1818-23
5) Munir MK, Rehman S, Aasim M, Iqbal R, Saeed S. Comparison of Ziehl Neelsen Microscopy with GeneXpert for Detection of MycobacteriumTuberculosis. IOSR Journal of Dental and Medical Sciences. 2015; 14. 56-60
6) Mukherjee S, Biswas D, Begum S, et al. Evaluation of cartridge based nucleic acid amplification test in diagnosis of pulmonary tuberculosis. J. Evolution Med. Dent. Sci. 2017;6(74):5281-5286.
7) Mostaza J L, Garcia N, Fernandez S, Bahamonde A, Fuentes M I, Palomo M J. Analysis and predictor of delay in suspicion and treatment among hospitalized patients with pulmonary tuberculosis. An Med Interna. 2007; 24(10): 478-83.
8) Ramirez HL, García-Clemente MM, Alvarez-Álvarez C, et al. Impact of the Xpert MTB/RIF molecular test on the late diagnosis of pulmonary tuberculosis. The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease. 2014 Apr;18(4):435-437
9) Bajrami R, Mulliqi G, Kurti A, Lila G, Raka L. Comparison of GeneXpert MTB/RIF and conventional methods for the diagnosis of tuberculosis in Kosovo. J Infect Dev Ctries. 2016;10(4):418–422
10) Kandi S, Reddy V, Nagaraja SB.Diagnosis of pulmonary and extra pulmonary tuberculosis: How best is CBNAAT when compared to conventional methods of TB detection? Pulm Res Respir Med Open J. 2017; 4(2): 38-41.
11) Panayotis I, Papaventsis D, Karabela S, et al. Cepheid GeneXpert MTB/RIF assay for Mycobacterium tuberculosis detection and rifampin resistance identification in patients with substantial clinical indications of tuberculosis and smear-negative microscopy results. J Clin Microbiol. 2011; 49(8): 3068-3070.
12) Armand S, Vanhuls P, Delcroix G, Courcol R, Lemaître N. Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens. J Clin Microbiol. 2011;49(5):1772–1776.
13) Steingart KR, Schiller I, Horne DJ, Pai M, Boehme CC, Dendukuri N. Xpert® MTB/RIF assay for pulmonary tuberculosis and rifampicin resistance in adults. Cochrane Database Syst Rev. 2014;2014(1):CD009593.
14) Theron G, Pooran A, Peter J. Do adjunct tuberculosis tests, when combined with Xpert MTB/RIF, improve accuracy and the cost of diagnosis in a resource poor settings? EurRespir J. 2012; 40:161-68
15). Boyles TH, Hughes J, Cox V, Burton R, Meintjes G, Mendelson M. False-positive Xpert(®) MTB/RIF assays in previously treated patients: need for caution in interpreting results. Int J Tuberc Lung Dis. 2014;18(7):876-78.
16) Basavaraj VP, Rajani R. CBNAAT: A Novel Tool for Rapid Detection of MTB and Rifampicin Resistance. Int. J. Curr. Microbiol. App. Sci. 2016;5(12):383-8.
17) Road map for rolling out Xpert MTB/RIF for rapid diagnosis of TB and MDR-TB. WHO 2010.
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Karigoudar, R. M. “The USE OF CARTRIDGE BASED NUCLEIC ACID AMPLIFICATION TEST FOR RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS : Use of CBNAAT in Rapid Detection of Mycobacterium Tuberculosis ”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 13, no. 2, Jan. 2021,
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