The USE OF CARTRIDGE BASED NUCLEIC ACID AMPLIFICATION TEST FOR RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS

Use of CBNAAT in rapid detection of mycobacterium tuberculosis

  • Rashmi M Karigoudar Shri B M Patil Medical college and research centre vijayapur

Abstract

Abstract:
Introduction: Tuberculosis (TB) is an airborne infection caused by Mycobacterium tuberculosis. India continued at the top record with tuberculosis (TB) and with increased number of drug-resistant TB cases according to 2019 World Health Organization report. With a timely diagnosis and treatment, most people with TB can be cured and further spread of infection can be reduced.  Prompt and accurate diagnosis of TB is necessary for early and well-organized treatment. Cartridge-based nucleic acid amplification test (CBNAAT) provides a valuable tool in the early detection of TB. Aims and objectives: Use of CBNAAT testing for the detection of tuberculosis (TB) and to compare CBNAAT with ZN staining. Material and Methods: This prospective observational study was carried out in the Department of Microbiology, BLDEU's Shri B. M. Patil Medical College, Hospital & RC & Dr. Karigoudar Diagnostic Laboratory, Vijayapur. A total of 129 samples from patients of any age, with presumptive diagnosis of TB on the basis of history, clinical presentation and radiological finding were included in the study. All samples were subjected to ZN staining and Cartridge-based nucleic acid amplification test and data were analysed. Results: The present study showed ZN smear positivity of 7.75% and CBNAAT positivity of 19.38%.   CBNAAT sensitivity and specificity was 90% and 86.55% respectively when compared with ZN staining with a significant P value of<0.001. Conclusion: Cbnaat not only helps in timely diagnosis of TB with high sensitivity and specificity but also detects rifampicin resistance within 2-3 hours. Rifampicin resistance detection is of great concern which otherwise leads to treatment failure and on time spread of multidrug resistance TB leading to increased morbidity and  mortality.
Keywords: Tuberculosis, CBNAAT, Rifampicin resistance

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References

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Karigoudar, R. M. “The USE OF CARTRIDGE BASED NUCLEIC ACID AMPLIFICATION TEST FOR RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS : Use of CBNAAT in Rapid Detection of Mycobacterium Tuberculosis ”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 13, no. 2, Jan. 2021, https://innovareacademics.in/journals/index.php/ijpps/article/view/40055.
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