H. SABDARIFFA CALYCES ASH: ANTIANXIETY AND ANTIDEPRESSANT ACTIVITY EVALUATION AND PREPARATION OF ITS TABLETS

  • GULSHEEN University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India
  • ASHWANI KUMAR University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India
  • ANUPAM SHARMA University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India

Abstract

Objective: Ash and its preparations have been used in Ayurveda for the treatment of various ailments since 7th century AD. Numerous studies suggest that the elements present in ash have significant role in affective disorders. Thus, the present study aimed at evaluating the antianxiety and antidepressant activity of total ash of H. sabdariffa calyces, and also of the tablets of total ash.


Methods: Powdered calyces were taken in tared silica crucible, and were incinerated at a temperature not exceeding 450 °C. The resultant ash was cooled and weighed. This was evaluated for antianxiety and antidepressant activity using an elevated plus maze and Porsolt’s swim test, respectively. Further, the ash samples were analysed through ICP-MS to know their composition. The ash was formulated into tablets using the wet granulation technique, using only organic excipients.


Results: Results indicated that the total ash of H. sabdariffa calyces exhibited significant (p<0.001) antianxiety and antidepressant activity at 50 and 100 mg/kg, po, respectively. The activities were comparable to the standard drugs. ICP-MS analysis showed the presence of magnesium, phosphorous, potassium and calcium as major elements. Tablets of H. sabdariffa total ash were as effective (p<0.001) as the total ash.


Conclusion: Magnesium, phosphorous, potassium and calcium have been reported to play a significant role in affective disorders, explaining, thereby, as to why ash of H. sabdariffa calyces exhibited anti-anxiety and antidepressant activity.

Keywords: Total ash, Antianxiety, Antidepressant, Elevated plus maze, Porsolt’s despair test, Tablets, ICP-MS, Elements

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References

1. Depression and other common mental disorders: global health estimates, World Health Organization; 2020.
2. Nuss P. Anxiety disorders and GABA neurotransmission: a disturbance of modulation. Neuropsychiatr Dis Treat 2015;11:165-75.
3. Fried EI, Epskamp S, Nesse RM, Tuerlinckx F, Borsboom D. What are 'good' depression symptoms? Comparing the centrality of DSM and non-DSM symptoms of depression in a network analysis. J Affect Disord 2016;189:314-20.
4. Zhiguo WU, Yiru FANG. Comorbidity of depressive and anxiety disorders: challenges in diagnosis and assessment. Shanghai Arch Psychiatry 2014;26:227-31.
5. Guzman Gutierrez SL, Chilpa RR, Jaime HB. Medicinal plants for the treatment of nervios, anxiety, and depression in mexican traditional medicine. Rev Bras Farmacogn 2014;24:591-608.
6. Patricia M, Barnes MA, Barbara Bloom MPA. National health statistics reports; no 12. National center for Health Statistics. USA, Hyattsville; 2008.
7. Chaikelis AS. The thiamine content of herbs and medicinal plants. J Am Pharm Assoc 1946;35:343-6.
8. Pal S. The ayurvedic bhasma: the ancient science of nanomedicine. Recent Patents Nanomed 1946;5:12-8.
9. Mlyniec K, Davies CL, de Aguero SIG, Pytka K, Budziszewska B, Nowak G. Essential elements in depression and anxiety. Part 1. Pharmacological Reports 2014;66:534-44.
10. Mlyniec K, Gawel M, Doboszewska U, Starowicz G, Pytka K, Davies CL, et al. Essential elements in depression and anxiety. Pharmacol Reports 2015;67:187-94.
11. Sastri BN. The wealth of India, a dictionary of Indian raw material and Industrial products. Vol. 3. CSIR, New Delhi; 1952.
12. Amos S, Binda L, Chindo BA, Tseja A, Odutola AA, Wambebe C, et al. Neuropharmacological effects of Hibiscus sabdariffa aqueous extract. Pharm Biol 2003;41:325-9.
13. Panesar G, Kumar A, Sharma A. In vivo antianxiety and antidepressant activity of calyx extracts. J Pharm Res 2017;11:962-6.
14. Ismail Z, Malaysia KK, Ismail N, Lassa J. Malaysian herbal monograph; 1999.
15. The United States Pharmacopoeia. Vol. 2. United Book Press, Inc; 2014.
16. OECD Guidelines for testing of chemicals: Acute oral toxicity-Acute toxic class method. Test no. 423, OECD, Paris; 2001.
17. Pellow S, Chopin P, File SE, Briley M. Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 1985;14:149-67.
18. Vogel HG, Vogel WH. Drug discovery and evaluation: pharmacological assays. 1st ed. Springer Berlin Heidelberg; 2013.
19. Porsolt RD, Anton G, Blavet N, Jalfre M. Behavioural despair in rats: a new model sensitive to antidepressant treatments. Eur J Pharmacol 1978;47:379-91.
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GULSHEEN, A. KUMAR, and A. SHARMA. “H. SABDARIFFA CALYCES ASH: ANTIANXIETY AND ANTIDEPRESSANT ACTIVITY EVALUATION AND PREPARATION OF ITS TABLETS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 13, no. 4, Apr. 2021, pp. 33-38, doi:10.22159/ijpps.2021v13i4.40264.
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