• Aakruti Kaikini Bharati Vidyapeeth's College of Pharmacy
  • Swati Dhande Bharati Vidyapeeth's College of Pharmacy
  • Vilasrao Kadam Bharati Vidyapeeth's College of Pharmacy


Objective: The aim of present study was to investigate a dose-dependent antihyperlipidemic effect of methanolic extract of Bambusa bambos Druce. (Family: Graminae) (MEBB) in Poloxamer-407 induced hyperlipidemia in rats at three different dose levels i. e. 100 mg/kg, 200 mg/kg and 300 mg/kg.

Methods: Animals were divided randomly into 6 groups: Group I (Vehicle Control), Group II (Disease Control), Group III (100 mg/kg MEBB), Group IV (200 mg/kg MEBB), Group V (300 mg/kg MEBB) and Group VI (Standard Control).

Results: Groups treated with the MEBB showed significant (p<0.01) decrease in serum total cholesterol, triglycerides, LDL-cholesterol VLDL-cholesterol, hepatic cholesterol and hepatic triglycerides with the concomitant increase in serum HDL-cholesterol levels. The extract also showed significant (p<0.01) antioxidant activity in liver tissue homogenate. Atherogenic indices also showed significant (p<0.05, p<0.01) decrease in treatment groups as compared to disease control group. The most effective dose was found to be 300mg/kg MEBB.

Conclusion: These results suggest that methanolic extract of Bambusa bambos leaves possess significant antihyperlipidemic activity.


Keywords: Antihyperlipidemic activity, Poloxamer-407, Bambusa bambos, Atherosclerosis


Download data is not yet available.


1. Third report of National Cholesterol Education Program (NCEP) Expert Panel on detection, evaluation and treatment of high blood cholesterol in adults (Adults treatment panel III) Final report. Circ 2002;106:3143-421.
2. Bertolotti M, Mawantonio M, Gabbi C, Anzivino C, Carulli N. Review article: Hyperlipidemia and cardiovascular risk. Aliment Pharmacol Ther 2005;22 Suppl 2:28-30.
3. Sawant AM, Shetty D, Mankeshwar R, Ashavaid TF. Prevalence of dyslipidemia in young adult Indian population. J Assoc Physicians India 2008;56:99-102.
4. Kirtikar KR, Basu BD. Indian Medicinal Plants. International Book Distributers, Dehradun, India; 1990. p. 2724.
5. Joshi SG. Medicinal Plants. New Delhi: Oxford and IBH Publishing Co. Pvt. Ltd; 2004. p. 314.
6. Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. Heidelberg: Springer; 2007. p. 92.
7. Gogate VM. Ayurvedic pharmacology and therapeutic uses of medicinal plants (Dravyagunavignyan). Mumbai: Bharatiya Vidya Bhavan; 2008. p. 717.
8. Saravanan S, Subramaniam R, Subasini U, Victor RG, Govinda PD. Anti-hyperlipidemic and antioxidant potential of different fractions of Terminalia arjuna Roxb. Bark against PX-407 induced hyperlipidemia. Indian J Exp Biol 2011;49(4):282-88.
9. BASF Coroporation Pluronic® and Terronic® Surfactants, BASF Corporation, (Parsipanny, New Jersey); 1989. p. 1.
10. Gopal SR, Raymond JL. Automated enzymatic measurement of total cholesterol in serum. Clin Chem 1978;24:108-14.
11. Gopal SR, Raymond JL. Automated enzymatic measurement of total cholesterol in serum. Clin Chem 1978;24:108-14.
12. Burstein M, Scholnick HR, Morfin R. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J Lipid Res 1970;11:583-95.
13. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18:499–502.
14. Mertz DP. “Atherosclerosis-index” (LDL/HDL): risk indicator in lipid metabolism disorders. Med Klin 1980;75:159–61.
15. Malaspina JP, Bussière H, Le Calve G. The total cholesterol/HDL cholesterol ratio: a suitable atherogenesis index. Atherosclerosis 1981;40:373–75.
16. Kayamori F, Igarashi K. Effects of dietary nasunin on the serum cholesterol level in rats. Biosci Biotechnol Biochem 1994;58:570–1.
17. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978;2:302–10.
18. Anderson ME. Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol 1985;113:548–55.
19. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121–6.
20. Folch J, Lees M, Sloane Stanle GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957;226:497–509.
21. Wout ZG, Pec EA, Maggiore JA, Plicharla P, Williams RH, Johnston TP. Poloxamer 407-mediated changes in plasma cholesterol and triglycerides following intraperitonial injection to rats. J Par Sci Tech 1992;46(6):192-200.
22. Johnston TP, Palmer WK. Effect of Poloxamer-407 on activity of microsomal 3-hydroxy-3-methylglutaryl coA reducatase in rats. J Cardiovas Pharmacol 1997;29(5):580-5.
23. Goldstein JL, Brown MS. Regulation of mevalonate pathway. Nat 1990;343(6257):425-30.
24. Johnston TP. The P-407 induced murine model of dose-controlled hyperlipidemia and atherosclerosis: a review of findings to date. J Cardiovas Pharmacol 2004;43(4):595-606.
25. Myant NB, Mitropoulos KA. Choletserol 7α-hydroxylase. J Lip Res 1977;18(2):135-53.
26. Heuman DM, Vlahcevic ZR, Bailey ML, Hylemon PB. Regulation of bile acid synthesis II. Effect of bile acid feeding enzymes regulating hepatic cholesterol and bile acid synthesis in rat. Hepatol 1988;8(4):892-7.
27. Johnston TP, Palmer WK. Mechanism of Poloxamer-407 induced hypertriglyceridemia in rat. Biochem Pharmacol 1993;46(6):1037-42.
28. Navab M, Berliner JA, Watson AD, Hama SY, Territo MC, Lusis AJ, et al. The yin and yang of oxidation in the development of the fatty streak: a review based on the 1994 george lyman duff memorial lecture. Arteriosclerosis 1996;16:831-42.
29. O’Keefe Jr JH, Lavie Jr CJ, McCallister BD. Insights into the pathogenesis and prevention of coronary artery disease. Mayo Clin Proc 1995;70(1):69-79.
30. Rota S, McWilliam NA, Baglin TP, Byrne CD. Atherogenic lipoproteins support assembly of the prothrombinase complex and thrombin generation: modulation by oxidation and Vitamin E. Blood 1998;91(2):508-15.
31. Menno V, Adriaan GH, John JP, Jan AK. The HDL hypothesis: does high-density lipoprotein protect from atherosclerosis. J Lip Res 2010;51(8):2058-73.
32. Fielding CJ, Fielding PE. Cholesterol transport between cells and body fluids. Role of plasma lipoproteins and the plasma cholesterol esterification system. Med Clin North Am 1982;66(2):363-73.
33. Ohashi R, Mu H, Wang X, Yao Q, Chen C. Reverse cholesterol transport and cholesterol efflux in atherosclerosis. QJM 2005;98(12):845-56.
34. Philip B. The role of HDL-cholesterol in preventing atherosclerotic disease. Eur Heart J Suppl 2005;7:F4-F8.
35. Bandeali S, Farmer J. High density lipoprotein and atherosclerosis: the role of antioxidant activity. Curr Atheroscler Rep 2012;14(2):101-7.
36. Chapman MJ. Therapeutic elevation of HDL-cholesterol to prevent atherosclerosis and coronary heart disease. Pharmacol Ther 2006;111(3):893-908.
37. Podrez EA. Anti-oxidant properties of high-density lipoprotein and atherosclerosis. Clin Exp Pharmacol Physiol 2010;37(7):719-25.
38. Halliwell B. Mechanisms involved in the generation of free radicals. Pathol Biol 1996;44(1):6-13.
39. Rahman K. Studies of free radicals, antioxidants and co-factors. Clin Interv Aging 2007;2(2):219-36.
40. Lee KJ, Woo ER, Choi CY, Shin DW, Lee DG, You HJ, et al. Protective effect of Aceteoside on carbon tetrachloride-induced hepatotoxicity. Life Sci 2004;74(8):1051-64.
41. Tiwari AK. Natural product antioxidants and their therapeutic potential in mitigating peroxidative modification of lipoprotein and atherosclerosis: recent development. J Med Arom Plant Sci 1999;21:370-41.
42. Reed DJ. Glutathione: toxicological implications. Annu Rev Pharmacol Toxicol 1990;30:603-31.
43. Shan XO, Aw TY, Jones DP. Glutathione-dependent protection against oxidative injury. Pharmacol Ther 1990;47(1):61-71.
44. Parthasarathy S, Santanam N, Ramachandran S, Meilhac O. Oxidant and antioxidants in atherogenesis: an appraisal. J Lipid Res 1999;40(12):2143-57.
45. Martirosyan DM, Miroshnichenko LA, Kulakova SN, Pogojeva AV, Zoloedov VI. Amaranth oil application for coronary heart disease and hypertension. Lipids Health Dis 2007;6:1.
46. Frohilch J, Dobiásová M. Fractional esterification rate of cholesterol and ratio of triglycerides to HDL-cholesterol are powerful predictors of positive findings on coronary angiography. Clin Chem 2003;49(11):1873-80.
471 Views | 661 Downloads
How to Cite
Kaikini, A., S. Dhande, and V. Kadam. “ANTIHYPERLIPIDEMIC ACTIVITY OF METHANOLIC EXTRACT OF LEAVES OF BAMBUSA BAMBOS DRUCE AGAINST POLOXAMER-407 INDUCED HYPERLIPIDEMIA IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 2, Dec. 2014, pp. 393-8,
Original Article(s)