ANTIOXIDANT, PTP 1B INHIBITION AND Α-AMYLASE INHIBITION PROPERTY AND GC-MS ANALYSIS OF METHANOLIC LEAVES EXTRACT OF ACHYRANTHES ASPERA AND CATHARANTHUS ROSEUS OF NEPAL
Keywords:Achyranthes aspera, Catharanthus roseus, Antioxidant, Total phenolic content, Total flavonoid content, α-amylase inhibition, PTP1B inhibition, GC-MS
Objective: The present study was designed to study phytochemicals and biological activities of the methanolic extracts of two traditional medicine plants Achyranthes aspera and Catharanthus roseus of Nepalese origin.
Methods: Plant extracts were prepared by cold percolation method. Antioxidant activity, brine shrimp lethality assay, and analysis of phytochemical constituents were carried out using standard methods. The dinitro salicylic acid (DNS) method was used to study the inhibition effect of extracts on α-amylase enzyme. Furthermore, PTP 1B inhibitory activity was evaluated using p-nitrophenyl phosphate (p-NPP) as substrate.
Results: Phytochemical analysis showed the presence of phytochemicals like alkaloids, flavonoids, glycosides, reducing sugars, etc. in both plants. Brine shrimp lethality assay suggested the presence of pharmacologically active compounds. Total phenolic content and total flavonoid content of C. roseus were found to be higher with 73.21 mg GAE/g and 33.15 mg Q/g respectively than that of A. aspera, which was found to be 57.09 mg GAE/g and 28.96 mg Q/g respectively. Similarly, the α-amylase inhibition of A. aspera and C. roseus was found to be 97.60±1.11 µg/ml and 94.05±1.18 µg/ml comparative with IC50 68.13±0.46 µg/ml of standard acarbose. Protein tyrosine phosphatase 1B (PTP1B) inhibition showed IC50 for A. aspera and C. roseus to be 48.72±0.46 and 50.21±1.03 µg/ml, respectively. Qualitative GC-MS analysis of both plant hexane fractions showed acid and ester type of phytoconstituents.
Conclusion: These results suggested that both plants i. e A. aspera and C. roseus, Nepal origin showed biological activity by targeting multiple drug targets which justifies their traditional uses.
American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2009;32 Suppl 1:S62-7.
Gurzov EN, Stanley WJ, Brodnicki TC, Thomas HE. Protein tyrosine phosphatases: molecular switches in metabolism and diabetes. Trends Endocrinol Metabol 2014;15:1–10.
Alonso A, Sasin J, Bottini N, Friedberg I, Friedberg I, Osterman A, et al. Protein tyrosine phosphatases in the human genome. Cell 2004;117:699–711.
Hameed I, Masoodi SR, Mir SA, Nabi M, Ghazanfar K, Ganai BA. Type 2 diabetes mellitus: From a metabolic disorder to an inflammatory condition. World J Diabetes 2015;6:598-601.
Mbikay M. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: a review. Frontiers Pharm 2012;3:1–12.
Nguyen PH, Zhao BT, Ali MY, Choi JS, Rhyu DY, Min BS, et al. Insulin-mimetic selaginellins from Selaginella tamariscina with protein tyrosine phosphatase 1B (PTP1B) inhibitory activity. J Nat Prod 2015;78:34–42.
Sun J, Qu C, Wang Y, Huang H, Zhang M, Li H, et al. PTP1B, a potential target of type 2 diabetes mellitus. Mol Biol 2016;5:1–6.
Quang TH, Thi N, Ngan T, Yoon C, Cho K, Kang DG, et al. Protein tyrosine phosphatase 1B inhibitors from the roots of Cudrania tricuspidata. Molecules 2015;1:11173–83.
Chintoju N, Konduru P, Kathula RL, Remella R. Importance of natural products in modern history. Res Rev J Hosp Clin Pharm 2015;1:5–10.
Mishra JN, Verma NK. A brief study on Catharanthus roseus: a review. Int J Res Pharma Sci 2017;2:20–3.
Tamrakar AK, Maurya CK, Rai AK. PTP1B inhibitors for type 2 diabetes treatment: a patent review (2011-2014). Expert Opin Ther Pat 2014;24:1101–15.
Kafle B, Cho HJ. Isoxazolone derivatives as potent inhibitors of PTP1B. Bull Kor Chem Soc 2012;33:275-7.
Kunwar RM, Uprety Y, Burlakoti C, Chowdhary CL, Bussmann CW. Indigenous use and ethnopharmacology of medicinal plants in Far-West Nepal. Ethnobot Res Appl 2009;7:25-8.
Shrestha PM, Dhillion SS. Medicinal plant diversity and use in the highlands of dolakha district, Nepal. J Ethnopharmacol 2003;86:81-96.
Subba B, Srivastav C, Kandel RC. Scientific validation of medicinal plants used by Yakkha community of Chanuwa VDC, Dhankuta, Nepal. Springerplus 2016;5:155.
Srivastav S, Singh P, Mishra G, Jha KK, Khosa RL. Achyranthus aspera-an important medicinal plant: a review. J Nat Prod Plant Resour 2011;1:1–14.
Ghimire K, Banerjee, Gupta A, Dahal P. Phytochemical constituents and pharmacological uses of medicinal plant Achyranthes aspera: a review. World J Pharm Res 2014;4:470-89.
Priya CL, Kumar G, Karthik L, Rao KVB. Antioxidant activity of Achyranthes aspera Linn stem extracts. Pharmacologyonline 2010;2:228–37.
Edwin S, Jarald EE, Deb L, Jain A, Kinger H, Dutt KR, et al. Wound healing and antioxidant activity of Achyranthes aspera. Pharm Biol 2008;46:824–8.
Rani N, Sharma SK, Vasudeva N. Assessment of antiobesity potential of Achyranthes aspera linn. seed. J Evidence Based Complementary Altern Med 2012;2012:1-7.
Pereira DM, Valentão P, Sottomayor M, Ferreres F, Andrade PB. Phenolic Compounds in Catharanthus roseus. J Nat Products 2013;2:2093-106.
Tolambiya P, Mathur S. A study on potential phytopharmaceuticals assets in Catharanthus roseus L. (Alba). Int J Life Sci Biotechnol Pharma Res 2016;5:1–6.
Lalitha P, Jayanthi P. Preliminary studies on phytochemicals and antimicrobial activity of solvent extracts of Eichhornia crassipes (Mart.) Solms. Asian J Plant Sci Res 2012;2:115–22.
Pradeepa M, Kalidas V, Geetha N. Qualitative and quantitative phytochemical analysis and bactericidal activity of Pelargonium graveolens L’her. Int J Appl Pharm 2016;8:7-11.
Pisutthanan S, Plianbangchang P, Pisutthanan N, Ruanruay S, Muanrit O. Brine shrimp lethality activity of Thai medicinal plants in the family Meliaceae. Naresuan Univ J 2004;12:13–8.
Mobarak SA, Hossain S, Mia S. Microbicidal and cytotoxic actions of methanolic crude extracts of Ammannia multiflora-roxb. Int J Curr Pharm Sci 2015;7:54-6.
Meyer AS, Yi OS, Pearson DA, Waterhouse AL, Frankel EN. Inhibition of human low-density lipoprotein oxidation in relation to the composition of phenolic antioxidants in grapes (Vitis vinifera). J Agric Food Chem 1997;45:1638-43.
Arun N, Ragunathan MG, Jayanthi J. Antioxidant activity, total phenol, flavonoid, alkaloid, tannin, and saponin contents of leaf extracts of Salvinia molesta D. S. Mitchell. Asian J Pharm Clin Res 2016;9:185-8.
Paudel B, Bhattarai HD, Kim IC, Lee H, Sofronov R, Ivanova L, et al. Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from oymyakon region of the Republic of Sakha (Yakutia), Russia. Biol Res 2014;47:1–6.
Jebitta R, Allwin J. Antioxidant activity, total phenol, flavonoid, and anthocyanin contents of Jamun (Syzygium cumini) pulp powder. Asian J Pharm Clin Res 2016;9:361-3.
Bhutkar MA, Bhise SB. In vitro assay of alpha-amylase inhibitory activity of some indigenous plants. Int J Chem Sci 2012;10:457–62.
De Sales PM, de Souza PM, Simeoni LA, Magalhães P, de O, Silveira D. α-amylase inhibitors: a review of raw material and isolated compounds from plant source. J Pharm Pharm Sci 2012;15:141–83.
Na B, Nguyen PH, Zhao BT, Vo QH, Min BS, Woo MH. Protein tyrosine phosphatase 1B (PTP1B) inhibitory activity and glucosidase inhibitory activity of compounds isolated from Agrimonia pilosa. Pharm Biol 2016;54:474–80.
Kafle B, Aher NG, Khadka D, Park H, Cho H. Isoxazol-5(4H)one derivatives as PTP1B inhibitors showing an anti-obesity effect. Chem Asian J 2011;5:2073–9.
Patharajan S, BalaAbirami S. Antioxidant activity and phytochemical analysis of fractionated leaf extracts of Catharanthus roseus. Int J Pharm 2014;1:138–43.
Kamble A. Phytochemical studies on Achyranthes aspera. Sci Res J 2018;100:16–34.
Akhtar N, Ihsan-ul-Haq, Mirza B. Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species. Arab J Chem 2015;11:1223-35.
Hossain MJ, Khaleda L, Chowdhury MA, Arifuzzaman M, Al-Forkan. Phytochemical screening and evaluation of cytotoxicity and thrombolytic properties of Achyranthes aspera leaf extract. J Pharm Biol Sci 2013;6:30-8.
Aziz S, Saha K, Ahmed U, Parveen S, Ahmed N. Comparative studies on cytotoxic, antibacterial and free radical scavenging activity among different extracts of leaves and flowers of Catharanthus roseus available in Bangladesh. Int J Pharm Phytopharmacol Res 2014;4:169-72.
Kumar A, Singhal KC, Sharma RA, Vyas GK, Kumar V. Analysis of antioxidant activity of Catharanthus roseus L. and its association with habitat temperature. Asian J Exp Biol Sci 2013;4:155–8.
Pereira DM, Faria J, Gaspar L, Ferreres F, Valentão P, Sottomayor M, et al. Exploiting Catharanthus roseus roots: source of antioxidants. Food Chem 2010;121:56–61.
Inder M, Saggoo S. Screening of total phenol and flavonoid content in different cytotypes of two species of Achyranthes linn. from western Himalaya, India. Int J Pharm Pharm Sci 2017;9:205-10.
Rama P, Vignesh A, Lakshmanan G, Murugesan K. In vitro antioxidant activity of Achyranthes aspera linn. Int J Med Pharm Sci 2013;3:67–78.
Bhattecharjee D, Das A, Das SK, Chakraborthy GS. Clerodendrum infortunatum linn.: a review. J Adv Pharm Healthcare Res 2011;1:82-5.
Malathi V, Devi SS, Revathi K. Antidiabetic activity by the in vitro alpha-amylase and alpha-glucosidase inhibitory activity of Catharanthus roseus. Bioscan 2010;5:655–9.
Chattopadhyay RR. A comparative evaluation of some blood sugar lowering agents of plant origin. J Ethnopharmacol 1994;67:367-72.
Tolambiya P, Mathur. Identification of cost-effective drug through in silico approach of Catharanthus roseus plant ligand-receptor docking for type 2 diabetes. World J Pharm Pharm Sci 2017;6:1029-49.