ETHNOPHARMACOLOGICAL STUDY OF BRAIN OXIDATIVE STRESS IMPROVING POTENTIAL OF CURCUMIN IN INTOXICATED RATS
DOI:
https://doi.org/10.22159/ijpps.2021v13i5.40778Keywords:
Ethnopharmacology, Brain oxidative stress, CurcuminAbstract
Objective: The following study aimed to investigate the efficacy of curcumin at preventing amikacin neurotoxicity
Methods: Twenty-four male Wister albino rats were randomly divided into four groups including-G (1): control group includes six rats, they were administered 0.5 ml of saline orally for 14 consecutive days. G (2): includes six rats; they were administered 200 mg/kg curcumin orally for 14 consecutive days. G (3): includes six rats, they were administered 300 mg/kg body weight/day of amikacin intraperitoneally for 14 consecutive days G (4): includes six rats, they were administered 200 mg/kg curcumin orally concurrently with 300 mg/kg body weight/day of amikacin. All animals were kept in the same conditions from feed, heat and humidity.
Results: According to the result obtained after sacrification of all animals after the end of 14 d, Results revealed that amikacin at the dose rate of 300 mg/kg b. wt for 14 d induces significant changes in oxidative stress markers compared to the control group, a significant reduction in CAT. SOD. GSH (1.51±0.16, 77.00±0.73 and 84.06±4.42) respectively compared to control (3.63±0.11, 98.48±0.18 and 117.05±0.52) along with a significant increase in MDA activity (219.02±3.34) compared to control group (180.42±0.19), That indicate oxidative stress effect of it. On the beneficial side rats received amikacin 300 mg/kg B. wt I/p concurrently with 200 mg/kg b. wt curcumin for successive 14day result in a significant increase in CAT. SOD. GSH (2.23±0.09,92.00±0.26, 102.25±1.71) and decrease in MDA concentration (139.23±3.89) compared to amikacin treated group levels along with histopathological changes appear in brain tissue in the group treated with amikacin include nuclear pyknosis and degeneration in some neurons in the hippocampus, multiple focal eosinophilic plaque formation in the striatum also this results enhanced by activated caspase-3 expression in the brain tissue following amikacin administration.
Conclusion: The present study proved that Oral administration of curcumin at the dose of 200 mg/kg for 14 d concurrently with amikacin significantly mitigates its neurotoxic and oxidative stress effects.
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References
Cunha BA. Aminoglycosides: current role in antimicrobial therapy. Pharmacotherapy 1988;8:334-50.
Gilbert DN, Mandell GL, Bennett JE, Dolin R. Aminoglycosides in principles and practice of infectious diseases 2000;5:307–36.
Cipullo JP, Burdmann EP. Aminoglycoside nephrotoxicity. Braz J Cardiovasc Sur 2006;21:444-52.
Deliana NI R, Melva I, Vivian S. Effects of curcumin and nanocurcumin on cisplatin-induced nephrotoxicity in rat: copper transporter 1 and organic cation transporter 2 as drug. Transporters Int J Appl Pharm 2018;1:172.
Ak T, Gulcin I. Antioxidant and redical scavening properties of curcumin. Chem Boil Interact 2008;174:27-28.
Chan WH, Wu HJ. Antiopoptotic effects of curcumin on photosensitized human epidermal carcinoma A431 cells. J Cell Biochem 2004;92:200-12.
Aktas C, Kanter M, Erboga M, Oztrul S. Antiopoptotic effects of curcumin on cadmium induced opoptosis in rat testes. Toxicol Ind Health 2012;28:122-30.
Ghosh S, Banerjee S, Sil PC. The beneficial role of curcumin on inflammation, diabetes and neurodegenerative disease: a recent update. Food Chem Toxicol 2015;83:111-24.
Batoo AS, Hussain K, Singh R, Sultana M, Sharma N, Nabi B, et al. Biochemical and oxidative alterations induced by acute amikacin toxicity in albino wistar rats. J Anim Res 2018;8:407-10.
Ali BH, AlWabel N, Mahmoud O, Mousa HM, Hashad M. Curcumin has a palliative action on gentamicin‐induced nephrotoxicity in rats. Fundam Clin Pharmacol 2005;19:473-7.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61:882–8.
Nishikimi M, Roa N, Yogi K. Measurement of superoxide dismutase Biochem Biophys Res Common 1972;46:849-54.
Banchroft JD, Stevens A, Turner DR. Theory and practice of histological techniques. Fourth Ed. Churchil Livingstone, New York: London, San Francisco, Tokyo; 1996.
Mohamed AA, Galal AA, Elewa YH. Comparative protective effects of royal jelly and cod liver oil against neurotoxic impact of tartrzaine on male rats pups brain. Acta histochemica 2015;117:649-58.
Hanai H, Sugmioto K. Curcumin has bright prosoects for the treatment of inflammatory bowl diseases. Cur Pharm Des 2009;15:2087-94.
Uger S, Ulu R, Dogukan A, Gurel A, Yigit IP, Gozel N, et al.: The renoprotective effect of curcumin in cisplatin induced nephrotoxicity. Ren Fail 2015;37:332-6.
Khatri DK, Juvekar AR. Neuroprotective effect of curcumin as evinced by abrogation of rotenone-induced motor deficits, oxidative and mitochondrial dysfunctions in mouse model of Parkinson's disease. Pharmacol Biochem Behav 2016;150:39-47.
Weydert CG, Cullen JJ: Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissues. Natural Protocola 2010;5:51-66
Masellia RM, Dibendetto R, Vari R, Filesi CM, Giovannini C. Noval mechanism of natural antioxidant compounant in biological system: involvement of glutathione and glutathione related enzymes. J Nut Biochem 2005;16:577-86.
Davey MW, Stals E, Panis B, Keulemans J, Swennen RL. High-throughput determination of malondialdehyde in plant tissues. Anal Biochem 2005;347:201-7.
Pryor WA, Stanley JP. Letter: a suggested mechanism for the production of malonaldehyde during the autoxidation of polyunsaturated fatty acids. Non enzymatic production of prostaglandin end peroxides during autoxidation. J Org Chem 1975;40:3615-7.
Farmer EE, Davoine C. Reactive electrophile species. Curr Opin Plant Biol 2007;10:380-6.
Zelko IN, Mariani TJ, Folz RJ. Superoxide dismutase multigene family: a comparison of the CuZn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression. Free Radical Biol Med 2002;33:337-49.
Cole GM, Teter B, Frautschy SA. Neuroprotective effects of curcumin. Adv Exp Med Biol 2007;595:197-212.
Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J 2012;5:9-19.
Deavall DG, Martin EA, Horner JM, Roberts R. Drug-induced oxidative stress and toxicity. J Toxicol 2012;2012:645460.
Pereira CV, Nadanaciva S, Oliveira PJ, Will Y. The contribution of oxidative stress to drug-induced organ toxicity and its detection in vitro and in vivo. Expet Opin Drug Metabol Toxicol 2012;8:219-37.
Singhal M, Prajapati B. In vivo evaluation of aminoglycoside induced nephrotoxicity and hepatotoxicity in albino rats. Pharmacologyonline 2011;2:451-7.
Joe B, lokesh BR. Role of capacicum, curcumine and dietry n-3 fatty acid in lowering the generation of reactive oxygen species in rat peritoneal macrophage. Biochim Biophys Acta 1994;1224:255-63.
Susan M, Rao MN. Induction of glutathione S-transferase activity by curcumin in mice. Arzneimittel Forschung 1992;42:962-4.
Jagetia GC, Rajanikant GK. Curcumin stimulates the antioxidant mechanisms in Mouse skin exposed to fractionated γ-irradiation. Antioxidants 2015;4:25–41.
Mendez A, Nava Ruiz C, Juarez D, Rodriguz E, Gomez PY. Oxidative stress assoiated with neuroopoptosis in experimental model of epilepsy. Oxid Med Cell Longev 2014. DOI:10.1155/2014/293689.
Agostinia M, Tucci P, Melino G. Cell death pathology: perspective for human diseases. Biochem Biophys Res Commun 2011;414:451–5.
Porter AG, Janick RU. Emerging roles of caspase 3 in opoptosis. Cell Death Differ 1999;6:99-104.
Faubel S, Ljubanovic D, Reznikov L, Somerset H, Dinarello CA, Edelstein CL. Caspase-1-deficient mice are protected against cisplatin-induced apoptosis and acute tubular necrosis. Kidney Int 2004;66:2202-13.
Piwocka K, Jargua E, Skierski J, Gradaska L, Sikora E. Effect of glutathione depletion on caspase 3 independent opoptosis pathway induced by curcumin in jurkat cell. Free Radical Biol Med 2001;31:670-8.
Park E, Chun HS. Protective effect of curcumin on manganese induced BV-2 microglial cell death. Biol Pharm Bull 2017;40:1275-81.
