ETHNOPHARMACOLOGICAL STUDY OF BRAIN OXIDATIVE STRESS IMPROVING POTEINTIAL OF CURCUMIN IN INTOXICATED RATS

Abstract

Objective: As of now antibiotics play a great rule in treatment of several  bacterial, fungal and parasitic infection , However numerous adverse effects of antibiotics open the window for using natural products and the last century has seen a big development in the application and usage of natural product therapeutically ,So the following study aimed to investigate the adverse effect of amikacin in oxidative status of brain tissue beside histological and immunohistochemical  examination of brain tissue as well as the neuroprotective effect  of curcumin.

Methods ,Tweenty four  male Wister albino rats were randomly divided into four groups including :- G (1) : control group includes six rats ,they were adminstered 0.5ml of saline orally for 14 consecutive days . G (2):  includes six rats ,they were administered 200 mg/kg curcumin orally for 14 consecutive days . G (3): includes six rats ,they were adminstered 300 mg/kg body weight/day of amikacin intraperitonially for 14 consecutive days  G (4): includes six rats ,they were administered 200mg/kg curcumin orally concurrently with 300 mg/kg body weight/day of amikacin.All animals were kept  in the same conditions .

Results, According to the result obtained after sacrification of all animals after the and of 14 days, Results revealed that amikacin induced marked increase in malondialdehyde (MDA) and decreases in glutathione (GSH) and catalase (CAT),Superoxide dismutase (SOD) levels that indicate oxidative stress levels along with histopatholcgical changes appear in brain tissue in group treated with amikacin include nuclear pyknosis and degeration in some neurons in hippocampus , multiple focal eosinophilic plague formation in the striatum also this results   enhanced by activated caspase-3 expression in the brain tissue following amikacin adminsteration .On the benifacial side, oral adminsteration of curcumin at the dose 200 mg/kg for 14 days concurrently with amikacin significantly  mitigate theses toxic effects.