Urine Metformin method

  • Mary Rebecca Y ICMR- National Institute for Research in Tuberculosis, Chennai, India
  • Sudha V ICMR- National Institute for Research in Tuberculosis, Chennai, India
  • Vijayakumar A ICMR- National Institute for Research in Tuberculosis, Chennai, India
  • Hemanth Kumar A K ICMR-National Institute for Research in Tuberculosis


Objective: To develop and validate an easy and sensitive HPLC method for quantitation of metformin in urine.

Materials and Methods: The technique involved deproteinisation of urine sample with methanol and analysis of the supernatant the usage of Zorbax 300 – SCX, 4.6 X 150mm ID, 5 µm particle size  and UV detection at a wavelength of 233 nm.

Results: The assay was specific for Metformin and linear from 1.25 to 50.0μg/ml. The relative well known deviation of intra- and inter-day assays was lesser than 7%. The recovery of Metformin from urine ranged from 97- 103%.

Conclusions: An easy and sensitive HPLC approach for quantitation of Metformin in urine has been developed. Due to its simplicity in sample preparation and instrumentation, this technique can be used for pharmacokinetic studies of metformin in urine samples.


Keywords: HPLC method – Metformin in urine – Anti- diabetic drug - Pharmacokinetics


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Author Biography

Hemanth Kumar A K, ICMR-National Institute for Research in Tuberculosis

Clinical Pharmacology,

Scientist "D"


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How to Cite
Y, M. R., S. V, V. A, and H. K. A K. “QUANTITATION OF METFORMIN IN URINE BY RP-HPLC METHOD AND ITS APPLICATION IN PHARMACOKINETICS: Urine Metformin Method”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 13, no. 5, Apr. 2021, doi:10.22159/ijpps.2021v13i5.41051.
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