PROTECTIVE EFFECT OF MORINGA OLEIFERA SEED EXTRACT ON CISPLATIN INDUCED NEPHROTOXICITY IN RATS

  • Faten El sayed Faculty of veterinary medicine ,Benha unversity

Abstract

Objective: the main objective of this study is to investigated the antioxidant and nephroprotective efficacy of moringa oleifera seed extract (MOSE) against cisplatin which induced acute renal injury.


Methods: Forty male Wister rats were equally segregated into 4 groups (10 rats per group): group I (0.5 ml of sterile saline orally), group II (200 mg MOSE/kg b.wt orally for 10 consecutive days),  group III (7.5 mg cisplatin/kg b.wt/ intraperitonially as a single dose on the 5th day of the experiment) and group IV (200 mg MOSE/kg orally  for 10 days followed by  7.5 mg cisplatin/kg body weight/ intraperitonially once as a single dose on the 5th day of the experiment. Serum biochemical analysis of renal biomarkers (urea, uric acid, and creatinine), oxidative stress markers (malondialdehyde [MDA]), a crucial antioxidant enzyme (catalase) and the expression of renal activity interleukin (IL)-6, (IL)-10 and TNF-α mRNA were determined. Histopathological examination of renal tissue was done.


Results: Cisplatin induced renal damage, increased renal biomarkers (urea, creatinine and uric acid)(375.87±1.65, 5.238±0.25, 4.47±0.25). tissue concentrations of malondialdehyde, IL-6 and TNF-α.( 387.56±0.97, 2.188±0.20, 3.06±0.27)compared to control group( 140.58±1.25,0.938±0.017, 1.24±0.17), ( 163.99±1.34, 1.008±0.05, 0.982±0.026) Moreover, cisplatin induced significantly down-regulation of anti-inflammatory (IL-10) and catalase (0.780±0.47, 1.62±0.06) compared to control one ( 1.010±0.02, 3.12±0.11), . The histopathological examination showed renal tissue damage and degeneration of tubules in the cortical portion in cisplatin group. However,interestingly concurrent adminsteration of the MOSE with cisplatin can  alleviated the renal damage, oxidative stress and renal toxicity caused by cisplatin.


Conclusion:These results suggest that the antioxidant and the anti-inflammatory effects of MOSE alleviate the cisplatin-induced nephrotoxicity.

Keywords: Cisplatin, Moringa Oleifera, Oxidative stress, Nephrotoxicity, Cytokines

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El sayed, F. “PROTECTIVE EFFECT OF MORINGA OLEIFERA SEED EXTRACT ON CISPLATIN INDUCED NEPHROTOXICITY IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 13, no. 5, Apr. 2021, doi:10.22159/ijpps.2021v13i5.41125.
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