A OPTIMIZATION AND CHARACTERIZATION OF DOXORUBICIN LOADED SOLID LIPID NANOSUSPENSION FOR NOSE TO BRAIN DELIVERY USING DESIGN EXPERT SOFTWARE
Brain Targeting of Doxorubicin SLNs Via Nasal Route
Objective: The goal of the current study was to investigate the possible use of solid lipid nanosuspension (SLNs) as a drug delivery method to boost doxorubicin (DOX) brain-targeting performance after intranasal (i.n.) administration.
Method: 33 factorial design was iapplied for optimization by using lipid concentration, surfactant concentration and High-speed homogenizer (HSH) stirring time as dependent variables and their effect were observed on particles size, Polydispersity index (PDI) and entrapment efficiency.
Result: With the composition of Compritol® 888 ATO (4.6 % w/v), tween 80 (1.9 % w/v) and HSH stirring time, the optimized formula DOX-SLNs prepared (10 min). Particle isize, PDI, zeta ipotential, entrapment efficiency, percent in vitro release, were found to be 167.47 ± 6.09 nm, 0.23 ± 0.02, 24.1 mV, 75.3 ± 2.79, and 89.35 ± 3.27 percent in 24 h, respectively, for optimized formulation (V-O). No major changes in particle size, zeta potential, and entrapping efficiency were found in stability studies at 4 ± 2 °C (refrigerator) and 25 ± 2 °C/60 ± 5% RH up to 3 months.
Conclusion: Following the non-invasive nose-to-brain drug delivery, which is a promising therapeutic strategy, the positive findings confirmed the current optimized DOX-loaded SLNs formulation.
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