DETERMINATION OF PHYSICOCHEMICAL PARAMETERS AND ANTIOXIDANT PROPERTIES OF RHUS JAVANICA LINN.
Objective: The present work presents determination of physicochemical parameters of the leaves and the antioxidant properties of methanolic extract of leaves, fruits and bark of Rhus javanica L.
Methods: The leaves of Rhus javanica L. was used for the determination of physicochemical parameters using the standard protocol. The leaves, fruits and bark of the plant were extracted successively with petroleum ether, chloroform and methanol. The methanolic extracts were used to determine antioxidant properties. DPPH, hydrogen peroxide and hydroxyl radical scavenging activities were determine using methods described by Blois (1958), Rutch et al. (1989) and Halliwell (1995) respectively. The reducing power was determined by the method described by Oyaizu (1986). Total phenols and flavonoids were analyzed using Folin-Ciocalteu’s and aluminum chloride method respectively.
Results: The moisture content, extractive value (alcohol and water), total ash, acid insoluble ash and water soluble ash were 0.29%, 24.502%, 19.314%, 8.2%, 0.45% and 4.75% respectively. The fruit extract exhibit the highest scavenging activity presented as percentage inhibition, 75.00 ± 0.89% (100 μg/ml) for DPPH free radical and 74.7 ± 0.68% (100 μg/ml) for hydrogen peroxide radical. The standard BHT (100 μg/ml) shows 78.5 ± 0.43% inhibition for DPPH free radical and 82.5 ± 0.50% inhibition for hydrogen peroxide free radical. The leaves show the highest hydroxyl radical scavenging activity, 65.00 ± 0.40% (100 μg/ml) while the standard Ascorbic acid exhibit 73.81 ± 0.61% (100 μg/ml) inhibition. The highest reducing power was shown by the bark extract but lower than the standard ascorbic acid. The bark extract shows the highest phenolic and flavonoid content 49.86 mg GAE/g and 19.38 mg QE/g respectively of the dry weight of the extract.
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