• Lavanya R sri Ramachandra university
  • Seethalakshmi S Sri Ramachandra University
  • Gopal V ESIC Medical College & PGIMSR, Chennai
  • Chamundeeswari D Sri Ramachandra University


Sulphated polysaccharide, PMNL cells, Nil, TPA


Objective: The present study aims to evaluate the anti-inflammatory activity of crude sulphated polysaccharide from marine brown algae sargassum ilicifolium

Methods: The present study involves the investigation of crude sulphated polysaccharide from marine brown algae sargassum ilicifolium for anti-inflammatory activity by TPA [12- O- Techanoyl 13 – Myristate] induced inflammation in Polymorphonuclear leukocytes [PMNL]. T.h.e cell viability by Trypan blue dye exclusion assay, MTT assay, release of Cathepsin D, Nitrite, TNF-α levels was assessed. Diclofenac was used as the standard drug for comparison.

Results: Crude sulphated polysaccharide were isolated from brown seaweed sargassum ilicifolium. Crude sulphated polysaccharide showed the significant increase in the number of viable PMNL cells. It has further reduced the release of Cathepsin D, Nitrite and TNF-α. The levels of which are increased during inflammatory conditions.

Conclusion: Therefore our studies support the isolation and the use of sulphated polysaccharide in treating inflammation and rheumatism.



Download data is not yet available.

Author Biography

Lavanya R, sri Ramachandra university


Department of Pharmacology



Park PJ, Heo SJ, Park EJ, Kim SK, Byun HG, Jeon BT, et al. Reactive oxygen scavenging effect of enzymatic extracts from Sargassum thunbergii. J Agric Food Chem 2005;53:6666-72.

Hu-Xue C, Fang Y, Lin H, Chen L, Li ZJ, Deng D, et al. Chemical characters and antioxidative properties of sulfated polysaccharides from Laminaria japonica. J Appl Phycol 2001;13:67-70.

Raghavendran HR, Sathivel A, Devagi T. Hepatoprotective natureof seaweed alcoholic extract on acetaminophen induced hepatic oxidative stress. J Health Sci 2004;50:42-6.

Wong CK, Ooi VE, Ang PO. Protective effects of seaweeds against liver injury caused by carbon tetrachloride in rats. Chemosphere 2000;41:173-6.

Wajant H, Pfizenmaier K, Scheurich P. Tumor necrosis factor signaling. Cell Death Differ 2003;10:45–65.

Tako M. Rheological characteristics of fucoidan isolated from commercially cultured Cladosiphon okamu-ranus. Bot Mar 2003;46:461–5.

Chamundeeswari D. Modulation of inflammatory markers by corallocarpus epigaeus tubers in TPA induced poly morpho nuclear leukocytes. Bio Med 2010:30(2):176-82.

De Chatelet LR, Shirley P, Johnston RB Jr. Effect of phorbol myristate acetate on the oxidative metabolism of human polymorphonuclear leukocytes. Blood 197;647:545-54.

Freshney RI. Culture of animal cells: a manual of basic technique. 3rd Ed. Wiley-Liss: New York USA; 1994. p. 475-90.

Warren strobe. Trypan blue exclusion test of cell viability curr protoc immunol john and wiley sons May Appendix 3B; 2001.

Wilson AP. Cytotoxicity and viability assays in animal cell culture: a practical approach, 3rd ed. Oxford University Press: Oxford; 2000. p. 175-219.

Mosmann T. Rapid colrimetric assay for cellular growth and survival application to proliferation and cytotoxicity assays, J Immunol Methods 1983;65(1-2):55-63.

Barrett AJ, Cathepsin D. Purification of isoenzymes from human and chicken liver. Biochem J 1970;117:601-7.

Palmer RMJ, Femge AG, Moncada S. Nitric oxide release accounts for the biological activity of endothelial derived relaxing factor. Nat 1987;327:524-6.

Young-Mi Park, Jong-Heon Won, Kyung-Jin Yun. Preventive effect of ginkgo biloba extract (GBB) on the lipopolysaccharide-induced expressions of inducible nitric oxide synthase and cyclooxygenase-2 via suppression of nuclear factor-κB in RAW 264.7 Cells. Biol Pharm Bull 2006;29(5):985-90.

Aggarwal BB, Reddy S. Tumor necrosis factor. In: Nicola, NA. ed. Guidebook to Cytokines and their receptors. A Sambrook and Tooze Publication of Oxford: University Press; 1994. p. 103-4.



How to Cite

R, L., S. S, G. V, and C. D. “EFFECT OF CRUDE SULPHATED POLYSACCHARIDE FROM MARINE BROWN ALGAE IN TPA INDUCED INFLAMMATION ON POLY MORPHONUCLEAR LEUKOCYTES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 100-2, https://innovareacademics.in/journals/index.php/ijpps/article/view/4141.



Original Article(s)