GENETIC BARCODING AND BOTANICAL STUDIES ON POTHOS SCANDENS L. LEAVES-AN EXTRA-PHARMACOPOEIAL DRUG OF AYURVEDA
Keywords:Analytical, Anukta dravya, Genetic barcoding, Pothos scandens, Wound healing
Objective: This study was undertaken to carry out genetic barcoding, pharmacognostical and phytochemical studies on leaves of Pothos scandens L. belonging to the family Araceae.
Methods: P. scandens, was collected from Cochin, Kerala, during the month of August 2019. Its leaves were examined for pharmacognostical characters and physicochemical, qualitative analysis and HPTLC studies was carried out following standard protocols recommended by the Ayurvedic Pharmacopoeia of India. Genetic barcoding of the fresh tender leaves samples was carried out through following standard methods.
Results: Leaves are simple, alternate, broadly winged petiole. Transverse section through winged petiole portion showed U-shaped, epidermal cell along with cluster crystals. Transverse section through midrib boat-shaped structure, mesophyll undifferentiated, consist parenchyma cell, epidermis with thick cuticle along with waxy coating. Leaves powder shows green in color and microscopy reveals the presence of prismatic crystals, raphides, fibres, acicular crystals and monocot type 1 stomata. The plant is identified as Pothos scandens based on sequence homology and phylogenic analysis. Qualitative results show the presence of carbohydrates, protein, alkaloids and tannins. HPTLC profile shows 7 spots at both short UV and long UV and ferulic acid and kaempferol were identified through quantitative HPTLC.
Conclusion: Pothos scandens is a climber with simple, alternate leaves. Acicular crystals and raphides, mucilaginous cavities, undifferentiated mesophyll are key characters of the identification of its leaf. DNA Barcoding results helps in correct identification of Pothos scandens. The results obtained from the phytochemical study will be helpful in the determination of the strength of purity and also for further standardization of the plant.
Anonymous. Macroscopic and microscopic examination: Quality control methods for medicinal plant materials. WHO. Geneva; 1998.
Vinayaka KS, Hegde SV, Banakar S, Kekuda TRP. Antifungal potency of Gnidia glauca (Fresen) gilg and Pothos scandens L. Nat Prod 2009;5:146-8.
Dass PS, Choudhary MD, Dutta BK. Flora of barak valley assam. Regency Publication 2013;1:423.
Jayaweera DMA. Medicinal plants (Indigenous and Exotic) used in Ceylon. Part 1. The national science council of Sri Lanka Colombo; 1981. p. 139.
Saurabh Gupta, Shareen Singh, Renu Gupta, Thakur Gurjeet Singh. Pharmacological and phytochemical updates on Pothos scandens L. Pharmacogn Commn 2018;8:138-45.
Sojeetra N, Acharya RN. A review on ethnomedicinal claims and spread of Pothos scandens L. Eur J Med Plants 2020;31:22-8.
Mohan. Pharmacognostical and phytochemical studies on Pothos scandens L. Int J Phytomed 2010;2:277-83.
Trease, Evans WC. Pharmacognosy. 16th ed. London: Saunders Elsevier; 2009. p. 544-5.
Trease, Evans WC. Pharmacognosy. 16th ed. London: Saunders Elsevier; 2009. p. 567-56.
Khandelwal KR. Practical pharmacognosy. 19th ed. Pune: Nirali Prakashan; 2008. p. 9-19.
Wallis TE. Text book of pharmacognosy. 5th ed. New Delhi: CBS Publishers and Distributors; 2002. p. 578-81.
Kokate CK. Practical pharmacognosy. 4th ed. Vallabh Prakashan: Delhi; 1994.
Trease, Evans WC. Pharmacognosy. 16th ed. Saunders Elsevier. London; 2009. p. 569.
Richards E, Reichardt M, Rogers S. Preparation of genomic DNA from plant tissue. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K. (Eds). Curr Protoc Mol Biol, John Wiley and Sons, New York, USA; 1994.
Rasmussen HN, Rasmussen OF, Andersen JK, Olsen JE. Specific detection of pathogenic yersinia enterocolitica by two-step PCR using hot-start and DMSO. Mol Cell Probes 1994;8:99–108.
Kress WJ. Plant DNA barcodes and a community phylogeny of a tropical forest dynamics plot in Panama. Proc Natl Acad Sci USA 2009;106:18621–6.
Levin RA. Family-level relationships of onagraceae based on chloroplast rbcL and ndhF data. Am J Bot 2003;90:107–15.
Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 1999;41:95–8.
Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version for bigger data sets. Mol Biol Evol 2016;33:1870–4.
Anonymous. The ayurvedic pharmacopoeia of India. Part II. Vol. II. 1st ed. New Delhi: Government of India, Ministry of Health and Family Welfare, Department of AYUSH; 2008.
Khandelwal KR. Practical pharmacognosy. 19th ed. Pune: Nirali Prakashan; 2008. p. 149-53.
Egon S. Thin layer chromatography. Springer Publication; 1969.
Dinesh Kumar. Macroscopical and microscopical evaluation of leaves of Clerodendrum inerme Gaertn. Int J Biol Med Res 2011;2:404-8.
Gamble JS. Flora of the presidency of madras. Vol 1. Dehradun. Bishen Singh Mahendrapal Singh; 2011. p. 1591.
Rao RS, Ramayya N. Trichome types and their taxonomic importance in the Tiliaceae. Indian J Bot 1987;10:65–73.
Trease GE, Evans WC. Pharmacognosy. 12th ed. London: English Language Book Society/Bailliere Tindall; 1983.
Anonymous. Quality assurance pharmaceuticals: a compendium of guidelines and related materials. good manufacturing practices and inspection. Geneva: WHO; 1996. p. 2.
Anonymous. African Pharmacopoeia. General methods for Analysis. OAU/STRC Scientific Publications. Lagos 1986;2:1-5, 137-49, 223-37.
Baravalia Y, Nagani K, Chanda S. Evaluation of pharmacognostic and physicochemical parameters of Woodfordia fruticosa Kurz. Flowers. Pharmacogn J 2011;2:13-8.
Mallikharjuna PB, Rajanna LN, Seetharam YN, Sharanabasappa GK. Phytochemical studies of Strychonos potatorum L. F. A medicinal plant. E J Chem 2007;4:510-8.
Balabhaskar R, Vijayalakshmi K. High-performance thin-layer chromatography fingerprint profile of Bauhinia tomentosa Linn. leaves. Asian J Pharm Clin Res 2018;11:344-9.
Graf E. Antioxidant potential of ferulic acid. Free Radical Biol Med 1992;13:435–48.
Bezerra G. Compatibility study between ferulic acid and excipients used in cosmetic formulations by TG/DTG, DSC and FTIR. J Ther Anal Calorim 2017;127:1683–91.
Sak K. Cytotoxicity of dietary flavonoids on different human cancer types. Pharmacogn Rev 2014;8:122-46.
Calderon Montano. Review on the dietary flavonoid kaempferol. Mini Rev Med Chem 2011;11:298–344.
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