• SHWETA R. GOPHANE School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 431606 India
  • SAGAR R. JADHAO School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 431606 India
  • PREETI B. JAMDHADE School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 431606 India



Flavonoids, GC-MS, ADME, Glycolate oxidase


Objective: Bergenia ciliata (family-Saxifragaceae) is a well-known herb for kidney stone. The main objective of the study was the identification of flavonoids along with ADME profile. Another supportive objective was to check inhibition of enzymes which perform active role in oxalate synthesis.

Methods: The hydromethanolic extract was fractionated by liquid-liquid extraction to obtain ethyl acetate and ethyl ether fractions. The chemical structures of the purified compounds were identified by gas chromatography-mass spectrometry.

Results: A total of 12 volatile chemical compounds belonging to hydrocarbons, esters, alcohols, fatty acids, ketones, etc. were identified and characterized in ethyl acetate fraction through GC-MS analysis Fractions enriched in flavonoids showed glycolate oxidase and lactate dehydrogenase enzyme inhibition with IC50 value (µg/ml) 65.76 and 69.84 respectively. The kinetic behaviour of the extracts that inhibit the Glycolate oxidase and Lactate dehydrogenase activity was determined by the Lineweaver-Burk plot. The mode of inhibition of the studied plant extract was type of a non-competitive inhibition. ADMET screening of compounds successfully passed all the parameters of screening.

Conclusion: On the basis of the results, it was found that Bergenia ciliata (rhizome) may serve as a novel and rich source of therapeutic compounds and it can be further explored for urolithiasis treatment purposes.


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How to Cite

GOPHANE, S. R., S. R. JADHAO, and P. B. JAMDHADE. “BERGENIA CILIATA: ISOLATION OF ACTIVE FLAVONOIDS, GC-MS ANALYSIS, ADME STUDY AND INHIBITION ACTIVITY OF OXALATE SYNTHESIZING ENZYMES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 13, no. 11, Nov. 2021, pp. 34-46, doi:10.22159/ijpps.2021v13i11.42127.



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