ANTI-PROSTATE AND COLON CANCER AND DIURETIC EFFECTS OF QURANI PLANTS' MIXTURE (A NEW PHARMACEUTICAL PRODUCT)
Keywords:Cytotoxicity, Colon cancer, Prostate cancer, Edible and medicinal plants, Nil, Diuretic activity, Urine analyses, Kidney functions
Objective: QURANI plants' mixture is a new pharmaceutical product composed of some edible and medicinal plants (15 plants) mentioned in the Holy Quran (in a certain percentage, according to that is mentioned in Patent no. 1429/2013, presented to the Academy of Scientific Research and Technology, Egypt in 11/9/2013). The main aim of this work is to determine the cytotoxic effect of this extract against both human prostate (PC3) and colon (HCT116) cancer cell lines and also to evaluate the diuretic effect of this extract.
Methods: In vitro evaluation of the ethanolic extract of QURANI plants' mixture regarding its cytotoxic effect against both human prostate (PC3) and colon (HCT116) cancer cell lines was carried out. In vivo studies of the diuretic effect of daily feeding rats with 2, 4 and 8 g/kg of QURANI plants' mixture was performed also.
Results: In vitro studies of the ethanolic extract of QURANI plants' mixture revealed the cytotoxic effect of this extract against both human prostate (PC3) and colon (HCT116) cancer cell lines. Inhibition caused by using 100 Âµl/ml (this volume is related to 25 Âµg d. w. of QURANI plants' mixture) of this extract reached to 36.300Â±0.085 and 76.300Â±0.045 against HCT116 and PC3 cell lines, respectively. IC50 of this extract against PC3 cell line equals 44.60 Âµl/ml (this volume is related to 11.15 Âµg d. w. of QURANI plants' mixture), but it can not be detected in case of HCT116 cell line using these investigated concentrations of this extract. In vivo studies of the diuretic effect of daily feeding rats with 2, 4 and 8 g/kg of QURANI plants' mixture showed that, the amount of urine increased by increasing the amount of this mixture when compared to the control group. Results of kidney functions and urine analyses revealed that, this mixture has not any bad side effects in this regard, moreover, rats feeding with QURANI plants' mixture have better kidney functions (urea and creatinene are lower in case of rats feeding with QURANI plants' mixture than those of control group). Rats fed with this mixture are also having normal urine physical and chemical characters compared to the control group.
Conclusion: Results of anticancer and diuretic effects of QURANI plants' mixture will lead us to more biological and chemical investigations of this new, cheap and safe pharmaceutical natural product.
The holy Quran.
Eman A Alam. Patent no: 1429/2013 (A new Pharmaceutical Product from Plants Mentioned in the Holy Quran), presented. Acad Sci Res Technol Egypt 11/9/2013.
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