MANGO SEEDS: A POTENTIAL SOURCE FOR THE ISOLATION OF BIOACTIVE COMPOUNDS WITH ANTI-CANCER ACTIVITY

  • Bibechana Timsina Jain University
  • Varalakshmi Kilingar Nadumane Department of Biotechnology, Center for Post-Graduate Studies, Jain University, 18/3, 9th Main, III Block, Jayanagar, Bangalore 560011 Karnataka State, India

Abstract

Objective: The mango (Mangifera indica L.), is a fruit with high levels of phytochemicals and the seeds have antioxidant, anti-inflammatory and anti-obesity effects. This study aims at the extraction of metabolites from mango seeds and evaluation of the antiproliferative properties on cancer cell lines.

Methods: The antiproliferative effects of the ethanol extract of mango seeds were evaluated on the cancer cell line HeLa, CHO cell lines and also on normal human lymphocytes by MTT assay. The extract was purified by TLC and characterized by LC-MS methods.

Results: The ethanol extract had significant cytotoxicity to HeLa cells and the bioactive fraction from the crude extract had antiproliferative effects with an IC50 value of<10µg/ml. Fluorescence microscopy, caspase and LDH activity assays were confirming the anticancer potential of fraction 6. This fraction had arrested the HeLa cells in G2/M phase and decrease in the percentage of cells in S phase. By LC-MS analysis, it was found to have an m/z value of 701.8 indicating it to be a novel one.

Conclusions: Here mango seeds have shown promising potential as a novel source for the isolation of a bioactive compound with anticancer activity.

 

Keywords: Anti-cancer, Antiproliferative, Bioactive fraction, Cytotoxic, HeLa cells, Mango seeds

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How to Cite
Timsina, B., and V. K. Nadumane. “MANGO SEEDS: A POTENTIAL SOURCE FOR THE ISOLATION OF BIOACTIVE COMPOUNDS WITH ANTI-CANCER ACTIVITY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 13, May 2015, pp. 89-95, https://innovareacademics.in/journals/index.php/ijpps/article/view/4535.