IN VITRO ANTIPLATELET ACTIVITY OF AN ISOFLAVANONE ISOLATED FROM METHANOLIC EXTRACT OF EMBLICA OFFICINALIS FRUITS
Keywords:Polyphenolic compound, Emblica officinalis, in vitro platelet aggregation, quercetin, isoflavanone
Objective: In this study, we aimed to isolate a polyphenolic compound, from polyphenolic rich fraction from Emblica officinalis fruits and also study its effect on adenosine diphosphate (ADP) and collagen induced in vitro platelet aggregation.
Methods: The polyphenolic rich fraction was prepared by 80% methanolic extraction. The residue was extracted successively with hexane, benzene, ethyl acetate and n- butanol. Ethyl acetate residue was selected for column chromatography because of its high polyphenolic content. It was subjected to repeated column chromatography of series with different eluents of increasing polarity. A brown amorphous powder was obtained from ethyl acetate: methanol (7:3) fraction. This sample was subjected to UV- visible spectrum, IR spectrum, 1H NMR, 13C NMR and electrospray mass spectrum (ES-MS) studies for its structural elucidation.
Results: A compound, 5, 7, 4'- trihydroxy 3'- methoxy isoflavanone was identified from polyphenolic rich ethyl acetate: methanol (7:3) fraction seperated from the 80% methanolic extract of Embica officinalis fruits by repeated column chromatography. Yield of compound was 421.05 mg/kg. This compound exhibited antiplatelet activity well comparable with that of quercetin.
Conclusion: The present study proved that the isolated compound 5, 7, 4'- trihydroxy 3'- methoxy isoflavanone exerts a significant inhibitory activity on ADP and collagen induced in vitro platelet aggregation which can be considered as an effective remedy for alleviating complications of cardiovascular diseases.
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