IN VITRO ANTIPLATELET ACTIVITY OF AN ISOFLAVANONE ISOLATED FROM METHANOLIC EXTRACT OF EMBLICA OFFICINALIS FRUITS

Authors

  • ANILA L. Department of Biochemistry, NSS College, Nilamel, Kollam, Kerala, India 691535 https://orcid.org/0000-0001-8781-4581
  • M. S. HASHIM Institute of Life Science (HILS), Karyavattom, Thiruvananthapuram, Kerala, India 695581

DOI:

https://doi.org/10.22159/ijpps.2022v14i11.46212

Keywords:

Polyphenolic compound, Emblica officinalis, in vitro platelet aggregation, quercetin, isoflavanone

Abstract

Objective: In this study, we aimed to isolate a polyphenolic compound, from polyphenolic rich fraction from Emblica officinalis fruits and also study its effect on adenosine diphosphate (ADP) and collagen induced in vitro platelet aggregation.

Methods: The polyphenolic rich fraction was prepared by 80% methanolic extraction.  The residue was extracted successively with hexane, benzene, ethyl acetate and n- butanol. Ethyl acetate residue was selected for column chromatography because of its high polyphenolic content. It was subjected to repeated column chromatography of series with different eluents of increasing polarity. A brown amorphous powder was obtained from ethyl acetate: methanol (7:3) fraction. This sample was subjected to UV- visible spectrum, IR spectrum, 1H NMR, 13C NMR and electrospray mass spectrum (ES-MS) studies for its structural elucidation.

Results: A compound, 5, 7, 4'- trihydroxy 3'- methoxy isoflavanone was identified from polyphenolic rich ethyl acetate: methanol (7:3) fraction seperated from the 80% methanolic extract of Embica officinalis fruits by repeated column chromatography. Yield of compound was 421.05 mg/kg. This compound exhibited antiplatelet activity well comparable with that of quercetin.

Conclusion: The present study proved that the isolated compound 5, 7, 4'- trihydroxy 3'- methoxy isoflavanone exerts a significant inhibitory activity on ADP and collagen induced in vitro platelet aggregation which can be considered as an effective remedy for alleviating complications of cardiovascular diseases.

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Published

20-09-2022

How to Cite

L., A., and M. S. HASHIM. “IN VITRO ANTIPLATELET ACTIVITY OF AN ISOFLAVANONE ISOLATED FROM METHANOLIC EXTRACT OF EMBLICA OFFICINALIS FRUITS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 14, no. 11, Sept. 2022, doi:10.22159/ijpps.2022v14i11.46212.

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