DUBIOUS ANTI-OBESITY AGENT HCA FROM GARCINIA: A SYSTEMATIC REVIEW


Ankur Gogoi, Nabajyoti Gogoi, Bijoy Neog

Abstract


Obesity is a complex disorder of appetite regulation and energy metabolism controlled by specific biological factors. Whenever prevention fails, medicinal treatment of obesity may become an obligation and it is more fruitful when we can acquire the medicinal treatment directly from nature, which is more preferred and healthier rather than going for chemical and surgical treatment. Alternatively, inhibition of carbohydrate to fatty acid conversion reaction can lead to obesity control. This can be done by assay of Hydroxycitric acid [(-)-HCA], which inhibits the formation of ATP-citrate lyase, responsible for lipogenesis. HCA is a derivative of citric acid and found in Garcinia fruit as the principal acid. Many in vitro and in vivo studies have demonstrated that (-)-HCA suppresses the de novo fatty acid synthesis and lipogenesis. However, results from clinical studies showed both negative and positive anti obesity effects of (-)-HCA. In this review paper an attempt has been made to explore and give an insight of (-)-HCA taking account of the literature coverage on speckled topics: Its discovery, properties, extraction and estimation and its significance of role in anti-obesity activity.

Keywords: Hydroxycitric acid, HCA, Garcinia, Anti-obesity, ATP-citrate lyase, Lipogenesis.

References


Kopelman PG. Obesity as a medical problem. Nature 2000;404(6778):635-43.

Bray GA, Tartaglia LA. Medicinal strategies in the treatment of obesity. Nature 2000;404:672-7.

Fogelholm M, Kukkonen-Harjula K, Oja P. Eating control and physical activity as determinants of short-term weight maintenance after a very-low-calorie diet among obese women. Int J Obes 1999;23:203-10.

Westerterp-Plantenga MS, Kempen KP, Saris WHM. Determinants of weight maintenance in women after diet-induced weight reduction. Int J Obes 1988;22:1-6.

CSIR: New Delhi, India. The Wealth of India (Raw Materials); 1956;4:99-108.

Hooker JD. The flora of British India; Vol. I. L. Reeve and Co. Ltd, Kent, England; 1874. p. 259-70.

Kanjilal UN, Kanjilal PC, Das A. Flora of Assam: Vol. I. Printed at Jay Offset Press: Delhi; 1940. p. 103-10.

Sreenivasan A, Venkataraman R. Chromatographic detection of the organic constituents of Gorikapuli (Garcinia cambogia Desr.) used in pickling fish. Curr Sci 1959;28:151-2.

Kuriyan KI, Pandya KC. A note on the main constituents of the dried rind of the fruit of Garcinia cambogia. J Indian Chem Soc 1931;8:469.

Lewis YS, Neelakantan S. (-)-Hydroxycitric acids-The principal acid in the fruits of Garcinia cambogia. Phytochemistry 1965;4:619-25.

Boll PM, Else S, Erik B. Naturally occurring lactones and lactames III. The absolute configuration of the hydroxycitric acid lactones, Hibiscus acid and Garcinia acid. Acta Chem Scand 1969;23:286-93.

Lewis YS. Isolation and properties of hydroxycitric acid. Methods Enzymol 1969;13:613-9.

Glusker JP, Minkin JA, Casciato CA. Absolute configuration of the naturally occurring hydroxycitric acids. Arch Biochem Biophys 1969;132:573-7.

Glusker JP, Minkin JA, Casciato CA. The structure and absolute configuration of the calcium salt of garcinia acid, the lactone of (-)-hydroxycitric acid. Acta Crystallogr 1971;B27:1284-93.

Stallings W, Blount TF, Srere PA. Structural studies of hydroxycitrate and their relevance to certain enzymatic mechanisms. Arch Biochem Biophys 1979;193:431-48.

Jayaprakasha GK, Sakariah KK. Determination of (-)-hydroxycitric acid in commercial samples of Garcinia cambogia extracts by liquid chromatography using ultraviolet detection. J Liq Chromatogr Relat Technol 2000;23:915-23.

Lowenstein JM. Effect of (-)-Hydroxycitrate on fatty acid synthesis by rat liver in vivo. J Biological Chem 1971;246(3):629-32.

Sullivan AC, Hamilton JG, Miller ON. Inhibition of lipogenesis in rat liver by (-)-hydroxycitrate. Arch Biochem Biophys 1972;150:183-90.

Sullivan AC, Triscari J, Hamilton JG. Effect of (-)-hydroxycitrate upon the accumulation of lipid in the rat. II. Appetite Lipids 1974;9:129-34.

Sullivan AC, Triscari J, Hamilton JG. Effect of (-)-hydroxycitrate upon the accumulation of lipid in rat I Lipogenesis. Lipids 1974;9:121-8.

Sullivan AC, Singh M, Sere PA. Reactivity and inhibitor potential of hydroxycitrate isomers with citrate synthase, citrate lyases and ATP citrate lyases. J Biol Chem 1977;252:7583-90.

Triscari J, Sullivan AC. Comparative effects of (-)-hydroxycitrate and (+)-allo-hydroxycitate on acetyl CoA carboxylase and fatty acid and cholesterol synthesis in vivo. Lipids 1977;12:357-63.

Sullivan AC. Effect of (-)-hydroxycitrate on lipid metabolism. In Modification of Lipid Metabolism; Academic Press: New York; 1984. p. 143-74.

Lowenstein JM, Bruneugraber H. Hydroxycitrate. Methods Enzymol 1981;72:486-97.

Moffett SA, Bhandari AK, Ravindranath B. Hydroxycitric acid concentrate and food products prepared therefrom US; 1996.

Moffett SA, Bhandari AK, Ravindranath B. Hydroxycitric acid concentrate and food products prepared therefrom US; 1997.

Jayaprakasha GK, Sakariah KK. Determination of organic acids in Garcinia cambogia (Desr.) by high-performance liquid chromatography. J Chromatogr A 1998;806:337-9.

Jayaprakasha GK, Sakariah KK. Determination of (-)-hydroxycitric acid in commercial samples of Garcinia cambogia extracts by liquid chromatography using ultraviolet detection. J Liq Chromatogr Relat Technol 2000;23:915-23.

Jayaprakasha GK, Sakariah KK. Determination of organic acids in leaves and rinds of Garcinia indica (Desr.) by LC. J Pharm Biomed Anal 2002;28:379-84.

Jena BS, Jayaprakasha GK, Sakariah KK. Organic acids from leaves, Fruits, and Rinds of Garcinia cowa. J Agric Food Chem 2002;50:3431-4.

Jayaprakasha GK, Jena BS, Sakariah KK. Improved liquid chromatographic method for determination of organic acids in Leaves, Pulp, Fruits, and Rinds of Garcinia. J AOAC Int 2003;86(5):1063-8.

Asish GR, Parthasarathy U, Zachariah TJ. A comparative estimation of (-) Hydroxycitric acid in different species of Garcinia. Hortic J 2008;21(1):26-9.

Muensritharam L, Tolieng V, Chaichantipyuth C. Capillary zone electrophoresis for separation and analysis of hydroxycitric acid and hydroxycitric acid lactone: Application to herbal products of Garcinia atroviridis Griff. J Pharm Biomed Anal 2008;46:577-82.

Vinh DO, Cuong DH, Thuong N. Extracting (-)-hydroxycitric acid from dried rinds of garcinia oblongifolia Champ. ex Benth by using microwave. J Korean Chem Soc 2011;55(6):983-7.

Naveen GPAN, Krishnakumar G. Atomic absorption spectroscopic analysis of fruit rind and seeds of Garcinia species. Indian J Sci 2012;1(1):61-3.

Naveen GPAN, Krishnakumar G. Biochemical analysis and seed oil Characterizations of Garcinia indica, G. xanthochymus and G. gummi-gutta for nutritional qualities. Indian J Sci 2012;1(1):71-3.

Naveen GPAN, Krishnakumar G. Traditional and Medicinal Uses of Garcinia gummi-gutta Fruit-A Review. Species 2013;4(10):4-5.

Kumar S, Sharma S, Chattopadhyay SK. Rapid and sensitive HPLC-PDA method for simultaneous identification and quantification of dietary weight reducing compound hydroxy citric acid lactone and chemo preventive compounds isoxanthochymol and xanthochymol in Garcinia indica. Int Food Res J 2013;20(1):397-402.

Upadhyay V, Tiwari A, Sharma N. Rapid determination & standardization of garcinia fruit extract of hydroxycitric acid (HCA) in Garcinia cambogia by HPLC. PHARMBIT 2013;27(1):25-31.

Gogoi A, Gogoi N, Neog B. Estimation of (-)-Hydroxycitric acid (HCA) in Garcinia Lanceaefolia Roxb. Using novel HPLC Methodology. Int J Pharm Sci Res 2014;5(11):4993-7.

Martius C, Maue R. Preparation, physiological behavior, and importance of hydroxycitric acid and its isomers. Z Physiol Chem 1941;269:33-9.

Majeed M, Badmaev V, Rajendran R. Potassium hydroxycitrate for the suppression of appetite and induction of weight loss US; 1998.

Balasubramanyam K, Chandrasekhar B, Ramadoss CS. Soluble double metal salt of group IA and IIA of (-)-hydroxycitric acid, process of preparing the same and its use in beverages and other food products without effecting their flavor and properties US; 2000.

Ibnusaud I, Puthiaparampil TT, Thomas B. Convenient method for the large-scale isolation of Garcinia acid US; 2000.

Watson JA, Fang M, Lowenstein JM. Tricarballylate and hydroxycitrate: Substrate and inhibition of ATP: citrate oxaloacetatae lyase. Arch Biochem Biophys 1969;135:209-17.

Watson JA, Lowenstein JM. Citrate and the conversion of carbohydrate into fat. J Biol Chem 1970;245:5993-6002.

Lowenstin JM. Citrate and the conversion of carbohydrate into fat. Biochem Soc Symp 1968;27:61-86.

Lehninger AL. Biochemistry. 2nd ed. Kalyani Publishers: New Delhi, India; 1983. p. 659-91.

Stryer L. Biochemistry. 3rd ed. Freeman: New York; 1988. p. 487-8.

Rawn JD. Biochemistry. Neil Patterson: Burlington, NC; 1997. p. 421-55.

Srere PA. The citrate cleavage enzyme. I. Distribution and purification. J Biol Chem 1959;234:2544-7.

Spencer A, Corman L, Lowenstein JM. Citrate and the conversion of carbohydrate into fat. Biochem J 1964;93:378-88.

D’Adamo AF Jr, Haft DE. An alternate of R-ketoglutarate catabolism in the isolated, perfused rat liver. J Biol Chem 1965;240:613-7.

Cheema-Dhadli S, Halperin M, Land Leznoff CC. Inhibition of enzymes which interact with citrate by (-)-hydroxycitrate and 1, 2, 3-tricarboxybenzene. Eur J Biochem 1973;38:98-102.

Szutowicz A, Stepien M, Lysiak W. Effect of (-)-hydroxycitrate on the activities of ATP citrate lyase and the enzymes of acetyl-CoA metabolism in rat brain. Acta Biochim Pol 1976;23:227-34.

Yamada T, Hida H, Yamada Y. Chemistry, physiological properties and microbial production of hydroxycitric acid. Appl Microbiol Biotechnol 2007;75(5):977-82.

Bray GA, Greenway FL. Pharmacological approaches to treating the obese patient. Clin Endocrinol Metab 1976;5:455-79.

Beynen AC, Geelen MJ. Effect of insulin and glucagon on fatty acid synthesis from acetate by hepatocytes incubated with (-)-hydroxycitrate. Endokrinologie 1982;79:308-10.

Barth C, Hackenschmidt J, Ullmann H. Inhibition of cholesterol synthesis by (-)-hydroxycitrate in perfused rat liver. Evidence for an extramitochondrial mevalonate synthesis from acetyl coenzyme A. FEBS Lett 1972;22:343-6.

Gregolin C, Ryder E, Warner RC. Liver acetyl coenzyme A carboxylase. II. Further molecular characterization. J Biol Chem 1968;243:4236-45.

Hashimoto T, Numa S. Kinetic studies on the reaction mechanism and the citrate activation of liver acetyl coenzyme A carboxylase. Eur J Biochem 1971;18:319-31.

Hackenschmidt J, Barth C, Decker K. Stimulation of acetyl-CoA carboxylase by (-)-hydroxycitrate. FEBS Lett 1972;27:131-3.

Hood RL, Beitz DC, Johnson DC. Inhibition by potential metabolic inhibitors of in vitro adipose tissue lipogenesis. Comp Biochem Physiol 1985;81(3):667-70.

Rao RN, Sakariah KK. Lipid-lowering and antiobesity effect of (-)-hydroxycitric acid. Nutr Res 1988;8:209-12.

Vicario C, Medina M. Metabolism of lactate in the rat brain during the early neonatal period. J Neurochem 1992;59:32-40.

Clouatre D, Rosenbaum M. The diet and health benefits of HCA (Hydroxycitric Acid). Keats Publishing: New Cannan CT; 1994.

Heymsfield SB, Allison DB, Vasselli JR. Garcinia cambogia (hydroxycitric acid) as a potential antiobesity agent: A randomized controlled trial. J Am Med Assoc 1998;280(18):1596-600.

Kriketos AD, Thompson HR, Greene H. (-)-hydroxycitric acid does not affect energy expenditure and substrate oxidation in adult males in a post-absorptive state. Int J Obes 1999;23:867-73.

Mattes RD, Bormann L. Effects of (-)-hydroxycitric acid on appetitive variables. Physiol Behav 2000;71:87-94.

Mahendran P, Devi CSS. The modulating effect of Garcinia cambogia extract on ethanol induced peroxidation. Indian J Pharmacol 2001;33(2):87-91.

Leonhardt M, Hrupka B, Langhans W. Effect of hydroxycitrate on food intake and body weight regain after a period of restrictive feeding in male rats. Physiol Behav 2001;74:191-6.

Kovacs EMR, Westerterp-Plantenga MS, Saris WHM. The effects of 2-week ingestion of (-)-hydroxycitrate and (-)-hydroxycitrate combined with medium-chain triglycerides on satiety, fat oxidation, energy expenditure and body weight. Int J Obes 2001;25:1087-94.

Westerterp-Plantenga MS, Kovacs EMR. The effect of (-)-hydroxycitrate on energy intake and satiety in overweight humans. Int J Obes 2002;26(6):870-2.

Shara M, Ohia SE, Yasmin T. Dose-and time-dependent effects of a novel (-)-hydroxycitric acid extract on body weight, hepatic and testicular lipid peroxidation, DNA fragmentation and histopathological data over a period of 90 days. Mol Cell Biochem 2003;254:339-46.

Hayamizu K, Hirakawa H, Oikawa D. Effect of Garcinia cambogia extract on serum leptin and insulin in mice. Fitoterapia 2003;74:267-73.

Hayamizu K, Ishii Y, Shigematsu N. Safety of Garcinia cambogia extract in healthy men: high dose administration study I. J Oleo Sci 2003;52(9):499-504.

Hayamizu K, Ishii Y, Kaneko I. Effects of Garcinia cambogia (Hydroxycitric Acid) on visceral fat accumulation: A Double-Blind, Randomized, Placebo-Controlled Trial. Curr Ther Res 2003;64(8):551-67.

Hayamizu K, Tomi H, Kaneko I. Effects of Garcinia cambogia extract on serum sex hormones in overweight subjects. Fitoterapia 2008;79:255-61.

Preuss HG, Bagchi D, Bagchi M. Efficacy of a novel, natural extract of (-)-hydroxycitric acid (HCA-SX) and a combination of HCA-SX, niacinbound chromium and Gymnema sylvestre extract in weight management in human volunteers: a pilot study. Nutr Res 2004;24:45-58.

Preuss HG, Bagchi D, Bagchi M. Effects of a natural extract of (-)-hydroxycitric acid (HCA-SX) and a combination of HCA-SX plus niacin-bound chromium and Gymnema sylvestre extract on weight loss. Diabetes Obes Metab 2004;6:171-80.

Soni MG, Burdock GA, Preuss HG. Safety assessment of (-)-hydroxycitric acid and Super CitriMax!, a novel calcium/potassium salt. Food Chem Toxicol 2004;42:1513–29.

Saito M, Ueno M, Ogino S. High dose of Garcinia cambogia is effective in suppressing fat accumulation in developing male Zucker obese rats, but highly toxic to the testis. Food Chem Toxicol 2005;43:411-9.

Burdock G, Soni M, Bagchi M. "Garcinia cambogia toxicity is misleading". Food Chem Toxicol 2005;43(11):1683-4.

Downs BW, Bagchi M, Subbaraju GV. Bioefficacy of a novel calcium-potassium salt of (-)-hydroxycitric acid. Mutat Res 2005;579:149-62.

Deshmukh NS, Bagchi M, Yasmin T. Safety of a novel calcium/potassium salt of (-)-Hydroxycitric acid (HCA-SX): II. Developmental toxicity study in rats. Toxicol Mech Methods 2008;18:443-51.

Brandt K, Langhans W, Geary N. Beneficial and deleterious effects of hydroxycitrate in rats fed a high-fructose diet. Nutrition 2006;22:905-12.

Ates A, Gursel FE, Altıner A. Effect of Garcinia cambogia extract on fatty liver in rats fed high lipid. Kafkas Universitesi Veteriner Fakultesi Dergisi 2011;17(6):1015-20.

Ates A, Gursel FE, Bilal T. Effect of dietary Garcinia cambogia extract on serum lipid profile and serum enzymes in rats fed high-lipid diet. Iranian J Vet Res 2012;13(1):1-7.

Onakpoya I, Hung SK, Perry R. The use of garcinia extract (Hydroxycitric Acid) as a weight loss supplement: a systematic review and meta-analysis of randomised clinical trials. J Obes 2011. doi: 10.1155/2011/509038 [Article in Press].

Kim J, Jeon S, Park KH. Does glycine max leaves or Garcinia Cambogia promote weight-loss or lower plasma cholesterol in overweight individuals: a randomized control Trial. Nutr J 2011;10:94.

Kim J, Kyung J, Kim D. Anti-obesity effects of Rapha diet® preparation in mice fed a high-fat diet. Lab Anim Res 2012;28(4):265-71.

Bilal T, Gursel FE, Ates A. Effect of Garcinia cambogia extract on body weight gain, feed intake and feed conversion ratio, and serum non-esterified fatty acids and C-reactive protein levels in rats fed with atherogenic diet. Iranian J Vet Res 2012;13(4):330-3.

Altiner A, Ates A, Gursel E. Effect of the antiobesity agent Garcinia cambogia extract on serum lipoprotein (a), Apolipoproteins A1 and B, and total Cholesterol levels in female rats fed atherogenic diet. J Anim Plant Sci 2012;22(4):872-7.

Chuah LO, Yeap SK, Ho WY. “In vitro and in vivo toxicity of Garcinia or hydroxycitric acid: a review,”. Evidence-Based Complementary Altern Med 2012. doi: 10.1155/2012/197920. [Article in Press].

Chuah LO, Ho WY, Beh BK. Updates on antiobesity effect of Garcinia Origin (-)-HCA. Evidence-Based Complementary and Alternative Medicine 2013. doi: 10.1155/2013/751658 [Article in Press].

Kim YJ, Choi MS, Park YB. Garcinia Cambogia attenuates diet-induced adiposity but exacerbates hepatic collagen accumulation and inflammation. World J Gastroenterol 2013;19(29):4689-701.

Clouatre DL, Preuss HG. Hydroxycitric acid does not promote inflammation or liver Toxicity. World J Gastroenterol 2013;19(44):8160-2.

Sethi A, Patidar D, Patidar N. Formulation of sustained release tablet of antiobesity drug Garcinia cambogia. Int J Pharm Res Dev 2012;3(12):56-63.

Patel R, Patel J, Kakkar S. Formulation and development of anti-obesity liquid formulation containing garcinia cambogia extract, l-carnitine & chromium picolinate. Indo Global J Pharm Sci 2013;3(1):40-51.



About this article

Title

DUBIOUS ANTI-OBESITY AGENT HCA FROM GARCINIA: A SYSTEMATIC REVIEW

Date

08-05-2015

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Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 7, Issue 7, 2015 Page: 1-8

Online ISSN

0975-1491

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Authors & Affiliations

Ankur Gogoi
Centre for Studies in Biotechnology, Dibrugarh University, Assam, India
India

Nabajyoti Gogoi
Centre for Studies in Biotechnology, Dibrugarh University, Assam, India
India

Bijoy Neog
Department of Life Sciences, Dibrugarh University, Assam, India
India

 

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