• Radhakrishnan Gowri Department of Pharmacognosy, Faculty of Pharmacy, M. S. Ramaiah University of Applied Sciences, Bangalore, Karnataka 560054
  • Varadharajan Madhavan Department of Pharmacognosy, Faculty of Pharmacy, M. S. Ramaiah University of Applied Sciences, Bangalore, Karnataka 560054
  • Sunkam Yoganarasimhan Department of Pharmacognosy, Faculty of Pharmacy, M. S. Ramaiah University of Applied Sciences, Bangalore, Karnataka 560054


Objective: To evaluate the protective effect of ethanol extract of whole plant of Sphaeranthus amaranthoides against paracetamol induced hepatotoxicity in albino wistar rats.

Methods: Liver damage was induced by administration of paracetamol orally at a dose of 2 g/kg body weight. Protective effect of the extract was determined by assessing the levels of serum enzymes like AST, ALT, ALP, total protein, total bilirubin, liver Malondialdehyde and glutathione levels. The extract was standardized by HPTLC method.

Results: Administration of ethanol extract at 400 mg/kg b. wt showed significant (***P<0.001) reduction in elevated serum enzyme levels compared to paracetamol induced animals. Paracetamol induced liver toxicity decreased the liver glutathione level and increased the MDA level. The results of our study showed that ethanol extract at 400 mg/kg significantly increased glutathione content and decreased the MDA level indicating the protective role of Sphaeranthus amaranthoides ethanol extract against paracetamol induced liver toxicity.

Conclusion: Sphaeranthus amaranthoides whole plant possesses potent hepatoprotective activity against paracetamol induced hepatotoxicity.


Keywords: Sphaeranthus amaranthoides, Hepatoprotective, Paracetamol, Biochemical parameters, Lipid peroxidation


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Author Biography

Radhakrishnan Gowri, Department of Pharmacognosy, Faculty of Pharmacy, M. S. Ramaiah University of Applied Sciences, Bangalore, Karnataka 560054

Assistant Professor,Department of pharmacognosy,
Faculty of pharmacy,
M.S.Ramaiah University of Applied Sciences


1. Linda BS, Myron JT, Ronald WB, Jonathan RH. Acetaminophen hepatotoxicity and acute liver failure. J Clin Gastroenterol 2009;43:342–9.
2. Visen PK, Shukla B, Patnaik GK, Dhawan BN. Andrographolide protect rat hepatocytes against paracetamol–induced damage. J Ethnopharmacol 1993;40:131-6.
3. Panchabai TS, Ambarkhane SV, Joshi AS, Samant BD, Rege NN. Protective effect of Tinospora cordifolia, Phyllanthus emblica and their combination against antitubercular drugs induced hepatic damage: An experimental study. Phytother Res 2008;22:646-50.
4. Das SK, Vasudevan DM. Protective effects of Silymarin, a milk thistle (Silybium marianum) derivative on ethanol induced oxidative stress in liver. Indian J Biochem Biophys 2006;43:306-11.
5. Yoganarasimhan SN. Medicinal plants of India (Tamilnadu). Vol II. Bangalore: Interline publishing private Ltd; 2000. p. 512.
6. Anonymous. The Ayurvedic Pharmacopoeia of India. Ministry of Health and Family Welfare, Government of India, New Delhi; 2001. p. 127-8.
7. Anonymous. The Ayurvedic Formulary of India. Ministry of Health and Family planning, Government of India, New Delhi; 1978. p. 209.
8. Sharma PC, Yelne MB, Dennis TJ. Database on medicinal plants used in Ayurveda-V-5. New Delhi: Central council for Research in Ayurveda and Siddha, Government of India; 2002. p. 251-9.
9. Geethalakshmi R, Sakravarthi C, Kritika T, Arul Kirubakaran M, Sarada DV. Evaluation of antioxidant and wound healing activity of Sphaeranthus amaranthoides Burm. f. BioMed Res Int 2013. doi.org/10.1155/2013/607109. [Article in press]
10. Swarnalatha L, Neelakanta Reddy P. Hepatoprotective activity of Sphaeranthus amaranthoides on D-galactosamine induced hepatitis in albino rats. Asian J Trop Biomed 2012;2(3):S1900–5.
11. Gamble JS. Flora of the presidency of madras. Vol. II. Dehra Dun: Bishen Singh Mahendra Pal Sing; 1921. p. 691.
12. Kokate CK. Practical Pharmacognosy. New Delhi: Vallabh Prakashan; 1999. p. 107-21.
13. Harborne JB. Phytochemical Methods. A guide to modern techniques of plant analysis, 3rd ed. London: Chapman and Hall; 1998. p. 4-7.
14. Raaman N. Phytochemical Techniques. New Delhi: New India Publishing Agency; 2006. p. 19-24.
15. Brain KR, Turner TD. The Practical evaluation of Phyto pharmaceuticals. Bristol: Wright–Scientechnica; 1975. p. 82.
16. Wagner H, Bladt S. Plant drug analysis. 2nd ed. Bristol: Springer; 1996. p. 54-254.
17. OECD/OCDE. OECD guidelines for testing of Chemical-423; 2001.
18. Kulkarni SK. Hand book of Experimental Pharmacology. New Delhi: Vallabh Prakashan; 1999. p. 168-71.
19. Nirmala M, Girija K, Lakshman K, Divya T. Hepatoprotective activity of Musa paradisica on experimental animal models. Asian Pac J Trop Biomed 2012;2(1):11-5.
20. Jose MM, Aledo JL, Cristina PG, Estebandelvalle MS. Interrelationship between oxidative damage and antioxidant enzyme activities: an easy and rapid experimental approach. Biochem Educ 2000;28(2):93-5.
21. Chatterjee M, Sil PC. Hepatoprotective effect of aqueous extract of Phyllanthus niruri on nimesulide induced oxidative stress in vivo. Indian J Biochem Biophys 2006;43:299-305.
22. Sumanth M, Ahmed R. Antihepatotoxic and antioxidant activity of root of Taraxacum officinale in CCl4-intoxicated rats. Phcog Mag 2008;16(4):188–94.
23. Koop DR. Oxidative and reductive metabolism by cytochrome P450 2E1. FASEB J 1992;6:724–30.
24. Lee WM. Acetaminophen and the U. S. Acute Liver Failure Study Group: lowering the risks of hepatic failure. Hepatology 2004;40:6-9.
25. Larson AM, Polson J, Fontana RJ. Acetaminophen induced acute liver failure: results of a United States multicenter, prospective study. Hepatology 2005;42:1364-72.
26. Bernal W. Changing patterns of causation and the use of transplantation in the United Kingdom. Semin Liver Dis 2003;23:227–37.
27. Nanji AA, Jokelainen K, Foutouhinia M, Rahemtulla A, Thomas P, Tipoe GL. Increased severity of alcoholic liver injury in female rats: role of oxidative stress, endotoxin and chemokines. Am J Physiol: Gastrointest Liver Physiol 2002;281:G1348–56.
28. Kapur V, Pillai KK, Hussain SZ, Balani DK. Hepatoprotective activity of Jigrine on liver damage caused by alcohol-carbon tetrachloride and Paracetamol in rats. Indian J Pharmacol 1994;26:35-40.
29. Jollow DJ, Thorgeirsson SS, Potter WZ, Hashimoto M, Mitchell JR. Acetaminophen induced hepatic necrosis. VI. Metabolic disposition of toxic and nontoxic doses of acetaminophen. Pharmacology 1974;12:251–71.
30. Taniguchi H, Yomota E, Nogi K, Onoda Y. Effects of anti-ulcer agents on ethanol-induced gastric mucosal lesions in D-Gal N induced hepatitis rats. Drug Res 2004;2:600-4.
31. Keppler D, Pausch J, Decker K. Selective uridine triphosphate deficiency induced by D-galactosamine in liver and reversed by pyrimidine nucleotide precursors. Effect on ribonucleic acid synthesis. J Biol Chem 1974;249:211-6.
32. Quintero A, Pedraja CA, Siendones E, Kamal EI, Said AM, Colell A, et al. PGE1 protection against apoptosis induced by D-galactosamine is not related to the modulation of intracellular free radical production in primary culture of rat hepatocytes. Free Radical Res 2002;36:345-55.
33. Sire O, Mangenev M, Montagne J, Nordmann R, Nordmann J. Carntine palmitoyltransferase I. Inhibition by D-galactosamine and role of phospholipids. Eur J Biochem 1983;136:371-5.
34. Juraj G, Zuzana C, Maria M, Zenobia C, Vladimira T, Gabriela M, et al. Effect of quercetin on paracetamol-induced rat liver mitochondria dysfunction. Biologia Bratislava 2004;59 (3):399-403.
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How to Cite
Gowri, R., V. Madhavan, and S. Yoganarasimhan. “HEPATOPROTECTIVE ACTIVITY OF SPHAERANTHUS AMARANTHOIDES WHOLE PLANT IN PARACETAMOL INDUCED HEPATOTOXICITY IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 8, June 2015, pp. 82-86, https://innovareacademics.in/journals/index.php/ijpps/article/view/5516.
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