PHARMACOGNOSTICAL AND ANTIMICROBIAL STUDIES OF THE STEM OF TABERNAEMONTANA DIVARICATA LINN
Objective: The aim of the present study is to evaluate the pharmacognostic and antimicrobial studies of Tabernaemontana divaricata Linn. Stem.
Methods: The Pharmacognostical studies were performed using standard parameters and antimicrobial studies were based on checker board and disc diffusion technique using various bacterial strains. The antifungal studies were based on the agar slant technique.
Results: The transverse section shows the presence of undifferentiated cells known as cortex in the outer region. They are followed by an extended part of cortex, which consists of 7-8 layers of Phellogen, followed by 6-7 layers of Phelloderm. The antimicrobial studies confirmed that the methanolic extract was quite effective for bacterial strains Sh. flexneri type BCH 995, Shigella boydii 8, Sh. sonnei NK 840, Sh. dysenteriae 1, Sh. dysenteriae 9, Vibrio cholerae 1023, V. cholerae 1341, V. cholerae 575, V. cholerae 1311, E. coli RH 07/12, E. coli 18/9, E. coli K88, Enterobacter spp AP596, Staphylococcus aureus ML 267, S. aureus MTCC 96, Bacillus subtilis MTCC 441, Pseudomonas auriginosa AP585 NLF, Bacillus pumilus 8241 and Klebsiella pneumoniae. The maximum antibacterial activity was observed against Staphylococcus aureus MTCC 96 (zone of inhibition at 800Âµg/ml is 12.17Â±0.124 mm), whereas minimum activity against E. coli RH 07/12 (zone of inhibition at 800Âµg/ml is 10.45Â±0.063 mm). It was also effective against the fungal strains Aspergillus niger MTCC 281, Candida albicans ATCC 10231 and Penicillium chrysogenum MTCC 2725.
Conclusion: The study of pharmacognostical features of the stem of T. divaricata Linn. May serves as a tool for identification and standardization of the crude drug as per WHO guidelines.
2. Prajapati ND, Purohit SS, Sharma AK, Kumar T. Handbook of Medicinal Plants. 2nd ed. India: Agrobios Publishers; 2004.
3. Anonymous. The wealth of india-a dictionary of indian raw materials and industrial products. 2nd ed. New Delhi (India): CSIR Publication; 2003;3:192-3.
4. Nadkarni KM. Indian Materia Medica. 3rd ed. Bombay (India): Popular Prakashan; 1976. p. 1189-90.
5. Van Beek TA, Verpoorte R, Svendsen AB, Leeuwenberg AJ, Bisset NG. Tabernaemontana L. (Apocynaceae): a review of its taxonomy, phytochemistry, ethnobotany and pharmacology. J Ethnopharmacol 1984;10:1-156.
6. Okonogi S, Neimkhum W, Niwatananun W, Yotsawimonwat S. Biological activities of active extracts from stem of Tabernaemontana divaricata. J Met Mater Miner 2010;20(3):201-6.
7. Khan MSA. Gastroprotective Effect of Tabernaemontana divaricata (Linn.) R. Br. flower methanolic extract in wistar rats. Br J Pharm Res 2011;1(3):88-98.
8. Sharker SM, Chakma S, Rahman AA. Phytochemical and antinociceptive study of leaves of Tabernaemontana divaricata (L). J Med Plants Res 2011;5(2):245-7.
9. Pratchayasakul W, Pongchaidecha A, Chattipakorn N, Chattipakorn SC. Reversible acetylcholinesterase inhibitory effect of Tabernaemontana divaricata extract on synaptic transmission in rat CA1 hippocampus. Indian J Med Res 2010;131:411-7.
10. Khandelwal KR. Practical Pharmacognosy-Techniques and Experiments. 22nd ed. Pune (India): Nirali Prakashan; 2012:23:6-25, 6.
11. Kokate CK. Practical Pharmacognosy. 4th ed. New Delhi (India): Vallabh Prakashan; 2008. p. 107-24.
12. Mazumder R, Mendiratta T, Mandal SC, Mazumder A. Antimicrobial potency of the leaf stalk extract of Curcuma longa Roxb. Anc Sci Life 2000;20:92-6.
13. Mazumder A, Singh SK, Mazumder R, Basu SP, Saha BP. Antimicrobial action of the leaf extract of Lagerstroemia parviflora Roxb. Anc Sci Life 2001;21(3):198-201.