EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF STEM EXTRACTS OF CUSCUTA REFLEXA (ROXB) IN RATS
Keywords:Cuscuta reflexa, Stem extracts, Paracetamol, Silymarin, Hepatoprotective activity
Objective: Cuscuta Reflexa (Convolvulaceae) is a plant with a variety of ethnic medicinal uses along with antioxidant activity. Hence it was planned to evaluate the hepatoprotective activity with alcoholic extracts of stem of Cuscuta reflexa (AESCR) and aqueous extracts of stem of Cuscuta reflexa (AQESCR).
Methods: Hepatoprotective activity of both the extracts was studied against paracetamol induced hepatotoxicity in rats. Functional (thiopentone induced sleeping time), physical (wet liver weight and volume), biochemical parameters Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Serum alkaline phosphatase (ALP), Serum direct bilirubin (BILD), Serum total bilirubin (BILT), Serum albumin (ALB), Serum total proteins (PRO), Serum cholesterol (CHO), and histopathological changes of livers were assessed in control/toxicant/standard/and extract treated animals with paracetamol induced hepatotoxic models in rats.
Results: In LD50 studies for AESCR and AQESCR up to the maximum dose level of 2000 mg/kg dose no mortality was observed in any of the animals, indicating the practically nontoxic. When compared to toxicant control groups both the extracts have significantly reduced the paracetamol induced elevated levels of serum ALT, AST, ALP, BILT, BILD, CHO, and elevated the levels of ALB and PRO. The histopathological changes (steatosis), necrosis etc. Were partly or fully prevented in animals treated with the two extracts.
Conclusion: AESCR and AQESCR showed a significant hepatoprotective effect against paracetamol induced hepatic damage. The medium and high doses of AESCR and AQESCR (200 and 400 mg/kg) treated groups showd better hepatoprotective activity when compared to standard drug silymarin (25 mg/kg).
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