KINETIC SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF GENTAMICIN IN PHARMACEUTICAL FORMS
Keywords:Gentamicin, Spectrophotometric, Determination, Oxidation, Drug formulation
Objective: The objective of this study is to develop and validate new selective kinetic methods for the determination of Gentamicin in pure and pharmaceutical preparations.
Methods: The proposed methods (A and B) are based on the oxidation of Gentamicin drug with alkaline potassium permanganate at fixed temperature. The oxidation reaction was followed spectrophotometrically by measuring the increase in the absorbance owing to the formation of MnO42-at 610 nm. These methods (A) and (B) are based on initial-slope and fixed-time methods respectively.
Results: The methods were linear in the concentration ranges 20â€“125 and 25-140 Î¼g/ml with correlation coefficients of 0.995 and 0.988 and limits of detection LOD of 10.5 and 9.2 Âµg/ml for the two methods respectively. The proposed methods were applied for the determination of gentamicin in pharmaceutical formulations with recovery and relative standard deviations of 97%Â±8 and 98%Â±7 for the two methods.
Conclusion: the developed methods were accurate, precise and reproducible compared to the official methods.
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