EVALUATION OF PUNICA GRANATUM FRUIT PEELS EXTRACTS FOR ITS FREE RADICAL SCAVENGING AND ANTI-INFLAMMATORY ACTIVITY

  • Deepali C. Mahajan Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Women’s University, Santacruz (W), Mumbai 400049, India
  • Usha S. Satyapal Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Women’s University, Santacruz (W), Mumbai 400049, India
  • Pratima A. Tatke Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Women’s University, Santacruz (W), Mumbai 400049, India
  • Vikram A. Naharwar Amsar Goa Pvt. Ltd., Colvale, Goa 403513, India.

Abstract

Objective: To evaluate free radical scavenging and anti-inflammatory activity of petroleum ether, ethyl acetate, aqueous, methanol:water and methanol extracts of Punica granatum fruit peels (PGFP) (Family: Lythraceae) by in vitro methods.

Methods: The free radical scavenging effect was studied using 1,1â€Diphenylâ€2â€Picrylhydrazyl (DPPH) and nitric oxide radical scavenging assay. Antiâ€inflammatory activity was evaluated by HRBC membrane stabilization assay.

Results: All the extracts of PGFP exhibited significant free radical scavenging effect. The methanol extract exhibited maximum significant DPPH and nitric oxide radical scavenging activity with IC50 value of 24.43 and 45.56µg/ml and maximum stabilization (86.96%) of HRBC membrane at 80 µg/ml among all the extracts of PGFP.

Conclusion: Methanol as an extraction solvent was found to be the best in obtaining the extract of PGFP rich in radical scavenging and anti-inflammatory phytoconsituents.

 

Keywords: Punica granatum fruit peel, DPPH assay, NO assay, HRBC membrane, Antioxidant, Anti-inflammatory

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Author Biographies

Deepali C. Mahajan, Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Women’s University, Santacruz (W), Mumbai 400049, India
Senior research Fellow
Usha S. Satyapal, Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Women’s University, Santacruz (W), Mumbai 400049, India
Senior research Fellow
Pratima A. Tatke, Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Women’s University, Santacruz (W), Mumbai 400049, India

Department of Pharmaceutical Chemistry,

Professor of Pharmaceutical Chemistry

Vikram A. Naharwar, Amsar Goa Pvt. Ltd., Colvale, Goa 403513, India.
Director, Amsar Goa Pvt Ltd

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How to Cite
Mahajan, D. C., U. S. Satyapal, P. A. Tatke, and V. A. Naharwar. “EVALUATION OF PUNICA GRANATUM FRUIT PEELS EXTRACTS FOR ITS FREE RADICAL SCAVENGING AND ANTI-INFLAMMATORY ACTIVITY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 7, May 2015, pp. 222-5, https://innovareacademics.in/journals/index.php/ijpps/article/view/6104.
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