IN VITRO ASSESSSMENT OF ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS FROM LICHEN RAMALINA NERVULOSA

  • Jeya Prakash Sundararaj Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India
  • Selvambigai Kuppuraj Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India
  • Ayyappadasan Ganesan Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India
  • Ponmurugan Ponnusamy Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India
  • Sanjeeva Nayaka Lichenology Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001

Abstract

Objective: The purpose of the existent study is to assess the in vitro antioxidant and antimicrobial activity of different solvent extracts of Ramalina nervulosa.

Methods: Folin ciocalteu’s and Aluminium chloride method were used for total phenolic and flavonoid content estimation respectively. The antioxidant activity was evaluated through free radical scavenging activity by DPPH (2, 2-diphenyl-1-picrylhydrazyl), FRAP (ferric ions reducing antioxidant potential), cupric ion chelation ability. Agar diffusion method was used to assess the antimicrobial capacity. GC-MS analysis was carried out to identify the presence of compounds in the extracts.

Results: Ethanol extracts exhibited that the highest zone of inhibition with Bacillus subtilis, Proteus vulgaris, Pseudomonas aeruginosa, Klebsiella pneumoniae and with two fungal strains such as Fusarium oxyparum and Penicillium notatum. The extract also has high inhibition capacity of 64.29% and 62.49% towards DPPH and Cu2+ion respectively. But the aqueous extract showed higher amount of phenolic and flavonoid content of about 211.40 mg GAE/g dw and 50.72 mg QE/g dw respectively and Fe2+ ion reducing capacity of about 144.41 mol ascorbic acid equivalent/g db. Further analysis of GC-MS showed the presence of various bioactive molecules present in the lichen Ramalina nervulosa.

Conclusion: The results of FRAP assay were positively correlated with the total phenolic and flavonoid content. Ethanol extracts of R. nervulosa showed promising antibacterial, antifungal and antioxidant activities which can be considered as the potent source of antioxidant product and offer protection from oxidative stress under many pathophysiological conditions.

 

Keywords: DPPH, FRAP, Antioxidant, GC-MS, Free radical scavenging activity

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Author Biographies

Jeya Prakash Sundararaj, Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India

The Department of Biotechnology, K.S.Rangasamy College of Technology is 4th ranked among the 280 private colleges offering Biotechnology programme as per "Biospectrum" survey.

The department has equiped with 11 funded projects from different funding agencies such as ICMR, DBT, DST, UGC DRDO etc.,

The department has totally 15 laboratories such as Biochemistry, Bioorganic Chemistry, Bioprocess, Plant Tissue Culture, Animal cell line, Molecular Biology/Genetic Engineering, Bioinformatics, Immunology, Cell Biology, Microbiology, Downstream processing, Cyanobacteria lab and Instrumentation. 

Around 16 students and 5 faculty memebers have received ‘Summer Research Fellowship’ from Indian Academy of Sciences (IASc), Bangalore. So far, 

21 ‘Student Project Scheme’ were received from Tamil Nadu State council of Science and Technology (TNSCST), Chennai for which a total outlay of Rs.2,80,000/- was sanctioned. A total of seven staff members were received ‘Young Scientist Award’ and ‘Summer Faculty Research Fellowship from TNSCST, Chennai and Indian Institute of Technology, New Delhi, respectively for which the recipients were carried out a project work for a period of two months at reputed institutions such as IITs, IISc, IASc, University departments and other R&D centers. 

Around 16 text books were published by the department faculty members in which two text books entitled “Hand Book of Biotechnology and Chemical Engineering†(ISBN: 978-81-224-3144-5) and “Applications of Plant Tissue Culture†(ISBN: 978-81-224-3144-6) have been published by “New Age International, New Delhi on 25th July 2010 and etc. 

In addition, a total of 155 research papers were published in National Journals and 87 research papers were published in International Journals and nearly 240 abstracts papers were published at different National and International conferences / symposia by the students and staff members. A total of five granted patents and 15 patents were filed very recently subsequently another three patents are under pipeline at national and international level especially in US and UK. Eleven DNA sequences of plant pathogenic strains were deposited at NCBI, Maryland, USA.

Selvambigai Kuppuraj, Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India

The Department of Biotechnology, K.S.Rangasamy College of Technology is 4th ranked among the 280 private colleges offering Biotechnology programme as per "Biospectrum" survey.

The department has equiped with 11 funded projects from different funding agencies such as ICMR, DBT, DST, UGC DRDO etc.,

The department has totally 15 laboratories such as Biochemistry, Bioorganic Chemistry, Bioprocess, Plant Tissue Culture, Animal cell line, Molecular Biology/Genetic Engineering, Bioinformatics, Immunology, Cell Biology, Microbiology, Downstream processing, Cyanobacteria lab and Instrumentation. 

Around 16 students and 5 faculty memebers have received ‘Summer Research Fellowship’ from Indian Academy of Sciences (IASc), Bangalore. So far, 

21 ‘Student Project Scheme’ were received from Tamil Nadu State council of Science and Technology (TNSCST), Chennai for which a total outlay of Rs.2,80,000/- was sanctioned. A total of seven staff members were received ‘Young Scientist Award’ and ‘Summer Faculty Research Fellowship from TNSCST, Chennai and Indian Institute of Technology, New Delhi, respectively for which the recipients were carried out a project work for a period of two months at reputed institutions such as IITs, IISc, IASc, University departments and other R&D centers. 

Around 16 text books were published by the department faculty members in which two text books entitled “Hand Book of Biotechnology and Chemical Engineering†(ISBN: 978-81-224-3144-5) and “Applications of Plant Tissue Culture†(ISBN: 978-81-224-3144-6) have been published by “New Age International, New Delhi on 25th July 2010 and etc. 

In addition, a total of 155 research papers were published in National Journals and 87 research papers were published in International Journals and nearly 240 abstracts papers were published at different National and International conferences / symposia by the students and staff members. A total of five granted patents and 15 patents were filed very recently subsequently another three patents are under pipeline at national and international level especially in US and UK. Eleven DNA sequences of plant pathogenic strains were deposited at NCBI, Maryland, USA.

Ayyappadasan Ganesan, Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India

The Department of Biotechnology, K.S.Rangasamy College of Technology is 4th ranked among the 280 private colleges offering Biotechnology programme as per "Biospectrum" survey.

The department has equiped with 11 funded projects from different funding agencies such as ICMR, DBT, DST, UGC DRDO etc.,

The department has totally 15 laboratories such as Biochemistry, Bioorganic Chemistry, Bioprocess, Plant Tissue Culture, Animal cell line, Molecular Biology/Genetic Engineering, Bioinformatics, Immunology, Cell Biology, Microbiology, Downstream processing, Cyanobacteria lab and Instrumentation. 

Around 16 students and 5 faculty memebers have received ‘Summer Research Fellowship’ from Indian Academy of Sciences (IASc), Bangalore. So far, 

21 ‘Student Project Scheme’ were received from Tamil Nadu State council of Science and Technology (TNSCST), Chennai for which a total outlay of Rs.2,80,000/- was sanctioned. A total of seven staff members were received ‘Young Scientist Award’ and ‘Summer Faculty Research Fellowship from TNSCST, Chennai and Indian Institute of Technology, New Delhi, respectively for which the recipients were carried out a project work for a period of two months at reputed institutions such as IITs, IISc, IASc, University departments and other R&D centers. 

Around 16 text books were published by the department faculty members in which two text books entitled “Hand Book of Biotechnology and Chemical Engineering†(ISBN: 978-81-224-3144-5) and “Applications of Plant Tissue Culture†(ISBN: 978-81-224-3144-6) have been published by “New Age International, New Delhi on 25th July 2010 and etc. 

In addition, a total of 155 research papers were published in National Journals and 87 research papers were published in International Journals and nearly 240 abstracts papers were published at different National and International conferences / symposia by the students and staff members. A total of five granted patents and 15 patents were filed very recently subsequently another three patents are under pipeline at national and international level especially in US and UK. Eleven DNA sequences of plant pathogenic strains were deposited at NCBI, Maryland, USA.

Ponmurugan Ponnusamy, Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India

The Department of Biotechnology, K.S.Rangasamy College of Technology is 4th ranked among the 280 private colleges offering Biotechnology programme as per "Biospectrum" survey.

The department has equiped with 11 funded projects from different funding agencies such as ICMR, DBT, DST, UGC DRDO etc.,

The department has totally 15 laboratories such as Biochemistry, Bioorganic Chemistry, Bioprocess, Plant Tissue Culture, Animal cell line, Molecular Biology/Genetic Engineering, Bioinformatics, Immunology, Cell Biology, Microbiology, Downstream processing, Cyanobacteria lab and Instrumentation. 

Around 16 students and 5 faculty memebers have received ‘Summer Research Fellowship’ from Indian Academy of Sciences (IASc), Bangalore. So far, 

21 ‘Student Project Scheme’ were received from Tamil Nadu State council of Science and Technology (TNSCST), Chennai for which a total outlay of Rs.2,80,000/- was sanctioned. A total of seven staff members were received ‘Young Scientist Award’ and ‘Summer Faculty Research Fellowship from TNSCST, Chennai and Indian Institute of Technology, New Delhi, respectively for which the recipients were carried out a project work for a period of two months at reputed institutions such as IITs, IISc, IASc, University departments and other R&D centers. 

Around 16 text books were published by the department faculty members in which two text books entitled “Hand Book of Biotechnology and Chemical Engineering†(ISBN: 978-81-224-3144-5) and “Applications of Plant Tissue Culture†(ISBN: 978-81-224-3144-6) have been published by “New Age International, New Delhi on 25th July 2010 and etc. 

In addition, a total of 155 research papers were published in National Journals and 87 research papers were published in International Journals and nearly 240 abstracts papers were published at different National and International conferences / symposia by the students and staff members. A total of five granted patents and 15 patents were filed very recently subsequently another three patents are under pipeline at national and international level especially in US and UK. Eleven DNA sequences of plant pathogenic strains were deposited at NCBI, Maryland, USA.

Sanjeeva Nayaka, Lichenology Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001
CSIR- National Botanical Research Institute, Lucknow

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Sundararaj, J. P., S. Kuppuraj, A. Ganesan, P. Ponnusamy, and S. Nayaka. “IN VITRO ASSESSSMENT OF ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS FROM LICHEN RAMALINA NERVULOSA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 8, June 2015, pp. 200-4, https://innovareacademics.in/journals/index.php/ijpps/article/view/6166.
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