IN VITRO EVALUATION OF GLYCOLIPOPROTEIN POWDER FROM EARTHWORM EUDRILUS EUGENIAE
Objective: The researchers are searching for innate bioactive compounds, competent of curing various diseases. In the present study, the cytotoxicity and the angiogenic nature of the glycolipoprotein powder from the earthworm were considered for its pharmaceutical and biological uses.
Methods: The in vitro MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay of the glycolipoprotein powder (GLP) of the earthworm in the Murine embryonic fibroblast cell line (NIH 3T3) was performed. Further the haemolytic assay of the GLP was also tested. The angiogenic potential was also evaluated by the (in vitro) chick chorioallantoic membrane (CAM) model in 9 days old fertilized chick eggs.
Results: The MTT assay was performed wherein the cell response to extract was by dose-dependent manner and great cytotoxic effect was not shown for the tested concentrations. In the MTT assay increased cell viability (84%) was found at concentrations of 100 Âµg/ml. The hemolytic activity of the extract was also determined, wherein it showed insignificant lytic activity compared to the control. On performing the CAM assay, 500 Î¼l extract (25 Î¼g/ml) was found to increase the number of capillaries on the treated CAM surfaces after 72 hours of incubation.
Conclusion: These findings suggested that the earthworm powder possesses the significant angiogenic potential, which may be beneficial in the treatment of wound healing.
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