• Cuckoo Mahapatra Cell and Developmental Biology Laboratory, P. G. Department of Zoology, Utkal University, Bhubaneswar 751004, Odisha, India
  • Anamika Patro Cell and Developmental Biology Laboratory, P. G. Department of Zoology, Utkal University, Bhubaneswar 751004, Odisha, India
  • Pravati Kumari Mahapatra Cell and Developmental Biology Laboratory, P. G. Department of Zoology, Utkal University, Bhubaneswar 751004, Odisha, India


Objective: Aim of the study was to investigate the effects of thyroxine on the pre metamorphic tadpoles of the common Asian toad, Duttaphrynus melanostictus (Anura: Bufonidae), an ideal model for understanding the role of thyroxine during vertebrate development.

Methods: Tadpoles of two developmental stages, i.e., Gosner stege 30 and 34 was exposed to 50, 100 and 200 ng/ml of thyroxine by adding the hormone to the tadpole rearing water. Toxic and teratogenic effects of thyroxine on the tadpoles were recorded. Besides, effects of thyroxine on specific activity of acid phosphatase, a lysosomal marker enzyme in the tails of the tadpoles were investigated.

Results: A Dose and stage specific toxic effect of the hormone was observed in the treated tadpoles. There was precocious metamorphosis with incomplete differentiation of limbs, deformities of body and tail in the treated tadpoles. Biochemical investigation of tails showed a dose specific elevation in the specific activity of acid phosphatase up to 2.5 fold in 50 ng/ml and 3.15 fold in 100 ng/ml treated tadpoles as compared to corresponding control tadpoles.

Conclusion: Thus, the hormone thyroxine exhibited toxic as well as teratogenic effects on the developing tadpoles and also led to dose dependent elevation in specific activity of acid phosphatase in vivo in their tails. Keywords: Acid phosphatase, Duttaphrynus melanostictus, Tail regression, Thyroxine



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Author Biography

Cuckoo Mahapatra, Cell and Developmental Biology Laboratory, P. G. Department of Zoology, Utkal University, Bhubaneswar 751004, Odisha, India

Department of Zoology



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How to Cite
Mahapatra, C., A. Patro, and P. K. Mahapatra. “DOSE AND STAGE SPECIFIC EFFECTS OF THYROXINE ON THE TADPOLES OF DUTTAPHRYNUS MELANOSTICTUS (ANURA: BUFONIDAE)”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 8, June 2015, pp. 171-5,
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