CYTOPROTECTIVE EFFECT OF OCIMUM EXTRACT ON INJURED RENAL EPITHELIAL CELLS
Objectives: Renal cell injury is one of the important factors affecting urolithiasis. Crystals which aggregate to constitute the kidney stones are formed in the supersaturation condition in kidney. Reactive oxygen species generated due to the injury of renal epithelial cell by crystal formation and the injured cells itself participate in kidney stone formation. In the present study antioxidant, anti-inflammatory and cytoprotective effect of aqueous extract of Ocimum leaves were studied.
Methods: Qualitative analysis of the plant extract was done using the basic phytochemical screening. DPPH (1, 1-diphenyl-2picrylhydrazyl) free radical scavenging activity test was done to estimate antioxidant activity of plant extract and protein denaturation method used to assess anti-inflammatory property of plant extract. The MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay was also done for the evaluation of cytoprotective effect of the plant extract on Vero cell line.
Results: The results indicated that different concentrations of aqueous extract had strong antioxidant (94.5%Â±1.8) and anti-inflammatory (80.4%Â±1.3) activity. The aqueous extract increased the cell viability of Vero cell lines in a dose dependent manner. 40Âµg of the Ocimum extract exhibited 88.35%Â±1.32 cell viability of Vero cells which were injured with calcium oxalate crystals.
Conclusion: Aqueous extract of Ocimum has cytoprotective effect on injured Vero cell lines. Anti-inflammatory and antioxidant property of Ocimum extract might be responsible for this cytoprotective nature of Ocimum extract.
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