• Priyadarshini . Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India
  • Kanika Jain Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India


Objectives: Renal cell injury is one of the important factors affecting urolithiasis. Crystals which aggregate to constitute the kidney stones are formed in the supersaturation condition in kidney. Reactive oxygen species generated due to the injury of renal epithelial cell by crystal formation and the injured cells itself participate in kidney stone formation. In the present study antioxidant, anti-inflammatory and cytoprotective effect of aqueous extract of Ocimum leaves were studied.

Methods: Qualitative analysis of the plant extract was done using the basic phytochemical screening. DPPH (1, 1-diphenyl-2picrylhydrazyl) free radical scavenging activity test was done to estimate antioxidant activity of plant extract and protein denaturation method used to assess anti-inflammatory property of plant extract. The MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay was also done for the evaluation of cytoprotective effect of the plant extract on Vero cell line.

Results: The results indicated that different concentrations of aqueous extract had strong antioxidant (94.5%±1.8) and anti-inflammatory (80.4%±1.3) activity. The aqueous extract increased the cell viability of Vero cell lines in a dose dependent manner. 40µg of the Ocimum extract exhibited 88.35%±1.32 cell viability of Vero cells which were injured with calcium oxalate crystals.

Conclusion: Aqueous extract of Ocimum has cytoprotective effect on injured Vero cell lines. Anti-inflammatory and antioxidant property of Ocimum extract might be responsible for this cytoprotective nature of Ocimum extract.

Keywords: Urolithiasis, Ocimum, Antioxidant, Anti-inflammatory, Crystal, Vero cell line


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1. Romero V, Akpinar H, Assimos DG. Kidney stones: a global picture of prevalence, incidence, and associated risk factors. Rev Urol 2010;12:2-3.
2. Evan AP. Physiopathology and etiology of stone formation in the kidney and the urinary tract. Pediatr Nephrol 2010;25 Suppl 5:831-41.
3. Finkielstein VA, Goldfarb DS. Strategies for preventing calcium oxalate stones. Can Med Assoc J 2006;174:1407-9.
4. Emin Ozbek. Induction of oxidative stress in kidney. Int J Nephrol 2012. [Article in Press]
5. Alok S, Jain SK, Verma A Kumar, Sabharwal M. Pathophysiology of kidney, gallbladder and urinary stones treatment with herbal and allopathic medicine: a review. Asian Pac J Trop Dis 2013;3:496-04.
6. Shimizu T, Torres MP, Chakraborty S, Souchek JJ, Rachagani S, Kaur S, et al. Holy basil leaf extract decreases tumorigenicity and metastasis of aggressive human pancreatic cancer cells in vitro and in vivo: potential role in therapy. Cancer Lett 2013;336:270-80.
7. Pattanayak P, Behera P, Das D, Panda SK. Ocimum sanctum Linn. A reservoir plant for therapeutic applications: an overview. Pharmacogn Rev 2010;4:95-105.
8. Showkat AW, Shah KW, Mir AA. Preliminary phytochemical investigation and thin layer chromatography of Rheum emodi. Int Res J Pharm 2012;3:4.
9. Chang C, Yang M, Wen H, Chern JJ. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.
10. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebenson Wiss Technol 1995;28:25-30.
11. Padmanabhan P, Jangle SN. Evaluation of in-vitro anti-inflammatory activity of herbal preparation, a combination of four herbal plants. Int J Appl Basic Med Res 2012;2:109-16.
12. Alhakmani F, Kumar S, Khan SA. Estimation of total phenolic content, in in-vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera. Asian Pac J Trop Biomed 2013;3:623-27.
13. Karamustafa F, Çelebi N, Değim Z, Şyilmaz Ş. Evaluation of the viability of L-929 cells in the presence of alendronate and absorption enhancers. FABAD J Pharm Sci 2006;31:1-5.
14. Bangaruswamy D, Jambunathan S, Padma PR, Sundaradivelu S. In vitro antioxidant activity of Prosopis cineraria leaves against H2O2 induced oxidative stress in goat liver slices. Int J Pharm Pharm Sci 2015;7:35-7.
15. Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pac J Trop Biomed 2012;2(1, Suppl): S178-80.
16. Jaggi RK, Madaan R, Singh B. Anticonvulsant potential of holy basil, Ocimum sanctum Linn. and its cultures. Indian J Exp Biol 2003;41:1329–33.
17. Gupta SK, Prakash J, Srivastava S. Validation of traditional claim of Tulsi, Ocimum sanctum Linn. as a medicinal plant. Indian J Exp Biol 2002;40:765-73.
18. Aggarwal A, Tandon S, Singla SK, Tandon C. Reduction of oxalate-induced renal tubular epithelial (NRK-52E) cell injury and inhibition of calcium oxalate crystallisation in vitro by aqueous extract of Achyranthes aspera. Int J Green Pharm 2010;4:159-64.
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How to Cite
., P., and K. Jain. “CYTOPROTECTIVE EFFECT OF OCIMUM EXTRACT ON INJURED RENAL EPITHELIAL CELLS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 11, Aug. 2015, pp. 15-18,
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